Literature DB >> 8395540

A new method of RNA preparation for detection of hepatitis A virus in environmental samples by the polymerase chain reaction.

F Yang1, X Xu.   

Abstract

A new method based on RNA preparation from shellfish by polyethylene glycol (PEG) precipitation and trichloroacetic acid (TCA) extraction was developed for the detection of hepatitis A virus (HAV) by the polymerase chain reaction (PCR). The extraction provides high yield and the extracted RNA is undegraded. This method proved to be particularly useful for detection of RNA viruses from environmental samples by PCR.

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Year:  1993        PMID： 8395540     DOI： 10.1016/0166-0934(93)90091-5

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  6 in total

1.  Development of a concentration method for detection of tobacco mosaic virus in irrigation water.

Authors:  Wei Chen; Wenting Liu; Honghong Jiao; Huawei Zhang; Julong Cheng; Yunfeng Wu
Journal:  Virol Sin       Date:  2014-06-18       Impact factor: 4.327

2.  Detection of hepatitis A virus RNA in oyster meat.

Authors:  T L Cromeans; O V Nainan; H S Margolis
Journal:  Appl Environ Microbiol       Date:  1997-06       Impact factor: 4.792

3.  Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR.

Authors:  K J Schwab; R De Leon; M D Sobsey
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

4.  Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR.

Authors:  K J Schwab; R De Leon; M D Sobsey
Journal:  Appl Environ Microbiol       Date:  1996-06       Impact factor: 4.792

5.  Detection of hepatitis A virus in environmental samples by antigen-capture PCR.

Authors:  M Y Deng; S P Day; D O Cliver
Journal:  Appl Environ Microbiol       Date:  1994-06       Impact factor: 4.792

6.  In situ detection of hepatitis A virus in cell cultures and shellfish tissues.

Authors:  J L Romalde; M K Estes; G Szücs; R L Atmar; C M Woodley; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1994-06       Impact factor: 4.792
  6 in total

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