OBJECTIVE: This study measured platelet activating factor (PAF) production by rabbit embryos in vitro and ascertained if there is increased PAF production associated with advancing embryonic development. STUDY DESIGN: Two-cell rabbit embryos were recovered from superovulated New Zealand White does and cultured in vitro for 96 hr. Every 24 hr embryos were scored for developmental stage and PAF activity from the corresponding culture medium was measured by platelet aggregation and organic phosphate analyses. RESULTS: PAF was detected in culture medium at all stages from two cells to blastocysts and rose significantly (P < 0.001) at each 24-hr interval, reaching maximal levels at the expanded blastocyst stage. CONCLUSION: Maximal PAF production by expanded blastocysts may be an embryonic paracrine signal that facilitates implantation.
OBJECTIVE: This study measured platelet activating factor (PAF) production by rabbit embryos in vitro and ascertained if there is increased PAF production associated with advancing embryonic development. STUDY DESIGN: Two-cell rabbit embryos were recovered from superovulated New Zealand White does and cultured in vitro for 96 hr. Every 24 hr embryos were scored for developmental stage and PAF activity from the corresponding culture medium was measured by platelet aggregation and organicphosphate analyses. RESULTS: PAF was detected in culture medium at all stages from two cells to blastocysts and rose significantly (P < 0.001) at each 24-hr interval, reaching maximal levels at the expanded blastocyst stage. CONCLUSION: Maximal PAF production by expanded blastocysts may be an embryonic paracrine signal that facilitates implantation.
Authors: Elissa T Purnell; Carol M Warner; Hilton I Kort; Dorothy Mitchell-Leef; Carlene W Elsner; Daniel B Shapiro; Joe B Massey; William E Roudebush Journal: J Assist Reprod Genet Date: 2006-06-24 Impact factor: 3.412
Authors: William E Roudebush; Joe B Massey; Hilton I Kort; Carlene W Elsner; Andrew A Toledo; Dorothy Mitchell-Leef; Daniel B Shapiro Journal: J Assist Reprod Genet Date: 2004-08 Impact factor: 3.412