Literature DB >> 3341753

Occurrence of platelet-activating factor in rabbit spermatozoa.

R Kumar1, M J Harper, D J Hanahan.   

Abstract

Spermatozoa obtained from rabbit ejaculate were analyzed for the presence of platelet-activating factor [PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC)] by using standard HPLC and TLC procedures. Fractions corresponding to synthetic PAF (AGEPC) revealed PAF-like activity amounting to 0.35 +/- 0.06 pmol/10(8) cells (mean +/- SE) as determined by bioassays based on the release of [3H]serotonin from washed rabbit platelets. This activity was lost upon base-catalyzed methanolysis, but was restored to the original level after reacetylation. Analysis of the phosphatidylcholine (PC) fraction by GC-MS subsequent to base-catalyzed methanolysis showed that 1-O-alkyl-2-acylphosphocholine comprises about 12% of the PC fraction with alkyl chain lengths of 16:0 (88%) and 18:0 (12%).

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Year:  1988        PMID： 3341753     DOI： 10.1016/0003-9861(88)90474-2

Source DB:  PubMed          Journal:  Arch Biochem Biophys        ISSN： 0003-9861            Impact factor:   4.013


  7 in total

1.  Platelet activating factor treatment of spermatozoa enhances rabbit oocyte fertilization following subzonal sperm insertion.

Authors:  B S Minhas; H N Kim; Y P Zhu; B A Ripps; T H Burwinkel
Journal:  J Assist Reprod Genet       Date:  1994-07       Impact factor: 3.412

2.  Embryonic platelet activating factor production in the rabbit increases during the preimplantation phase.

Authors:  B S Minhas; Y P Zhu; H N Kim; T H Burwinkel; B A Ripps; J E Buster
Journal:  J Assist Reprod Genet       Date:  1993-07       Impact factor: 3.412

3.  Enhanced embryo development of rabbit oocytes fertilized in vitro with platelet activating factor (PAF)-treated spermatozoa.

Authors:  W E Roudebush; A I Fukuda; B S Minhas
Journal:  J Assist Reprod Genet       Date:  1993-01       Impact factor: 3.412

4.  Effects of platelet activating factor on mouse sperm function.

Authors:  K Sengoku; M Ishikawa; K Tamate; T Shimizu
Journal:  J Assist Reprod Genet       Date:  1992-10       Impact factor: 3.412

5.  The hormonal regulation of platelet-activating factor acetylhydrolase activity in plasma.

Authors:  S Miyaura; N Maki; W Byrd; J M Johnston
Journal:  Lipids       Date:  1991-12       Impact factor: 1.880

6.  Stimulation of platelet-activating factor synthesis by progesterone and A23187 in human spermatozoa.

Authors:  E Baldi; C Falsetti; C Krausz; G Gervasi; V Carloni; R Casano; G Forti
Journal:  Biochem J       Date:  1993-05-15       Impact factor: 3.857

Review 7.  Sperm preparation for ART.

Authors:  Ralf R Henkel; Wolf-Bernhard Schill
Journal:  Reprod Biol Endocrinol       Date:  2003-11-14       Impact factor: 5.211
  7 in total

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