W E Roudebush1, S Mathur, W J Butler. 1. Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston 29425-2233, USA.
Abstract
OBJECTIVE: Our purpose was to investigate the effect of anti-PAF antibodies on CFW mouse embryo development in vitro. DESIGN: We studied the in vitro development of CFW mouse one-cell-stage embryos cultured in MEM supplemented with anti-PAF, anti-IgG, or MEM alone to the hatched blastocyst stage. RESULTS: Mouse embryos cultured with anti-PAF (1:5 dilution; 61%) significantly decreased embryo development compared to controls (MEM alone; 93%), whereas embryos cultured in anti-mouse IgG-supplemented MEM (1:10 dilution; 93%) had no effect. CONCLUSIONS: The results provide additional evidence that PAF is produced and secreted by cleavage-stage embryos and is required during the preimplantation period.
OBJECTIVE: Our purpose was to investigate the effect of anti-PAF antibodies on CFW mouse embryo development in vitro. DESIGN: We studied the in vitro development of CFW mouse one-cell-stage embryos cultured in MEM supplemented with anti-PAF, anti-IgG, or MEM alone to the hatched blastocyst stage. RESULTS:Mouse embryos cultured with anti-PAF (1:5 dilution; 61%) significantly decreased embryo development compared to controls (MEM alone; 93%), whereas embryos cultured in anti-mouseIgG-supplemented MEM (1:10 dilution; 93%) had no effect. CONCLUSIONS: The results provide additional evidence that PAF is produced and secreted by cleavage-stage embryos and is required during the preimplantation period.
Authors: William E Roudebush; Joe B Massey; Hilton I Kort; Carlene W Elsner; Andrew A Toledo; Dorothy Mitchell-Leef; Daniel B Shapiro Journal: J Assist Reprod Genet Date: 2004-08 Impact factor: 3.412