Literature DB >> 7894159

Characterization of pig-mink cell hybrids: assignment of the TK1 and UMPH2 genes to pig chromosome 12.

N S Zhdanova1, N M Astakhova, S B Kuznetsov, L Schuler, O L Serov.   

Abstract

By fusion of thymidine kinase-deficient mink cells with pig leukocytes, a new type of cell hybrid was produced. It was demonstrated that pig chromosomes segregate in pig-mink hybrids and that hybrid cells contain no cytologically visible rearrangements between the chromosomes of parental species, or chromosome fragmentation. With a set of subclones of two primary hybrid clones, the genes for thymidine kinase-1 (TK1) and uridine 5'-monophosphate hydrolase-2 (UMPH2) were assigned to pig Chromosome (Chr) 12. A cell line with a single pig Chr 8 on the background of mink chromosomes was established. This clone could serve as a source of DNA for building a chromosome-specific library of pig Chr 8. The data obtained suggest that pig-mink cell hybrids can be used for mapping of pig chromosomes.

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Year:  1994        PMID： 7894159     DOI： 10.1007/bf00292013

Source DB:  PubMed          Journal:  Mamm Genome        ISSN： 0938-8990            Impact factor:   2.957


  13 in total

1.  The high-resolution GTG-banding pattern of pig chromosomes.

Authors:  M Yerle; O Galman; G Echard
Journal:  Cytogenet Cell Genet       Date:  1991

2.  Chromosomal and regional localization of the genes for UMPH2, APRT, PEPD, PEPS, PSP, and PGP in mink: comparison with man and mouse.

Authors:  S D Pack; N S Zhdanova; M A Sukoyan; O L Serov
Journal:  Cytogenet Cell Genet       Date:  1989

3.  Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol.

Authors:  R L Davidson; P S Gerald
Journal:  Somatic Cell Genet       Date:  1976-03

4.  Regional assignment of the genes for TK1, GALK, ALDC, and ESD on chromosome 8 in the American mink by chromosome-mediated gene transfer.

Authors:  A A Gradov; S D Pack; M A Sukoyan; N B Rubtsov; M N Bochkarev; O L Serov
Journal:  Mol Gen Genet       Date:  1985

5.  Assignment of superoxide dismutase (SOD-1) gene to chromosome No. 9 of domestic pig.

Authors:  M M Leong; C C Lin; R F Ruth
Journal:  Can J Genet Cytol       Date:  1983-06

6.  Regional assignments of NP and MPI on chromosome 7 in pig, Sus scrofa.

Authors:  H Ryttman; P Thebo; I Gustavsson
Journal:  Anim Genet       Date:  1988       Impact factor: 3.169

7.  The porcine PGD gene is preferentially lost from chromosome 6 in pig x rodent somatic cell hybrids.

Authors:  P D Thomsen; A A Bosma; U Kaufmann; I Harbitz
Journal:  Hereditas       Date:  1991       Impact factor: 3.271

8.  Further data on chromosomal assignments of pig enzyme loci LDHA, LDHB, MPI, PEPB and PGM1, using somatic cell hybrids.

Authors:  H Ryttman; P Thebo; I Gustavsson; B Gahne; R K Juneja
Journal:  Anim Genet       Date:  1986       Impact factor: 3.169

9.  Standard karyotype of the domestic pig. Committee for the Standardized Karyotype of the Domestic Pig.

Authors:  I Gustavsson
Journal:  Hereditas       Date:  1988       Impact factor: 3.271

10.  Chromosome localization of the loci GOT1, PP, NP, SOD1, PEPA and PEPC in the American mink (Mustela vison).

Authors:  N B Rubtsov; A A Gradov; O L Serov
Journal:  Theor Appl Genet       Date:  1982-12       Impact factor: 5.699
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  5 in total

1.  Production of pig-mink cell hybrids with single pig chromosomes 2, 5, 12, or t(1,13).

Authors:  N S Zhdanova; P D Thomsen; N M Astakhova; S B Kuznetsov; C B Jörgensen; E V Plyusnina; O L Serov
Journal:  Mamm Genome       Date:  1996-08       Impact factor: 2.957

2.  Construction of a cytogenetically characterized porcine somatic cell hybrid panel and its use as a mapping tool.

Authors:  C Zijlstra; A A Bosma; N A de Haan; C Mellink
Journal:  Mamm Genome       Date:  1996-04       Impact factor: 2.957

3.  The cytogenetic map of the domestic pig.

Authors:  M Yerle; Y Lahbib-Mansais; P Pinton; A Robic; A Goureau; D Milan; J Gellin
Journal:  Mamm Genome       Date:  1997-08       Impact factor: 2.957

4.  Reverse painting for identification of pig chromosomes in hybrid cell lines: assignment of the HOXB and the TK1 gene to pig chromosome 12p.

Authors:  P D Thomsen; N S Zhdanova
Journal:  Mamm Genome       Date:  1995-09       Impact factor: 2.957

5.  Chromosomal and regional localization of the loci for IGKC, IGGC, ALDB, HOXB, GPT, and PRNP in the American mink (Mustela vison): comparisons with human and mouse.

Authors:  T M Khlebodarova; S N Malchenko; N M Matveeva; S D Pack; O V Sokolova; B Y Alabiev; E S Belousov; V V Peremislov; A M Nayakshin; K Brusgaard
Journal:  Mamm Genome       Date:  1995-10       Impact factor: 2.957
  5 in total

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