Literature DB >> 1028164

Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol.

R L Davidson, P S Gerald.   

Abstract

Modifications in the techniques for the induction of mammalian somatic cell hybridization by polyethylene glycol (PEG) have led to procedures that are rapid, simple, and effective. The basic improvements, for both monolayer and suspension fusions, are a short exposure to PEG and a rapid dilution of PEG following treatment. There is a marked effect of PEG concentration on cell hybridization, and there seem to be inherent differences between cells in terms of the extent of cell fusion induced by PEG.

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Year:  1976        PMID： 1028164     DOI： 10.1007/BF01542629

Source DB:  PubMed          Journal:  Somatic Cell Genet        ISSN： 0098-0366


  138 in total

1.  Adenovirus late gene expression does not require a Rev-like nuclear RNA export pathway.

Authors:  C Rabino; A Aspegren; K Corbin-Lickfett; E Bridge
Journal:  J Virol       Date:  2000-07       Impact factor: 5.103

2.  An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression.

Authors:  Amy M Chastain-Moore; Terry Roberts; Deborah A Trott; Robert F Newbold; David A Ornelles
Journal:  J Virol       Date:  2003-07       Impact factor: 5.103

3.  A single mutation affects both N-acetylglucosaminyltransferase and glucuronosyltransferase activities in a Chinese hamster ovary cell mutant defective in heparan sulfate biosynthesis.

Authors:  K Lidholt; J L Weinke; C S Kiser; F N Lugemwa; K J Bame; S Cheifetz; J Massagué; U Lindahl; J D Esko
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-15       Impact factor: 11.205

4.  Linkage analysis of Norrie disease with X-chromosomal ornithine aminotransferase.

Authors:  J B Bateman
Journal:  Trans Am Ophthalmol Soc       Date:  1992

5.  Formation of multilayer rosettes in phytohemagglutinin-stimulated lymphocytes: correlation with chromatin condensation conformation in premature chromosome condensation.

Authors:  H Thomou; C Sambani; P Kitsiou; G Politis
Journal:  Cytotechnology       Date:  1988-07       Impact factor: 2.058

6.  Transfer of glucagon receptor from liver membranes to a foreign adenylate cyclase by a membrane fusion procedure.

Authors:  M Schramm
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

7.  Deleted chromosome 20 from a patient with Alagille syndrome isolated in a cell hybrid through leucine transport selection: study of three candidate genes.

Authors:  J F Deleuze; S Dhorne; J Hazan; E Borghi; N Raynaud; N Pollet; M Meunier-Rotival; J Deschatrette; D Alagille; M Hadchouel
Journal:  Mamm Genome       Date:  1994-11       Impact factor: 2.957

8.  Assignment of the gene for methylthioadenosine phosphorylase to human chromosome 9 by mouse-human somatic cell hybridization.

Authors:  C J Carrera; R L Eddy; T B Shows; D A Carson
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

9.  Chromosome localization and gene synteny of the major histocompatibility complex in the owl monkey, Aotus.

Authors:  N S Ma; T Simeone; J McLean; P Parham
Journal:  Immunogenetics       Date:  1982-01       Impact factor: 2.846

10.  Selection of reconstituted cells from karyoplasts fused to chloramphenicol-resistant cytoplasts.

Authors:  J W Shay
Journal:  Proc Natl Acad Sci U S A       Date:  1977-06       Impact factor: 11.205
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