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Literature DB >> 8661693

Construction of a cytogenetically characterized porcine somatic cell hybrid panel and its use as a mapping tool.

C Zijlstra¹, A A Bosma, N A de Haan, C Mellink.

Abstract

A new panel of cytogenetically characterized pig-rodent somatic cell hybrids was constructed and tested for twelve microsatellite markers with PCR. Cytogenetic characterization of hybrids was accomplished by fluorescence painting and GTG-banding of metaphase chromosomes. The panel consists of 15 independent pig-hamster and 6 independent pig-mouse cell lines. In the panel, all pig autosomes and the X Chromosome (Chr) are represented, and it is informative for all chromosome pairs except 2-14, 2-15, 3-9, 14-15, 14-16, and 16-17. The microsatellites tested were S0022, S0023, S0084, S0098, S0112, S0113, S0114, S0115, S0117, S0118, S0119, and S0120. The PCR results obtained in the 21 hybrids were compared with the cytogenetic data and analyzed for concordancy and correlation. Eight microsatellites could be assigned to specific pig chromosomes, confirming seven assignments based on linkage analysis.

Entities: Species

Mesh：

Year: 1996 PMID： 8661693 DOI： 10.1007/s003359900083

Source DB: PubMed Journal: Mamm Genome ISSN： 0938-8990 Impact factor: 2.957

19 in total

1. Statistical decision rules concerning synteny or independence between markers.

Authors: C Chevalet; F Corpet
Journal: Cytogenet Cell Genet Date: 1986

2. Development and mapping of ten porcine microsatellite markers.

Authors: M A Groenen; D Ruyter; E J Verstege; M de Vries; J J van der Poel
Journal: Anim Genet Date: 1995-04 Impact factor: 3.169

3. Assignment of 20 microsatellite markers to the porcine linkage map.

Authors: H Ellegren; M Johansson; B P Chowdhary; S Marklund; D Ruyter; L Marklund; P Bräuner-Nielsen; I Edfors-Lilja; I Gustavsson; R K Juneja
Journal: Genomics Date: 1993-05 Impact factor: 5.736

4. Gene mapping in the rat by mouse-rat somatic cell hybridization: synteny of the albumin and alpha-fetoprotein genes and assignment to chromosome 14.

Authors: J Szpirer; G Levan; M Thörn; C Szpirer
Journal: Cytogenet Cell Genet Date: 1984

5. Chromosomal assignment of 11 loci in the rat by mouse-rat somatic hybrids and linkage.

Authors: A Y Deng; L Gu; J P Rapp; C Szpirer; J Szpirer
Journal: Mamm Genome Date: 1994-11 Impact factor: 2.957

6. Characterization of porcine polymorphic microsatellite loci.

Authors: W Coppieters; A Van de Weghe; L Peelman; A Depicker; A Van Zeveren; Y Bouquet
Journal: Anim Genet Date: 1993-06 Impact factor: 3.169

7. Characterization of a new hybrid mink-mouse clone panel: chromosomal and regional assignments of the GLO, ACY, NP, CKBB, ADH2, and ME1 loci in mink (Mustela vison).

Authors: S D Pack; V M Bedanov; O V Sokolova; N S Zhdanova; N M Matveeva; O L Serov
Journal: Mamm Genome Date: 1992 Impact factor: 2.957

Construction of a cytogenetically characterized porcine somatic cell hybrid panel and its use as a mapping tool.

1. Statistical decision rules concerning synteny or independence between markers.

2. Development and mapping of ten porcine microsatellite markers.

3. Assignment of 20 microsatellite markers to the porcine linkage map.

4. Gene mapping in the rat by mouse-rat somatic cell hybridization: synteny of the albumin and alpha-fetoprotein genes and assignment to chromosome 14.

5. Chromosomal assignment of 11 loci in the rat by mouse-rat somatic hybrids and linkage.

6. Characterization of porcine polymorphic microsatellite loci.

7. Characterization of a new hybrid mink-mouse clone panel: chromosomal and regional assignments of the GLO, ACY, NP, CKBB, ADH2, and ME1 loci in mink (Mustela vison).

8. Localization of the human HTF4 transcription factors 4 gene (TCF12) to chromosome 15q21.

9. Comparative study of pig-rodent somatic cell hybrids.

10. Standard karyotype of the domestic pig. Committee for the Standardized Karyotype of the Domestic Pig.

1. The cytogenetic map of the domestic pig.

2. Mapping of microsatellite markers developed from a flow-sorted swine chromosome 6 library.