Literature DB >> 7756995

Disulfide crosslinks to probe the structure and flexibility of a designed four-helix bundle protein.

L Regan1, A Rockwell, Z Wasserman, W DeGrado.   

Abstract

The introduction of disulfide crosslinks is a generally useful method by which to identify regions of a protein that are close together in space. Here we describe the use of disulfide crosslinks to investigate the structure and flexibility of a family of designed 4-helix bundle proteins. The results of these analyses lend support to our working model of the proteins' structure and suggest that the proteins have limited main-chain flexibility.

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Year:  1994        PMID： 7756995      PMCID： PMC2142780          DOI： 10.1002/pro.5560031225

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  25 in total

1.  Substantial increase of protein stability by multiple disulphide bonds.

Authors:  M Matsumura; G Signor; B W Matthews
Journal:  Nature       Date:  1989-11-16       Impact factor: 49.962

2.  Residual structure in large fragments of staphylococcal nuclease: effects of amino acid substitutions.

Authors:  D Shortle; A K Meeker
Journal:  Biochemistry       Date:  1989-02-07       Impact factor: 3.162

Review 3.  The molten globule state as a clue for understanding the folding and cooperativity of globular-protein structure.

Authors:  K Kuwajima
Journal:  Proteins       Date:  1989

4.  Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation.

Authors:  D Shortle; A K Meeker
Journal:  Proteins       Date:  1986-09

5.  Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants.

Authors:  M M Santoro; D W Bolen
Journal:  Biochemistry       Date:  1988-10-18       Impact factor: 3.162

6.  Characterization of a helical protein designed from first principles.

Authors:  L Regan; W F DeGrado
Journal:  Science       Date:  1988-08-19       Impact factor: 47.728

7.  Urea dependence of thiol-disulfide equilibria in thioredoxin: confirmation of the linkage relationship and a sensitive assay for structure.

Authors:  T Y Lin; P S Kim
Journal:  Biochemistry       Date:  1989-06-13       Impact factor: 3.162

8.  Structure of a thermostable disulfide-bridge mutant of phage T4 lysozyme shows that an engineered cross-link in a flexible region does not increase the rigidity of the folded protein.

Authors:  P E Pjura; M Matsumura; J A Wozniak; B W Matthews
Journal:  Biochemistry       Date:  1990-03-13       Impact factor: 3.162

9.  An engineered disulfide bond in dihydrofolate reductase.

Authors:  J E Villafranca; E E Howell; S J Oatley; N H Xuong; J Kraut
Journal:  Biochemistry       Date:  1987-04-21       Impact factor: 3.162

10.  Conformational stability and activity of ribonuclease T1 with zero, one, and two intact disulfide bonds.

Authors:  C N Pace; G R Grimsley; J A Thomson; B J Barnett
Journal:  J Biol Chem       Date:  1988-08-25       Impact factor: 5.157
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  7 in total

1.  A single disulfide bond restores thermodynamic and proteolytic stability to an extensively mutated protein.

Authors:  K R Roesler; A G Rao
Journal:  Protein Sci       Date:  2000-09       Impact factor: 6.725

2.  Controlled protein dimerization through hybrid coordination motifs.

Authors:  Robert J Radford; Phuong C Nguyen; Treffly B Ditri; Joshua S Figueroa; F Akif Tezcan
Journal:  Inorg Chem       Date:  2010-05-03       Impact factor: 5.165

3.  Introduction of a [4Fe-4S (S-cys)4]+1,+2 iron-sulfur center into a four-alpha helix protein using design parameters from the domain of the Fx cluster in the Photosystem I reaction center.

Authors:  M P Scott; J Biggins
Journal:  Protein Sci       Date:  1997-02       Impact factor: 6.725

4.  Surface point mutations that significantly alter the structure and stability of a protein's denatured state.

Authors:  C K Smith; Z Bu; K S Anderson; J M Sturtevant; D M Engelman; L Regan
Journal:  Protein Sci       Date:  1996-10       Impact factor: 6.725

5.  Use of thiol-disulfide equilibria to measure the energetics of assembly of transmembrane helices in phospholipid bilayers.

Authors:  Lidia Cristian; James D Lear; William F DeGrado
Journal:  Proc Natl Acad Sci U S A       Date:  2003-12-01       Impact factor: 11.205

6.  Determination of membrane protein stability via thermodynamic coupling of folding to thiol-disulfide interchange.

Authors:  Lidia Cristian; James D Lear; William F DeGrado
Journal:  Protein Sci       Date:  2003-08       Impact factor: 6.725

Review 7.  De novo protein design, a retrospective.

Authors:  Ivan V Korendovych; William F DeGrado
Journal:  Q Rev Biophys       Date:  2020-02-11       Impact factor: 5.318
  7 in total

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