Literature DB >> 7568117

Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution.

J R Tolman1, J M Flanagan, M A Kennedy, J H Prestegard.   

Abstract

The measurement of dipolar contributions to the splitting of 15N resonances of 1H-15N amide pairs in multidimensional high-field NMR spectra of field-oriented cyanometmyoglobin is reported. The splittings appear as small field-dependent perturbations of normal scalar couplings. Assignment of more than 90 resonances to specific sequential sites in the protein allows correlation of the dipolar contributions with predictions based on the known susceptibility and known structure of the protein. Implications as an additional source of information for protein structure determination in solution are discussed.

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Year:  1995        PMID： 7568117      PMCID： PMC40968          DOI： 10.1073/pnas.92.20.9279

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  10 in total

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Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

2.  Evaluation of dipolar nuclear magnetic resonance shifts in low-spin hemin systems: ferricytochrome c and metmyoglobin cyanide.

Authors:  W D Horrocks; E S Greenberg
Journal:  Biochim Biophys Acta       Date:  1973-09-21

Review 3.  Two-dimensional nuclear magnetic resonance spectra of paramagnetic systems.

Authors:  L Banci; I Bertini; C Luchinat
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

Review 4.  Practical introduction to theory and implementation of multinuclear, multidimensional nuclear magnetic resonance experiments.

Authors:  A S Edison; F Abildgaard; W M Westler; E S Mooberry; J L Markley
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

5.  Sequential resonance assignments as a basis for determination of spatial protein structures by high resolution proton nuclear magnetic resonance.

Authors:  K Wüthrich; G Wider; G Wagner; W Braun
Journal:  J Mol Biol       Date:  1982-03-05       Impact factor: 5.469

6.  Characterization of heme orientational disorder in myoglobin by proton nuclear Overhauser effects.

Authors:  J T Lecomte; R D Johnson; G N La Mar
Journal:  Biochim Biophys Acta       Date:  1985-06-10

7.  Solution structural characteristics of cyanometmyoglobin: resonance assignment of heme cavity residues by two-dimensional NMR.

Authors:  S D Emerson; G La Mar
Journal:  Biochemistry       Date:  1990-02-13       Impact factor: 3.162

8.  NMR determination of the orientation of the magnetic susceptibility tensor in cyanometmyoglobin: a new probe of steric tilt of bound ligand.

Authors:  S D Emerson; G N La Mar
Journal:  Biochemistry       Date:  1990-02-13       Impact factor: 3.162

9.  Neutron diffraction study of carbonmonoxymyoglobin.

Authors:  X D Cheng; B P Schoenborn
Journal:  J Mol Biol       Date:  1991-07-20       Impact factor: 5.469

10.  1H and 15N resonance assignments and secondary structure of the carbon monoxide complex of sperm whale myoglobin.

Authors:  Y Thériault; T C Pochapsky; C Dalvit; M L Chiu; S G Sligar; P E Wright
Journal:  J Biomol NMR       Date:  1994-07       Impact factor: 2.835
  10 in total
  262 in total

1.  Calculations of NMR dipolar coupling strengths in model peptides.

Authors:  D A Case
Journal:  J Biomol NMR       Date:  1999-10       Impact factor: 2.835

2.  Sign determination of dipolar couplings in field-oriented bicelles by variable angle sample spinning (VASS).

Authors:  F Tian; J A Losonczi; M W Fischer; J H Prestegard
Journal:  J Biomol NMR       Date:  1999-10       Impact factor: 2.835

3.  Characterization of surfactant liquid crystal phases suitable for molecular alignment and measurement of dipolar couplings.

Authors:  L G Barrientos; C Dolan; A M Gronenborn
Journal:  J Biomol NMR       Date:  2000-04       Impact factor: 2.835

4.  A new approach for applying residual dipolar couplings as restraints in structure elucidation.

Authors:  J Meiler; N Blomberg; M Nilges; C Griesinger
Journal:  J Biomol NMR       Date:  2000-03       Impact factor: 2.835

5.  Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously.

Authors:  E de Alba; M Suzuki; N Tjandra
Journal:  J Biomol NMR       Date:  2001-01       Impact factor: 2.835

6.  Solution NMR of proteins within polyacrylamide gels: diffusional properties and residual alignment by mechanical stress or embedding of oriented purple membranes.

Authors:  H J Sass; G Musco; S J Stahl; P T Wingfield; S Grzesiek
Journal:  J Biomol NMR       Date:  2000-12       Impact factor: 2.835

7.  A method for incorporating dipolar couplings into structure calculations in cases of (near) axial symmetry of alignment.

Authors:  G A Mueller; W Y Choy; N R Skrynnikov; L E Kay
Journal:  J Biomol NMR       Date:  2000-11       Impact factor: 2.835

8.  A doublet-separated sensitivity-enhanced HSQC for the determination of scalar and dipolar one-bond J-couplings.

Authors:  F Cordier; A J Dingley; S Grzesiek
Journal:  J Biomol NMR       Date:  1999-02       Impact factor: 2.835

9.  The use of dipolar couplings for determining the solution structure of rat apo-S100B(betabeta).

Authors:  A C Drohat; N Tjandra; D M Baldisseri; D J Weber
Journal:  Protein Sci       Date:  1999-04       Impact factor: 6.725

10.  A new method for the simultaneous measurement of magnitude and sign of 1DCH and 1DHH dipolar couplings in methylene groups.

Authors:  T Carlomagno; W Peti; C Griesinger
Journal:  J Biomol NMR       Date:  2000-06       Impact factor: 2.835
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