Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A doublet-separated sensitivity-enhanced HSQC for the determination of scalar and dipolar one-bond J-couplings.

Literature DB >> 10070758

A doublet-separated sensitivity-enhanced HSQC for the determination of scalar and dipolar one-bond J-couplings.

Abstract

A simple, sensitivity-enhanced HSQC experiment is described which separates the upfield and downfield components in the indirect dimension into different subspectra. The sequence is similar to the generalized TROSY scheme; however, decoupling of the X-nucleus is used during detection. A detailed analysis of relaxation effects, precision and sensitivity of the method is presented. The approach is demonstrated in a two-dimensional water flip-back 1H-15N HSQC which measures 1JHN splittings in isotropic and oriented samples of ubiquitin and the hepatitis C protease. The results are in excellent agreement with splittings obtained from a conventional 1H-coupled HSQC.

Entities: Chemical Disease

Mesh：

Substances：
Protons

Year: 1999 PMID： 10070758 DOI： 10.1023/a:1008301415843

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

15 in total

1. Integration of spin-state-selective excitation into 2D NMR correlation experiments with the heteronuclear ZQ/2Q pi rotations for 1JXH- resolved E.COSY-type measurements of heteronuclear coupling constants in proteins.

Authors: A Meissner; J O Duus; O W Sørensen
Journal: J Biomol NMR Date: 1997-07 Impact factor: 2.835

2. High-resolution heteronuclear NMR of human ubiquitin in an aqueous liquid crystalline medium.

Authors: A Bax; N Tjandra
Journal: J Biomol NMR Date: 1997-10 Impact factor: 2.835

Review 3. Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra.

Authors: M Ottiger; F Delaglio; A Bax
Journal: J Magn Reson Date: 1998-04 Impact factor: 2.229

4. Magnetic field induced ordering of bile salt/phospholipid micelles: new media for NMR structural investigations.

Authors: P Ram; J H Prestegard
Journal: Biochim Biophys Acta Date: 1988-05-24

5. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors: F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal: J Biomol NMR Date: 1995-11 Impact factor: 2.835

6. Single Transition-to-single Transition Polarization Transfer (ST2-PT) in [15N,1H]-TROSY.

Authors: K V Pervushin; G Wider; K Wüthrich
Journal: J Biomol NMR Date: 1998-08 Impact factor: 2.835

7. Characterization of magnetically oriented phospholipid micelles for measurement of dipolar couplings in macromolecules.

Authors: M Ottiger; A Bax
Journal: J Biomol NMR Date: 1998-10 Impact factor: 2.835

8. Heteronuclear correlation experiments for the determination of one-bond coupling constants.

Authors: P Andersson; K Nordstrand; M Sunnerhagen; E Liepinsh; I Turovskis; G Otting
Journal: J Biomol NMR Date: 1998-05 Impact factor: 2.835

9. Characterization of magnetically orientable bilayers in mixtures of dihexanoylphosphatidylcholine and dimyristoylphosphatidylcholine by solid-state NMR.

Authors: C R Sanders; J P Schwonek
Journal: Biochemistry Date: 1992-09-22 Impact factor: 3.162

10. Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution.

Authors: J R Tolman; J M Flanagan; M A Kennedy; J H Prestegard
Journal: Proc Natl Acad Sci U S A Date: 1995-09-26 Impact factor: 11.205

47 in total

1. A new approach for applying residual dipolar couplings as restraints in structure elucidation.

Authors: J Meiler; N Blomberg; M Nilges; C Griesinger
Journal: J Biomol NMR Date: 2000-03 Impact factor: 2.835

2. Solution NMR of proteins within polyacrylamide gels: diffusional properties and residual alignment by mechanical stress or embedding of oriented purple membranes.

Authors: H J Sass; G Musco; S J Stahl; P T Wingfield; S Grzesiek
Journal: J Biomol NMR Date: 2000-12 Impact factor: 2.835

3. An easy way to include weak alignment constraints into NMR structure calculations.

Authors: H J Sass; G Musco; S J Stahl; P T Wingfield; S Grzesiek
Journal: J Biomol NMR Date: 2001-11 Impact factor: 2.835

4. Structure refinement of flexible proteins using dipolar couplings: application to the protein p8MTCP1.

Authors: Hélène Déméné; Thierry Ducat; Philippe Barthe; Marc-André Delsuc; Christian Roumestand
Journal: J Biomol NMR Date: 2002-01 Impact factor: 2.835

5. Quaternary structure built from subunits combining NMR and small-angle x-ray scattering data.

Authors: Maija-Liisa Mattinen; Kimmo Pääkkönen; Teemu Ikonen; Jeremy Craven; Torbjörn Drakenberg; Ritva Serimaa; Jonathan Waltho; Arto Annila
Journal: Biophys J Date: 2002-08 Impact factor: 4.033

6. Model building of a protein-protein complexed structure using saturation transfer and residual dipolar coupling without paired intermolecular NOE.

Authors: Tomoki Matsuda; Takahisa Ikegami; Nobuyuki Nakajima; Toshio Yamazaki; Haruki Nakamura
Journal: J Biomol NMR Date: 2004-07 Impact factor: 2.835