Literature DB >> 7312623

Identification and suppression of secondary structures formed from deoxy-oligonucleotides during electrophoresis in denaturing polyacrylamide-gels.

R Frank, D Müller, C Wolff.   

Abstract

Nature and stability of secondary structures formed from DNA-fragments during electrophoresis in urea containing polyacrylamide-gels were investigated. Duplices and especially hairpin loops were found to be surprisingly stable. Polyacrylamide-gels containing high concentrations of formamide effectively melt these structures. Such gels will improve nucleotide sequence analysis in regions of dyad symmetry that often cause 'band compressions' under standard electrophoresis conditions.

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Year:  1981        PMID： 7312623      PMCID： PMC327492          DOI： 10.1093/nar/9.19.4967

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.

Authors:  F Sanger; A R Coulson
Journal:  J Mol Biol       Date:  1975-05-25       Impact factor: 5.469

2.  RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

Authors:  H Lehrach; D Diamond; J M Wozney; H Boedtker
Journal:  Biochemistry       Date:  1977-10-18       Impact factor: 3.162

3.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

4.  RNA sequencing with radioactive chain-terminating ribonucleotides.

Authors:  F R Kramer; D R Mills
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

5.  T4 polynucleotide ligase catalyzed joining of short synthetic DNA duplexes at base-paired ends.

Authors:  K V Deugau; J H van de Sande
Journal:  Biochemistry       Date:  1978-02-21       Impact factor: 3.162

6.  The use of thin acrylamide gels for DNA sequencing.

Authors:  F Sanger; A R Coulson
Journal:  FEBS Lett       Date:  1978-03-01       Impact factor: 4.124

7.  Structure-independent nucleotide sequence analysis.

Authors:  D R Mills; F R Kramer
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels.

Authors:  R Frank; H Köster
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971
  9 in total
  11 in total

1.  A maturase-like coding sequence downstream of the OXI2 gene of yeast mitochondrial DNA is interrupted by two GC clusters and a putative end-of-messenger signal.

Authors:  F Michel
Journal:  Curr Genet       Date:  1984-05       Impact factor: 3.886

2.  Evolutionary appearance of genes encoding proteins associated with box H/ACA snoRNAs: cbf5p in Euglena gracilis, an early diverging eukaryote, and candidate Gar1p and Nop10p homologs in archaebacteria.

Authors:  Y Watanabe; M W Gray
Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

3.  A rapid method for analyzing the ligation products of synthetic oligodeoxyribonucleotides.

Authors:  L U Tan; B R Glick
Journal:  Mol Biol Rep       Date:  1987       Impact factor: 2.316

4.  Isolation of cDNA clones encoding protein kinase C: evidence for a protein kinase C-related gene family.

Authors:  G M Housey; C A O'Brian; M D Johnson; P Kirschmeier; I B Weinstein
Journal:  Proc Natl Acad Sci U S A       Date:  1987-02       Impact factor: 11.205

5.  Sequence specificity of exonuclease III from E. coli.

Authors:  W Linxweiler; W Hörz
Journal:  Nucleic Acids Res       Date:  1982-08-25       Impact factor: 16.971

6.  Structure and expression of the overlapping ND4L and ND5 genes of Neurospora crassa mitochondria.

Authors:  M A Nelson; G Macino
Journal:  Mol Gen Genet       Date:  1987-02

7.  A mitochondrial reading frame which may code for a maturase-like protein in Saccharomyces cerevisiae.

Authors:  B Séraphin; M Simon; G Faye
Journal:  Nucleic Acids Res       Date:  1985-04-25       Impact factor: 16.971

8.  The repressor gene (c) of the Streptomyces temperate phage phi c31: nucleotide sequence, analysis and functional cloning.

Authors:  R B Sinclair; M J Bibb
Journal:  Mol Gen Genet       Date:  1988-08

9.  U1 small nuclear RNA and spliceosomal introns in Euglena gracilis.

Authors:  D G Breckenridge; Y Watanabe; S J Greenwood; M W Gray; M N Schnare
Journal:  Proc Natl Acad Sci U S A       Date:  1999-02-02       Impact factor: 11.205

10.  Sequence analysis of 28S ribosomal DNA from the amphibian Xenopus laevis.

Authors:  V C Ware; B W Tague; C G Clark; R L Gourse; R C Brand; S A Gerbi
Journal:  Nucleic Acids Res       Date:  1983-11-25       Impact factor: 16.971
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