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Literature DB >> 461182

DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels.

Abstract

The specific influence of the four nucleobases on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels under denaturing and nondenaturing conditions has been investigated using homooligomers from the four deoxyribonucleotides as chain length standards. Homooligomers of same chain lengths exhibit remarkable differences in mobility. Specific retardation of any other oligonucleotide investigated was found to be mainly dependent on base composition but not on sequence. A simple procedure is presented for calculating mobilities relative to the standards on denaturing gels. This allows a reliable identification of oligonucleotides on acrylamide-gels by exact chain length determination with respect to base composition and furthermore a detailed interpretation of complex reaction mixtures. The homooligomers also show the same differences in mobility on nondenaturing gels. The significance of this effect for strand separation is discussed.

Entities: Chemical Disease

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Year: 1979 PMID： 461182 PMCID： PMC327837 DOI： 10.1093/nar/6.6.2069

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

17 in total

1. THE EFFECT OF UREA, FORMAMIDE, AND GLYCOLS ON THE SECONDARY BINDING FORCES IN THE ION-EXCHANGE CHROMATOGRAPHY OF POLYNUCLEOTIDES OF DEAE-CELLULOSE.

Authors: R V TOMLINSON; G M TENER
Journal: Biochemistry Date: 1963 Jul-Aug Impact factor: 3.162

2. Separation of oligonucleotides, nucleotides, and nucleosides on columns of polystyrene anion-exchangers with solvent systems containing ethanol.

Authors: G T Asteriadis; M A Armbruster; P T Gilham
Journal: Anal Biochem Date: 1976-01 Impact factor: 3.365

3. Preparation of large quantities of separated strands from simian virus 40 DNA restriction fragments by low-temperature low-salt agarose gel electrophoresis.

Authors: D Perlman; J A Huberman
Journal: Anal Biochem Date: 1977-12 Impact factor: 3.365

4. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

Authors: H Lehrach; D Diamond; J M Wozney; H Boedtker
Journal: Biochemistry Date: 1977-10-18 Impact factor: 3.162

5. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

6. Separation of the complementary strands of DNA fragments on polyacrylamide gels.

Authors: A A Szalay; K Grohmann; R L Sinsheimer
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

7. Studies on gene control regions. VI. The 5- methyl of thymine, a lac repressor recognition site.

Authors: D V Goeddel; D G Yansura; M H Caruthers
Journal: Nucleic Acids Res Date: 1977-09 Impact factor: 16.971

8. Studies on gene control regions. 1. Chemical synthesis of lactose operator deoxyribonucleic acid segments.

Authors: D V Goeddel; D G Yansura; M H Caruthers
Journal: Biochemistry Date: 1977-05-03 Impact factor: 3.162

9. Studies on gene control regions. 2. Enzymatic joining of chemically synthesized lactose operator deoxyribonucleic acid segments.

Authors: D G Yansura; D V Goeddel; M H Caruthers
Journal: Biochemistry Date: 1977-05-03 Impact factor: 3.162

10. Polymerization of oligodeoxythymidylates and oligoriboadenylates catalyzed by T4 polynucleotide ligase and their use as analytical markers in polyacrylamide-gel electrophoresis.

Authors: J H van de Sande; B W Kalisch
Journal: Anal Biochem Date: 1976-10 Impact factor: 3.365

28 in total

1. Analysis of short tandem repeat polymorphisms by electrospray ion trap mass spectrometry.

Authors: S Hahner; A Schneider; A Ingendoh; J Mosner
Journal: Nucleic Acids Res Date: 2000-09-15 Impact factor: 16.971

2. Extraordinarily stable mini-hairpins: electrophoretical and thermal properties of the various sequence variants of d(GCGAAAGC) and their effect on DNA sequencing.

Authors: I Hirao; Y Nishimura; Y Tagawa; K Watanabe; K Miura
Journal: Nucleic Acids Res Date: 1992-08-11 Impact factor: 16.971

3. Opening of the extraordinarily stable mini-hairpin d(GCGAAGC).

Authors: B Jollès; M Réfrégiers; A Laigle
Journal: Nucleic Acids Res Date: 1997-11-15 Impact factor: 16.971

4. Spectroscopic and calorimetric investigation on the DNA triplex formed by d(CTCTTCTTTCTTTTCTTTCTTCTC) and d(GAGAAGAAAGA) at acidic pH.

Authors: L E Xodo; G Manzini; F Quadrifoglio
Journal: Nucleic Acids Res Date: 1990-06-25 Impact factor: 16.971

5. Extraordinary stable structure of short single-stranded DNA fragments containing a specific base sequence: d(GCGAAAGC).

Authors: I Hirao; Y Nishimura; T Naraoka; K Watanabe; Y Arata; K Miura
Journal: Nucleic Acids Res Date: 1989-03-25 Impact factor: 16.971

6. Reverse Transcription Past Products of Guanine Oxidation in RNA Leads to Insertion of A and C opposite 8-Oxo-7,8-dihydroguanine and A and G opposite 5-Guanidinohydantoin and Spiroiminodihydantoin Diastereomers.

Authors: Anton Alenko; Aaron M Fleming; Cynthia J Burrows
Journal: Biochemistry Date: 2017-09-11 Impact factor: 3.162

7. Modification of guanine bases by nucleoside phosphoramidite reagents during the solid phase synthesis of oligonucleotides.

Authors: R T Pon; M J Damha; K K Ogilvie
Journal: Nucleic Acids Res Date: 1985-09-25 Impact factor: 16.971