Literature DB >> 3431544

A rapid method for analyzing the ligation products of synthetic oligodeoxyribonucleotides.

L U Tan1, B R Glick.   

Abstract

A method is described for the rapid analysis of DNA ligation products in the assembly of synthetic genes and gene fragments. The method is based on the simultaneous analysis of multiple ligation reactions where a single but different DNA oligomer is radiolabelled per ligation reaction. After each ligation the reaction mixture is electrophoresed on a denaturing, as well as a non-denaturing, polyacrylamide gel allowing one to monitor the ligation reaction products. In addition, a unique method for generating single stranded DNA sizing standards up to approximately 300 nucleotides in length is described.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3431544     DOI： 10.1007/BF00444681

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  9 in total

1.  CXII. Total synthesis of the structural gene for an alanine transfer RNA from yeast. Enzymic joining of the chemically synthesized polydeoxynucleotides to form the DNA duplex representing nucleotide sequence 1 to 20.

Authors:  V Sgaramella; H G Khorana
Journal:  J Mol Biol       Date:  1972-12-28       Impact factor: 5.469

2.  Chemical synthesis and cloning of a tyrosine tRNA gene.

Authors:  E L Brown; R Belagaje; M J Ryan; H G Khorana
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

3.  An efficient method for the sequence analysis of oligodeoxyribonucleotides.

Authors:  A M Banaszuk; K V Deugau; J Sherwood; M Michalak; B R Glick
Journal:  Anal Biochem       Date:  1983-02-01       Impact factor: 3.365

4.  Structure, dynamics, and energetics of deoxyguanosine . thymidine wobble base pair formation in the self-complementary d(CGTGAATTCGCG) duplex in solution.

Authors:  D J Patel; S A Kozlowski; L A Marky; J A Rice; C Broka; J Dallas; K Itakura; K J Breslauer
Journal:  Biochemistry       Date:  1982-02-02       Impact factor: 3.162

5.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from Escherichia coli. 9. Enzymatic joining of chemically synthesized deoxyribopolynucleotide segments corresponding to nucleotide sequence 57-94.

Authors:  A Panet; R Kleppe; K Kleppe; H G Khorana
Journal:  J Biol Chem       Date:  1976-02-10       Impact factor: 5.157

7.  Identification and suppression of secondary structures formed from deoxy-oligonucleotides during electrophoresis in denaturing polyacrylamide-gels.

Authors:  R Frank; D Müller; C Wolff
Journal:  Nucleic Acids Res       Date:  1981-10-10       Impact factor: 16.971

8.  DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels.

Authors:  R Frank; H Köster
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971

9.  Expression in Escherichia coli of chemically synthesized gene for the human immune interferon.

Authors:  S Tanaka; T Oshima; K Ohsuye; T Ono; A Mizono; A Ueno; H Nakazato; M Tsujimoto; N Higashi; T Noguchi
Journal:  Nucleic Acids Res       Date:  1983-03-25       Impact factor: 16.971
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.