Literature DB >> 287063

Structure-independent nucleotide sequence analysis.

D R Mills, F R Kramer.   

Abstract

Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.

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Year:  1979        PMID： 287063      PMCID： PMC383572          DOI： 10.1073/pnas.76.5.2232

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  33 in total

1.  HELIX--RANDOM COIL TRANSITIONS IN DNA HOMOPOLYMER PAIRS.

Authors:  R B INMAN; R L BALDWIN
Journal:  J Mol Biol       Date:  1964-04       Impact factor: 5.469

2.  The role of deoxyribonucleic acid in ribonucleic acid synthesis. IV. The incorporation of pyrimidine and purine analogues into ribonucleic acid.

Authors:  F M KAHAN; J HURWITZ
Journal:  J Biol Chem       Date:  1962-12       Impact factor: 5.157

3.  Tautomeric forms in a polynucleotide helix and their bearing on the structure of DNA.

Authors:  H T MILES
Journal:  Proc Natl Acad Sci U S A       Date:  1961-06-15       Impact factor: 11.205

4.  ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. III. THE INCORPORATION OF PYRIMIDINE AND PURINE ANALOGUES INTO DEOXYRIBONUCLEIC ACID.

Authors:  M J Bessman; I R Lehman; J Adler; S B Zimmerman; E S Simms; A Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1958-07-15       Impact factor: 11.205

5.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

6.  RNA sequencing with radioactive chain-terminating ribonucleotides.

Authors:  F R Kramer; D R Mills
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

7.  Specific termination of RNA polymerase synthesis as a method of RNA and DNA sequencing.

Authors:  V D Axelrod; R M Vartikyan; V A Aivazashvili; R S Beabealashvili
Journal:  Nucleic Acids Res       Date:  1978-10       Impact factor: 16.971

8.  Mapping adenines, guanines, and pyrimidines in RNA.

Authors:  H Donis-Keller; A M Maxam; W Gilbert
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  CONVERSION OF MONO- AND OLIGODEOXYRIBONUCLEOTIDES TO 5-TRIPHOSPHATES.

Authors:  D E HOARD; D G OTT
Journal:  J Am Chem Soc       Date:  1965-04-20       Impact factor: 15.419
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  96 in total

1.  The 2-amino group of guanine is absolutely required for specific binding of the anti-cancer antibiotic echinomycin to DNA.

Authors:  C Marchand; C Bailly; M J McLean; S E Moroney; M J Waring
Journal:  Nucleic Acids Res       Date:  1992-11-11       Impact factor: 16.971

2.  Structural, molecular, and genetic analysis of the kilA operon of broad-host-range plasmid RK2.

Authors:  P Goncharoff; S Saadi; C H Chang; L H Saltman; D H Figurski
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

3.  Use of electrophoretic mobility to determine the secondary structure of a small antisense RNA.

Authors:  J P Jacques; M M Susskind
Journal:  Nucleic Acids Res       Date:  1991-06-11       Impact factor: 16.971

4.  Development of a DNA probe from the deoxyribonucleotide sequence of a 3-N-aminoglycoside acetyltransferase [AAC(3)-I] resistance gene.

Authors:  F C Tenover; K L Phillips; T Gilbert; P Lockhart; P J O'Hara; J J Plorde
Journal:  Antimicrob Agents Chemother       Date:  1989-04       Impact factor: 5.191

5.  Sequence requirements of Escherichia coli attTn7, a specific site of transposon Tn7 insertion.

Authors:  R L McKown; K A Orle; T Chen; N L Craig
Journal:  J Bacteriol       Date:  1988-01       Impact factor: 3.490

6.  RNA sequencing using fluorescent-labeled dideoxynucleotides and automated fluorescence detection.

Authors:  G J Bauer
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

7.  Binding of Epstein-Barr virus small RNA EBER-1 to the double-stranded RNA-activated protein kinase DAI.

Authors:  P A Clarke; M Schwemmle; J Schickinger; K Hilse; M J Clemens
Journal:  Nucleic Acids Res       Date:  1991-01-25       Impact factor: 16.971

8.  Comparative analysis of the replication regions of IncB, IncK, and IncZ plasmids.

Authors:  J Praszkier; T Wei; K Siemering; J Pittard
Journal:  J Bacteriol       Date:  1991-04       Impact factor: 3.490

9.  NADH:ubiquinone oxidoreductase from bovine heart mitochondria. cDNA sequences of the import precursors of the nuclear-encoded 39 kDa and 42 kDa subunits.

Authors:  I M Fearnley; M Finel; J M Skehel; J E Walker
Journal:  Biochem J       Date:  1991-09-15       Impact factor: 3.857

10.  The antigen of hepatitis delta virus: examination of in vitro RNA-binding specificity.

Authors:  M Chao; S Y Hsieh; J Taylor
Journal:  J Virol       Date:  1991-08       Impact factor: 5.103
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