Literature DB >> 7177845

A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of lambda cro binding heptadecanucleotide on a polymer support.

E Ohtsuka, Z Tozuka, S Iwai, M Ikehara.   

Abstract

A new condensing reagent 1-(2,4,6,-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide (TPSPy) was found to give one diastereoisomer of dinucleoside monophosphate aryl esters. Several oligodeoxynucleotide blocks were prepared using this reagent. A heptadecanucleotide, dTATCCCTTGCGGTGATA, which had the same sequence as the lambda cro binding DNA sequence was synthesized by condensing mono-, tri- and dodecanucleotide blocks using this reagent on a polystyrene support.

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Year:  1982        PMID： 7177845      PMCID： PMC326913          DOI： 10.1093/nar/10.20.6235

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

Review 1.  How the lambda repressor and cro work.

Authors:  M Ptashne; A Jeffrey; A D Johnson; R Maurer; B J Meyer; C O Pabo; T M Roberts; R T Sauer
Journal:  Cell       Date:  1980-01       Impact factor: 41.582

2.  High-pressure liquid chromatography in polynucleotide synthesis.

Authors:  H J Fritz; R Belagaje; E L Brown; R H Fritz; R A Jones; R G Lees; H G Khorana
Journal:  Biochemistry       Date:  1978-04-04       Impact factor: 3.162

3.  Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent.

Authors:  E Ohtsuka; Y Taniyama; R Marumoto; H Sato; H Hirosaki; M Ikehara
Journal:  Nucleic Acids Res       Date:  1982-04-24       Impact factor: 16.971

4.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

5.  Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.

Authors:  H Ito; Y Ike; S Ikuta; K Itakura
Journal:  Nucleic Acids Res       Date:  1982-03-11       Impact factor: 16.971

6.  Simplications in the synthesis of short oligonucleotide blocks.

Authors:  C Broka; T Hozumi; R Arentzen; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

7.  Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.

Authors:  K Miyoshi; T Miyake; T Hozumi; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971
  7 in total
  3 in total

1.  Synthesis and applications of oligoribonucleotides with selected 2'-O-methylation using the 2'-O-[1-(2-fluorophenyl)-4-methoxypiperidin-4-yl] protecting group.

Authors:  B Beijer; I Sulston; B S Sproat; P Rider; A I Lamond; P Neuner
Journal:  Nucleic Acids Res       Date:  1990-09-11       Impact factor: 16.971

2.  Highly efficient chemical synthesis of 2'-O-methyloligoribonucleotides and tetrabiotinylated derivatives; novel probes that are resistant to degradation by RNA or DNA specific nucleases.

Authors:  B S Sproat; A I Lamond; B Beijer; P Neuner; U Ryder
Journal:  Nucleic Acids Res       Date:  1989-05-11       Impact factor: 16.971

3.  An approach to the stereoselective synthesis of Sp-dinucleoside phosphorothioates using phosphotriester chemistry.

Authors:  R Cosstick; D M Williams
Journal:  Nucleic Acids Res       Date:  1987-12-10       Impact factor: 16.971
  3 in total

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