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Literature DB >> 7133994

Ultrafast sequencing of oligodeoxyribonucleotides by FAB-mass spectrometry.

Abstract

A fully instrumental method is described for the bidirectional sequencing of oligodeoxyribonucleotides. The method makes use of the negative ion fragmentation patterns of fast atom bombardment mass spectrometry. It is less time consuming than any other sequencing procedure known to date. Since one sequencing run takes as little as one hour, this new method is anticipated to cut down considerably the time required for the controlled synthesis of oligodeoxyribonucleotides of (currently) up to ten nucleotide units in length.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1982 PMID： 7133994 PMCID： PMC321120 DOI： 10.1093/nar/10.15.4671

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

7 in total

1. Letter: Phosphite coupling procedure for generating internucleotide links.

Authors: R I Letsinger; J L Finnan; G A Heavner; N B Lunsford
Journal: J Am Chem Soc Date: 1975-05-28 Impact factor: 15.419

2. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

3. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.

Authors: G G Brownlee; F Sanger
Journal: Eur J Biochem Date: 1969-12

4. Extended performance using a high field magnet mass spectrometer.

Authors: H R Morris; A Dell; R A McDowell
Journal: Biomed Mass Spectrom Date: 1981-09

5. Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.

Authors: H Ito; Y Ike; S Ikuta; K Itakura
Journal: Nucleic Acids Res Date: 1982-03-11 Impact factor: 16.971

6. DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels.

Authors: R Frank; H Köster
Journal: Nucleic Acids Res Date: 1979 Impact factor: 16.971

7. Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.

Authors: M L Duckworth; M J Gait; P Goelet; G F Hong; M Singh; R C Titmas
Journal: Nucleic Acids Res Date: 1981-04-10 Impact factor: 16.971

7 in total

18 in total

1. RNA fragmentation in MALDI mass spectrometry studied by H/D-exchange: mechanisms of general applicability to nucleic acids.

Authors: Thomas E Andersen; Finn Kirpekar; Kim F Haselmann
Journal: J Am Soc Mass Spectrom Date: 2006-07-27 Impact factor: 3.109

2. Continuous-flow fast atom bombardment mass spectrometry of oligonucleotides.

Authors: R B van Breemen; L B Martin; J C Le
Journal: J Am Soc Mass Spectrom Date: 1991-04 Impact factor: 3.109

3. Investigations of the metastable decay of DNA under ultraviolet matrix-assisted laser desorption/ionization conditions with post-source-decay analysis and hydrogen/deuterium exchange.

Authors: J Gross; A Leisner; F Hillenkamp; S Hahner; M Karas; J Schäfer; F Lützenkirchen; E Nordhoff
Journal: J Am Soc Mass Spectrom Date: 1998-09 Impact factor: 3.109

10. Structure and fragmentation mechanisms of isomeric T-rich oligodeoxynucleotides: a comparison of four tandem mass spectrometric methods.

Authors: Z Wang; K X Wan; R Ramanathan; J S Taylor; M L Gross
Journal: J Am Soc Mass Spectrom Date: 1998-07 Impact factor: 3.109

Ultrafast sequencing of oligodeoxyribonucleotides by FAB-mass spectrometry.

1. Letter: Phosphite coupling procedure for generating internucleotide links.

2. A new method for sequencing DNA.

3. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.

4. Extended performance using a high field magnet mass spectrometer.

5. Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.

6. DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels.

7. Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.

1. RNA fragmentation in MALDI mass spectrometry studied by H/D-exchange: mechanisms of general applicability to nucleic acids.

2. Continuous-flow fast atom bombardment mass spectrometry of oligonucleotides.

3. Investigations of the metastable decay of DNA under ultraviolet matrix-assisted laser desorption/ionization conditions with post-source-decay analysis and hydrogen/deuterium exchange.

Review 4. Fast atom bombardment mass spectrometry and its application to the analysis of some peptides and proteins.

5. Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases.

Review 6. Mass spectrometry of structurally modified DNA.

7. Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides.

Review 8. Tandem mass spectrometry for characterization of covalent adducts of DNA with anticancer therapeutics.

9. Color coded triarylmethyl protecting groups useful for deoxypolynucleotide synthesis.

10. Structure and fragmentation mechanisms of isomeric T-rich oligodeoxynucleotides: a comparison of four tandem mass spectrometric methods.