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Literature DB >> 8041606

Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides.

E Nordhoff¹, F Kirpekar, M Karas, R Cramer, S Hahner, F Hillenkamp, K Kristiansen, P Roepstroff, A Lezius.

Abstract

UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1994 PMID： 8041606 PMCID： PMC308196 DOI： 10.1093/nar/22.13.2460

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

1. Matrix-assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared.

Authors: E Nordhoff; A Ingendoh; R Cramer; A Overberg; B Stahl; M Karas; F Hillenkamp; P F Crain
Journal: Rapid Commun Mass Spectrom Date: 1992-12 Impact factor: 2.419

2. Mass spectrometry of DNA mixtures by laser ablation from frozen aqueous solution.

Authors: D M Schieltz; C W Chou; C W Luo; R M Thomas; P Williams
Journal: Rapid Commun Mass Spectrom Date: 1992-10 Impact factor: 2.419

3. Molecular weight determination of underivatized oligodeoxyribonucleotides by positive-ion matrix-assisted ultraviolet laser-desorption mass spectrometry.

Authors: B Spengler; Y Pan; R J Cotter; L S Kan
Journal: Rapid Commun Mass Spectrom Date: 1990-04 Impact factor: 2.419

Review 4. New developments in biochemical mass spectrometry: electrospray ionization.

Authors: R D Smith; J A Loo; C G Edmonds; C J Barinaga; H R Udseth
Journal: Anal Chem Date: 1990-05-01 Impact factor: 6.986

5. Antisense DNA oligonucleotides. II: The use of matrix-assisted laser desorption/ionization mass spectrometry for the sequence verification of methylphosphonate oligodeoxyribonucleotides.

Authors: T Keough; T R Baker; R L Dobson; M P Lacey; T A Riley; J A Hasselfield; P E Hesselberth
Journal: Rapid Commun Mass Spectrom Date: 1993-03 Impact factor: 2.419

6. Ultrafast sequencing of oligodeoxyribonucleotides by FAB-mass spectrometry.

Authors: L Grotjahn; R Frank; H Blöcker
Journal: Nucleic Acids Res Date: 1982-08-11 Impact factor: 16.971

7. Matrix-assisted laser desorption mass spectrometry of oligodeoxythymidylic acids.

Authors: T Huth-Fehre; J N Gosine; K J Wu; C H Becker
Journal: Rapid Commun Mass Spectrom Date: 1992-03 Impact factor: 2.419

8. Basic matrices for the matrix-assisted laser desorption/ionization mass spectrometry of proteins and oligonucleotides.

Authors: M C Fitzgerald; G R Parr; L M Smith
Journal: Anal Chem Date: 1993-11-15 Impact factor: 6.986

9. Matrix-assisted laser desorption time-of-flight mass spectrometry of oligonucleotides using 3-hydroxypicolinic acid as an ultraviolet-sensitive matrix.

Authors: K J Wu; A Steding; C H Becker
Journal: Rapid Commun Mass Spectrom Date: 1993-02 Impact factor: 2.419

10. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

19 in total

1. Fragmentation pathway studies of oligonucleotides in matrix-assisted laser desorption/ionization mass spectrometry by charge tagging and H/D exchange.

Authors: Chau-Wen Chou; Patrick A Limbach; Richard B Cole
Journal: J Am Soc Mass Spectrom Date: 2002-12 Impact factor: 3.109

2. RNaseCut: a MALDI mass spectrometry-based method for SNP discovery.

Authors: Stefan Krebs; Ivica Medugorac; Doris Seichter; Martin Förster
Journal: Nucleic Acids Res Date: 2003-04-01 Impact factor: 16.971

3. Rapid and accurate characterisation of short tandem repeats by MALDI-TOF analysis of endonuclease cleaved RNA transcripts.

Authors: Doris Seichter; Stefan Krebs; Martin Förster
Journal: Nucleic Acids Res Date: 2004-01-20 Impact factor: 16.971

4. RNA fragmentation in MALDI mass spectrometry studied by H/D-exchange: mechanisms of general applicability to nucleic acids.

Authors: Thomas E Andersen; Finn Kirpekar; Kim F Haselmann
Journal: J Am Soc Mass Spectrom Date: 2006-07-27 Impact factor: 3.109

5. Direct comparison of infrared and ultraviolet wavelength matrix-assisted laser desorption/ionization mass spectrometry of proteins.

Authors: S Niu; W Zhang; B T Chait
Journal: J Am Soc Mass Spectrom Date: 1998-01 Impact factor: 3.109

6. Investigations of the metastable decay of DNA under ultraviolet matrix-assisted laser desorption/ionization conditions with post-source-decay analysis and hydrogen/deuterium exchange.

Authors: J Gross; A Leisner; F Hillenkamp; S Hahner; M Karas; J Schäfer; F Lützenkirchen; E Nordhoff
Journal: J Am Soc Mass Spectrom Date: 1998-09 Impact factor: 3.109