Literature DB >> 202925

The use of nuclease P1 in sequence analysis of end group labeled RNA.

M Silberklang, A M Gillum, U L RajBhandary.   

Abstract

A method is described for the direct sequence analysis of 20-25 nucleotides from the termini of 5'- or 3'-end-group [32P] labeled RNA. The method involves partial endonucleolytic digestion of the labeled RNA with nuclease P1 (from Penicillium citrinum) followed by separation of the partial digestion products by two-dimensional homochromatography, the nucleotide sequence being determined by mobility shift analysis. This procedure has been applied to the sequence analysis of the terminal regions of tRNAs and of high molecular weight RNA, such as messenger RNA or viral RNA. A further application involves its use in conjunction with snake venom phosphodiesterase to determine the sequence of 5'-end group labeled oligonucleotides, containing modified bases, derived from T1 or pancreatic RNase digestion of tRNA.

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Year:  1977        PMID： 202925      PMCID： PMC343228          DOI： 10.1093/nar/4.12.4091

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  Capping of eucaryotic mRNAs.

Authors:  A J Shatkin
Journal:  Cell       Date:  1976-12       Impact factor: 41.582

2.  Nucleotide sequence of salmon testes and salmon liver cytoplasmic initiator tRNA.

Authors:  A M Gillum; N Urquhart; M Smith; U L RajBhandary
Journal:  Cell       Date:  1975-11       Impact factor: 41.582

3.  Tumorigenic action of streptozotocin on the pancreas and kidney in male Wistar rats.

Authors:  T Kazumi; G Yoshino; S Fujii; S Baba
Journal:  Cancer Res       Date:  1978-07       Impact factor: 12.701

4.  Nucleotide sequences at the 5'termini of rabbit alpha and beta globin mRNA.

Authors:  R E Lockhard; U L Rajbhandary
Journal:  Cell       Date:  1976-12       Impact factor: 41.582

5.  Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.

Authors:  A Panet; J H van de Sande; P C Loewen; H G Khorana; A J Raae; J R Lillehaug; K Kleppe
Journal:  Biochemistry       Date:  1973-12-04       Impact factor: 3.162

Review 6.  The primary structure of DNA.

Authors:  K Murray; R W Old
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1974

7.  The first nucleotide sequence of an organelle transfer RNA: chloroplastic tRNAphe.

Authors:  S H Chang; C K Brum; M Siberklang; U L RajBhandary; L I Hecker; W E Barnett
Journal:  Cell       Date:  1976-12       Impact factor: 41.582

8.  Studies on the sequence of the 3'-terminal region of turnip-yellow-mosaic-virus RNA.

Authors:  M Silberklang; A Prochiantz; A L Haenni; U L Rajbhandary
Journal:  Eur J Biochem       Date:  1977-02

9.  Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

Authors:  A C Peacock; C W Dingman
Journal:  Biochemistry       Date:  1967-06       Impact factor: 3.162

10.  Nucleotide sequence of human placenta cytoplasmic initiator tRNA.

Authors:  A M Gillum; B A Roe; M P Anandaraj; U L RajBhandary
Journal:  Cell       Date:  1975-11       Impact factor: 41.582
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  87 in total

1.  Two-piece tmRNA in cyanobacteria and its structural analysis.

Authors:  Cyril Gaudin; Xiong Zhou; Kelly P Williams; Brice Felden
Journal:  Nucleic Acids Res       Date:  2002-05-01       Impact factor: 16.971

2.  The nucleotide sequence of the initiator tRNA from Drosophila melanogaster.

Authors:  S Silverman; J Heckman; G J Cowling; A D Delaney; R J Dunn; I C Gillam; G M Tener; D Söll; U L RajBhandary
Journal:  Nucleic Acids Res       Date:  1979-02       Impact factor: 16.971

3.  Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA.

Authors:  H Guilley; G Jonard; B Kukla; K E Richards
Journal:  Nucleic Acids Res       Date:  1979-04       Impact factor: 16.971

4.  Conformational change of Escherichia coli initiator methionyl-tRNA(fMet) upon binding to methionyl-tRNA formyl transferase.

Authors:  Christine Mayer; Uttam L RajBhandary
Journal:  Nucleic Acids Res       Date:  2002-07-01       Impact factor: 16.971

5.  Structural specificity of Rn nuclease I as probed on yeast tRNA(Phe) and tRNA(Asp).

Authors:  A Przykorska; C el Adlouni; G Keith; J W Szarkowski; G Dirheimer
Journal:  Nucleic Acids Res       Date:  1992-02-25       Impact factor: 16.971

6.  Atypical archaeal tRNA pyrrolysine transcript behaves towards EF-Tu as a typical elongator tRNA.

Authors:  Anne Théobald-Dietrich; Magali Frugier; Richard Giegé; Joëlle Rudinger-Thirion
Journal:  Nucleic Acids Res       Date:  2004-02-10       Impact factor: 16.971

7.  Nucleotide sequence at the 5' extremity of turnip yellow mosaic virus genome RNA.

Authors:  J P Briand; G Keith; H Guilley
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

8.  The nucleotide sequence of spinach chloroplast tryptophan transfer RNA.

Authors:  J Canaday; P Guillemaut; R Gloeckler; J H Weil
Journal:  Nucleic Acids Res       Date:  1981-01-10       Impact factor: 16.971

9.  Chemical conversion of cytidine residues into 4-thiouridines in yeast tRNAPhe. Determination of the modified cytidines.

Authors:  N Riehl; P Carbon; B Ehresmann; J P Ebel
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

10.  Relationship between promoter structure and template specificities exhibited by the bacteriophage T3 and T7 RNA polymerases.

Authors:  J N Bailey; J F Klement; W T McAllister
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205
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