Literature DB >> 7016910

Comparison of hemagglutination inhibition test and enzyme-linked immunosorbent assay for determining antibody to rubella virus.

I C Shekarchi, J L Sever, N Tzan, A Ley, L C Ward, D Madden.   

Abstract

The hemagglutination inhibition test (HAI) and the enzyme-linked immunosorbent assay (ELISA) for detecting antibody to rubella virus were compared by testing 25 sets of paired sera taken before and after infection and 10 sets of sera taken during acute and convalescent stages of the disease and by screening 700 serum samples from the Collaborative Perinatal Project, NIH/NINCDS. The tests were found to be comparable in their ability to detect positive and negative sera, rises in titers, and seroconversions. When a purified antigen and carefully prepared reagents were used, ELISA was found to be as accurate and reliable as HAI. ELISA required no pretreatment of serum, could easily be automated, and was less time-consuming than HAI.

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Year:  1981        PMID： 7016910      PMCID： PMC273902          DOI： 10.1128/jcm.13.5.850-854.1981

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Application of a microtechnique to viral serological investigations.

Authors:  J L SEVER
Journal:  J Immunol       Date:  1962-03       Impact factor: 5.422

2.  Center for Disease Control Diagnostic Immunology Proficiency Testing Program results for 1977.

Authors:  R N Taylor; K M Fulford; V A Przybyszewski; V Pope
Journal:  J Clin Microbiol       Date:  1978-10       Impact factor: 5.948

3.  Center for disease control diagnostic immunology proficiency testing program results for 1976.

Authors:  R N Taylor; K M Fulford; A Przybyszewski; V Pope
Journal:  J Clin Microbiol       Date:  1977-09       Impact factor: 5.948

4.  Center for Disease Control Diagnostic Immunology Proficiency Testing Program results for 1978.

Authors:  R N Taylor; K M Fulford; V A Przybyszewski; V Pope
Journal:  J Clin Microbiol       Date:  1979-12       Impact factor: 5.948

5.  Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies.

Authors:  K O Kalimo; O H Meurman; P E Halonen; B R Ziola; M K Viljanen; K Granfors; P Toivanen
Journal:  J Clin Microbiol       Date:  1976-08       Impact factor: 5.948

6.  A solid-phase enzyme-linked immunosorbent assay (ELISA) for detection of antibody to rubella virus.

Authors:  S M Garland; S A Locarnini; I D Gust
Journal:  Pathology       Date:  1979-07       Impact factor: 5.306

7.  Rubella in the collaborative perinatal research study. II. Clinical and laboratory findings in children through 3 years of age.

Authors:  J L Sever; J B Hardy; K B Nelson; M R Gilkeson
Journal:  Am J Dis Child       Date:  1969-07

8.  Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin.

Authors:  P Leinikki; S Pässilä
Journal:  J Clin Pathol       Date:  1976-12       Impact factor: 3.411

9.  Antigen requirements, sensitivity, and specificity of enzyme immunoassays for measles and rubella viral antibodies.

Authors:  B Forghani; N J Schmidt
Journal:  J Clin Microbiol       Date:  1979-06       Impact factor: 5.948

10.  Rubella serology: a need for improvement.

Authors:  J L Sever
Journal:  Obstet Gynecol       Date:  1980-07       Impact factor: 7.661
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  11 in total

1.  Comparison of enzyme-linked immunosorbent assay, hemagglutination inhibition, and passive latex agglutination for determination of rubella immune status.

Authors:  R S Steece; M S Talley; M R Skeels; G A Lanier
Journal:  J Clin Microbiol       Date:  1985-01       Impact factor: 5.948

2.  Comparison of an enzyme-linked immunosorbent assay with indirect hemagglutination and hemagglutination inhibition for determination of rubella virus antibody: evaluation of immune status with commercial reagents in a clinical laboratory.

Authors:  A L Truant; B L Barksdale; T W Huber; L B Elliott
Journal:  J Clin Microbiol       Date:  1983-01       Impact factor: 5.948

3.  Problems in determining immune status in borderline specimens in an enzyme immunoassay for rubella immunoglobulin G antibody.

Authors:  R S Steece; M S Talley; M R Skeels; G A Lanier
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948

4.  Evaluation of a latex agglutination test for detection of antibodies to rubella virus in selected sera.

Authors:  S Freeman; L Clark; N Dumas
Journal:  J Clin Microbiol       Date:  1983-07       Impact factor: 5.948

5.  Comparison of the latex agglutination test with the hemagglutination inhibition test, enzyme-linked immunosorbent assay, and neutralization test for detection of antibodies to rubella virus.

Authors:  J M Meegan; B K Evans; D M Horstmann
Journal:  J Clin Microbiol       Date:  1982-10       Impact factor: 5.948

6.  Determination of immune status in patients with low antibody titers for rubella virus.

Authors:  S L Fayram; S Akin; S L Aarnaes; E M Peterson; L M de la Maza
Journal:  J Clin Microbiol       Date:  1987-01       Impact factor: 5.948

7.  Rapid latex agglutination test for rubella antibody.

Authors:  J L Sever; N R Tzan; I C Shekarchi; D L Madden
Journal:  J Clin Microbiol       Date:  1983-01       Impact factor: 5.948

8.  New passive hemagglutination assay kit that uses hemagglutinin-sensitized erythrocytes for detection of rubella antibodies.

Authors:  S R Coates; R D Madsen; D F Rippe
Journal:  J Clin Microbiol       Date:  1982-12       Impact factor: 5.948

9.  New latex agglutination test for rapid determination of rubella immune status.

Authors:  A S Weissfeld; A C Sonnenwirth
Journal:  J Clin Microbiol       Date:  1982-11       Impact factor: 5.948

10.  Comparison of several test systems used for determination of rubella immune status.

Authors:  A S Weissfeld; W D Gehle; A C Sonnenwirth
Journal:  J Clin Microbiol       Date:  1982-07       Impact factor: 5.948
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