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Literature DB >> 795817

Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin.

Abstract

A solid phase antibody assay by means of alkaline phosphatase conjugated to antiimmunoglobulin is described. Specially designed microcuvettes were sensitized with influenza A antigen, and antibodies bound to it were assayed by anti-IgG alkaline phosphatase conjugate in a semiautomated photometer equipped with a programmable calculator. The sensitivity was found to be 200 times higher than HI- or CF-techniques, and the interassay variation was so small that twofold changes in antobody activity could be regarded as significant. Results from vaccinees indicated that serum samples could be collected at intervals of three to six days only to reach a serological diagnosis in clinical patients.

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Year: 1976 PMID： 795817 PMCID： PMC476314 DOI： 10.1136/jcp.29.12.1116

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

8 in total

1. Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes.

Authors: E Engvall; K Jonsson; P Perlmann
Journal: Biochim Biophys Acta Date: 1971-12-28

2. A microplate method of enzyme-linked immunosorbent assay and its application to malaria.

Authors: A Voller; D Bidwell; G Huldt; E Engvall
Journal: Bull World Health Organ Date: 1974 Impact factor: 9.408

3. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors: E Engvall; P Perlmann
Journal: J Immunol Date: 1972-07 Impact factor: 5.422

4. Titration of antibodies to salmonella O antigens by enzyme-linked immunosorbent assay.

Authors: H E Carlsson; A A Lindberg; S Hammarström
Journal: Infect Immun Date: 1972-11 Impact factor: 3.441

5. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.

Authors: S Avrameas
Journal: Immunochemistry Date: 1969-01

6. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.

Authors: E Engvall; P Perlmann
Journal: Immunochemistry Date: 1971-09

7. Enzyme-linked immunosorbent assays for cholera serology.

Authors: J Holmgren; A M Svennerholm
Journal: Infect Immun Date: 1973-05 Impact factor: 3.441

8. A simple method for detecting antibodies to rubella.

Authors: A Voller; D E Bidwell
Journal: Br J Exp Pathol Date: 1975-08

8 in total

21 in total

1. Rapid detection of IgG and IgM antibodies for cytomegalovirus by the enzyme linked immunosorbent assay (ELISA).

Authors: R Cappel; F de Cuyper; J de Braekeleer
Journal: Arch Virol Date: 1978 Impact factor: 2.574

2. Enzyme-linked immunosorbent assay for detection of antibodies to influenza A and B and parainfluenza type 1 in sera of patients.

Authors: F R Bishai; R Galli
Journal: J Clin Microbiol Date: 1978-12 Impact factor: 5.948

3. Enzyme-linked immunosorbent assay determination of specific rubella antibody levels in micrograms of immunoglobulin G per milliliter of serum in clinical samples.

Authors: P O Leinikki; I Shekarchi; P Dorsett; J L Sever
Journal: J Clin Microbiol Date: 1978-10 Impact factor: 5.948

4. Antibody to Herpesvirus hominis in patients with carcinoma of the cervix.

Authors: S N Najem; I G Barton; L S Al-Omar; C W Potter
Journal: Br J Exp Pathol Date: 1982-10

5. Enzyme-linked immunosorbent assay for quantitation of toxoplasma antibodies in human sera.

Authors: A M van Loon; J van der Veen
Journal: J Clin Pathol Date: 1980-07 Impact factor: 3.411

6. Comparison of ELISA and RIA for measurement of pneumococcal antibodies before and after vaccination with 14-valent pneumococcal capsular polysaccharide vaccine.

Authors: M Koskela; M Leinonen
Journal: J Clin Pathol Date: 1981-01 Impact factor: 3.411

7. Multiple sclerosis in the Orkney and Shetland Islands. IV: Viral antibody titres and viral infections.

Authors: D C Poskanzer; J L Sever; J L Sheridan; L B Prenney
Journal: J Epidemiol Community Health Date: 1980-12 Impact factor: 3.710

8. Measurement of immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: persistence of serum antibodies during disease.

Authors: K Granfors
Journal: J Clin Microbiol Date: 1979-03 Impact factor: 5.948

9. Immunoglobulin G- and M-specific enzyme-linked immunosorbent assay for detection of dengue antibodies.

Authors: D Dittmar; T J Cleary; A Castro
Journal: J Clin Microbiol Date: 1979-04 Impact factor: 5.948

10. Antigen requirements and specificity of a microplate enzyme-linked immunosorbent assay (ELISA) for detecting infectious bronchitis viral antibodies in chicken serum.

Authors: A Soula; Y Moreau
Journal: Arch Virol Date: 1981 Impact factor: 2.574