Literature DB >> 6777122

The localization of tRNA5Asn, tRNAHis, and tRNAAla genes from drosophila melanogaster by in situ hybridization to polytene salivary gland chromosomes.

T Schmidt, E Kubli.   

Abstract

Transfer RNA5 gammaAsn, tRNA gamma His, and tRNAAla were isolated from Drosophila melanogaster by means of Sepharose 4B chromatography and 2-dimensional polyacrylamide gel electrophoresis. The tRNAs were iodinated in vitro with Na125I and hybridized in situ salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNA 5 gammaAsn in the regions 42A, 59F, 60C, and 84F; for tRNAHis in the regions 48F and 56E; and for tRNAAla in the regions 63A and 90C. From these and our previous results it can be concluded that the genes for the Q-base containing tRNAs (tRNAAsn, tRNAAsp, and tRNAHis, are not clustered in the Drosophila melanogaster genome.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 6777122     DOI： 10.1007/bf00292685

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  27 in total

1.  Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.

Authors:  S Hayashi; I C Gillam; A D Delaney; G M Tener
Journal:  J Histochem Cytochem       Date:  1978-08       Impact factor: 2.479

2.  Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus.

Authors:  B N White; G M Tener
Journal:  J Mol Biol       Date:  1973-03-15       Impact factor: 5.469

3.  Scintillation counting: a comparison of the counting efficiencies of serveral aqueous solubilizers.

Authors:  G W Carter; K V Dyke
Journal:  Anal Biochem       Date:  1973-08       Impact factor: 3.365

4.  Formation and detection of RNA-DNA hybrid molecules in cytological preparations.

Authors:  J G Gall; M L Pardue
Journal:  Proc Natl Acad Sci U S A       Date:  1969-06       Impact factor: 11.205

5.  Analysis of a drosophila tRNA gene cluster.

Authors:  B Hovemann; S Sharp; H Yamada; D Söll
Journal:  Cell       Date:  1980-04       Impact factor: 41.582

6.  Messenger RNA in heat-shocked Drosophila cells.

Authors:  A Spradling; M L Pardue; S Penman
Journal:  J Mol Biol       Date:  1977-02-05       Impact factor: 5.469

7.  The localization of tRNA4Glu genes from Drosophila melanogaster by "in situ" hybridization.

Authors:  E Kubli; T Schmidt
Journal:  Nucleic Acids Res       Date:  1978-05       Impact factor: 16.971

8.  Transfer RNA in aging Drosophila: II. Isoacceptor patterns.

Authors:  H A Hosbach; E Kubli
Journal:  Mech Ageing Dev       Date:  1979-04       Impact factor: 5.432

9.  Transfer RNA genes of Drosophila melanogaster.

Authors:  R Dudler; A H Egg; E Kubli; S Artavanis-Tsakonas; W J Gehring; R Steward; P Schedl
Journal:  Nucleic Acids Res       Date:  1980-07-11       Impact factor: 16.971

10.  The localization of transfer RNA5Lys genes in Drosophila melanogaster.

Authors:  T A Grigliatti; B N White; G M Tener; T C Kaufman; D T Suzuki
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205
View more
  7 in total

Review 1.  The structure and function of tRNA genes of higher eukaryotes.

Authors:  E Kubli
Journal:  Experientia       Date:  1981-01-15

2.  Localization of tRNA genes of Drosophila melanogaster by in situ hybridization.

Authors:  S Hayashi; I C Gillam; T A Grigliatti; G M Tener
Journal:  Chromosoma       Date:  1982       Impact factor: 4.316

3.  Glutamate tRNA genes are adjacent to 5S RNA genes in Drosophila and reveal a conserved upstream sequence (the ACT-TA box).

Authors:  Z K Indik; K D Tartof
Journal:  Nucleic Acids Res       Date:  1982-07-24       Impact factor: 16.971

4.  The genes coding for tRNA Tyr of Drosophila melanogaster: localization of determination of the gene numbers.

Authors:  R Dudler; T Schmidt; M Bienz; E Kubli
Journal:  Chromosoma       Date:  1981       Impact factor: 4.316

5.  The genes coding for 4 snRNAs of Drosophila melanogaster: localization and determination of gene numbers.

Authors:  H P Saluz; T Schmidt; R Dudler; M Altwegg; E Stumm-Zollinger; E Kubli; P S Chen
Journal:  Nucleic Acids Res       Date:  1983-01-11       Impact factor: 16.971

6.  RNA-DNA hybridization analyses of tRNA-Val-3b in Drosophila melanogaster.

Authors:  T M Larsen; R C Miller; G B Spiegelman; S Hayashi; G M Tener; D A Sinclair; T A Grigliatti
Journal:  Mol Gen Genet       Date:  1982

7.  Secrets of secretion-How studies of the Drosophila salivary gland have informed our understanding of the cellular networks underlying secretory organ form and function.

Authors:  Rajprasad Loganathan; Ji Hoon Kim; Michael B Wells; Deborah J Andrew
Journal:  Curr Top Dev Biol       Date:  2020-11-19       Impact factor: 4.897
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.