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Literature DB >> 99471

Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.

S Hayashi, I C Gillam, A D Delaney, G M Tener.

Abstract

DNA in prepared chromosomes from the larval salivary glands of Drosophila melanogaster was hybridized with [125I]-labeled 5S and tRNA from the same organism. Autoradiography revealed that radioactivity was frequently bound to all regions of the slides, masking labeling of the chromosomes. Acetylation of the preparations before hybridization prevented the formation of this background and revealed the specific chromosomal sites.

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Year: 1978 PMID： 99471 DOI： 10.1177/26.8.99471

Source DB: PubMed Journal: J Histochem Cytochem ISSN： 0022-1554 Impact factor: 2.479

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Cited

113 in total

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Authors: U Walldorf; B Hovemann; E K Bautz
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5. In situ hybridization methods for the detection of somatostatin mRNA in tissue sections using antisense RNA probes.

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6. The DNA-binding activity of transcription factor PTF1 parallels the synthesis of pancreas-specific mRNAs during mouse development.

Authors: S Petrucco; P K Wellauer; O Hagenbüchle
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Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.

1. Detection of enteroviruses in cell cultures by using in situ transcription.

2. DNA Replication-Dependent Histone H2A mRNA Expression in Pea Root Tips.

3. Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas.

4. F1 and F2: Two similar genes regulated differently during development of Drosophila melanogaster.

5. In situ hybridization methods for the detection of somatostatin mRNA in tissue sections using antisense RNA probes.

6. The DNA-binding activity of transcription factor PTF1 parallels the synthesis of pancreas-specific mRNAs during mouse development.

7. The LOX1 Gene of Arabidopsis Is Temporally and Spatially Regulated in Germinating Seedlings.

8. cAMP regulates P450scc gene expression by a cycloheximide-insensitive mechanism in cultured mouse Leydig MA-10 cells.

9. A DNA sequence of Drosophila melanogaster with a differential telomeric distribution.

10. Nucleolar localization of myc transcripts.