Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Radioimmunoassay and intramural distribution of PHI-IR in human intestine.

Literature DB >> 6688046

Radioimmunoassay and intramural distribution of PHI-IR in human intestine.

N D Christofides, Y Yiangou, E Aarons, G L Ferri, K Tatemoto, J M Polak, S R Bloom.

Abstract

The objective of this study was to develop a radioimmunoassay for PHI and use this to assess its intramural distribution in the human intestine. The antibody was harvested following immunization with porcine PHI conjugated to bovine serum albumin by glutaraldehyde, and the iodinated PHI tracer was prepared by the Iodo-gen method. The assay system showed no cross-reaction with other members of the glucagon-secretin family of peptides and was sensitive to changes of PHI of 2 fmol/tube (95% confidence). High concentrations of immunoreactive PHI were found in the human intestine, exclusively localized in the nonendocrine gut layers, suggesting a possible neuroendocrinological or neurotransmitter role for PHI.

Entities: Chemical Disease Species

Mesh：

Substances：
Peptide PHI
Peptides

Year: 1983 PMID： 6688046 DOI： 10.1007/bf01308152

Source DB: PubMed Journal: Dig Dis Sci ISSN： 0163-2116 Impact factor: 3.199

10 in total

1. Production and evaluation of antibodies for the radioimmunoassay of gastrin.

Authors: J F Rehfeld; F Stadil; B Rubin
Journal: Scand J Clin Lab Invest Date: 1972-10 Impact factor: 1.713

2. Tissue localization and relative distribution of regulatory peptides in separated layers from the human bowel.

Authors: G L Ferri; T E Adrian; M A Ghatei; D J O'Shaughnessy; L Probert; Y C Lee; A M Buchan; J M Polak; S R Bloom
Journal: Gastroenterology Date: 1983-04 Impact factor: 22.682

3. Actions of a newly isolated intestinal peptide PHI on pancreatic acini.

Authors: R T Jensen; K Tatemoto; V Mutt; G F Lemp; J D Gardner
Journal: Am J Physiol Date: 1981-12

4. Actions of a new peptide from porcine intestine (PHI) on pancreatic secretion in the rat and turkey.

Authors: R Dimaline; G J Dockray
Journal: Life Sci Date: 1980-11-24 Impact factor: 5.037

5. Cyclic AMP production in isolated colonic epithelial crypts: a highly sensitive model for the evaluation of vasoactive intestinal peptide action in human intestine.

Authors: C Dupont; M Laburthe; J P Broyart; D Bataille; G Rosselin
Journal: Eur J Clin Invest Date: 1980-02 Impact factor: 4.686

6. Porcine peptide having N-terminal histidine and C-terminal isoleucine amide (PHI): vasoactive intestinal peptide (VIP) and secretin-like effects in different tissues from the rat.

Authors: D Bataille; C Gespach; M Laburthe; B Amiranoff; K Tatemoto; N Vauclin; V Mutt; G Rosselin
Journal: FEBS Lett Date: 1980-06-02 Impact factor: 4.124

7. Interaction of a newly isolated intestinal polypeptide (PHI) with glucose and arginine to effect the secretion of insulin and glucagon.

Authors: J Szecówka; K Tatemoto; V Mutt; S Efendić
Journal: Life Sci Date: 1980-02-11 Impact factor: 5.037

8. Measurement of fasting and postprandial plasma VIP in man.

Authors: S J Mitchell; S R Bloom
Journal: Gut Date: 1978-11 Impact factor: 23.059

9. Isolation and characterization of the intestinal peptide porcine PHI (PHI-27), a new member of the glucagon--secretin family.

Authors: K Tatemoto; V Mutt
Journal: Proc Natl Acad Sci U S A Date: 1981-11 Impact factor: 11.205

10. Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril.

Authors: P J Fraker; J C Speck
Journal: Biochem Biophys Res Commun Date: 1978-02-28 Impact factor: 3.575

10 in total

10. Comparative distribution of vasoactive intestinal polypeptide (VIP), substance P and PHI in the enteric sphincters of the cat.

Authors: G P McGregor; A E Bishop; M A Blank; N D Christofides; Y Yiangou; J M Polak; S R Bloom
Journal: Experientia Date: 1984-05-15

10 in total

Radioimmunoassay and intramural distribution of PHI-IR in human intestine.

1. Production and evaluation of antibodies for the radioimmunoassay of gastrin.

2. Tissue localization and relative distribution of regulatory peptides in separated layers from the human bowel.

3. Actions of a newly isolated intestinal peptide PHI on pancreatic acini.

4. Actions of a new peptide from porcine intestine (PHI) on pancreatic secretion in the rat and turkey.

5. Cyclic AMP production in isolated colonic epithelial crypts: a highly sensitive model for the evaluation of vasoactive intestinal peptide action in human intestine.

6. Porcine peptide having N-terminal histidine and C-terminal isoleucine amide (PHI): vasoactive intestinal peptide (VIP) and secretin-like effects in different tissues from the rat.

7. Interaction of a newly isolated intestinal polypeptide (PHI) with glucose and arginine to effect the secretion of insulin and glucagon.

8. Measurement of fasting and postprandial plasma VIP in man.

9. Isolation and characterization of the intestinal peptide porcine PHI (PHI-27), a new member of the glucagon--secretin family.

10. Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril.

Review 1. Physiology and pathophysiology of colonic motor activity (1).

2. Decreased colonic peptide histidine-methionine in idiopathic inflammatory bowel diseases.

3. Peptide-immunoreactive nerves in the mammalian female genital tract.

4. Infusion of a novel peptide, PHI, in man. Pharmacokinetics and effect on gastric secretion.

5. Nerve fibres containing peptide histidine isoleucine (PHI) in the respiratory tract.

6. Effect of peptide histidine isoleucine on water and electrolyte transport in the human jejunum.

7. The presence of peptide histidine-isoleucine in human, dog, guinea-pig and rat pancreas.

8. Peptide histidine isoleucine (PHI). A secretagogue in porcine intestine.

9. Distribution of a novel pituitary protein (7B2) in mammalian gastrointestinal tract and pancreas.

10. Comparative distribution of vasoactive intestinal polypeptide (VIP), substance P and PHI in the enteric sphincters of the cat.