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Literature DB >> 6641717

Polynucleotide-histone H1 complexes as probes for blot hybridization.

Abstract

Biotin and/or 125I-labelled histone H1 proteins (21 kd) have been covalently bound to single-stranded DNA. Complexes of equal masses of DNA and modified histone H1 (approximately 1 histone H1 molecule per 70 nucleotides) were used as probes for blot hybridization experiments and found to have hybridization characteristics very similar (or identical) to radiolabelled DNA probes.

Entities: Chemical

Mesh：

Substances：

Year: 1983 PMID： 6641717 PMCID： PMC555195 DOI： 10.1002/j.1460-2075.1983.tb01508.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

14 in total

1. Studies on the role and mode of operation of the very-lysine-rich histone H1 (F1) in eukaryote chromatin. The properties of the N-terminal and C-terminal halves of histone H1.

Authors: E M Bradbury; G E Chapman; S E Danby; P G Hartman; P L Riches
Journal: Eur J Biochem Date: 1975-09-15

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. A method for gene enrichment based on the avidin-biotin interaction. Application to the Drosophila ribosomal RNA genes.

Authors: J Manning; M Pellegrini; N Davidson
Journal: Biochemistry Date: 1977-04-05 Impact factor: 3.162

4. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.

Authors: J C Alwine; D J Kemp; B A Parker; J Reiser; J Renart; G R Stark; G M Wahl
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

5. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.

Authors: F C Kafatos; C W Jones; A Efstratiadis
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

6. A new method of in situ hybridization.

Authors: J E Manning; N D Hershey; T R Broker; M Pellegrini; H K Mitchell; N Davidson
Journal: Chromosoma Date: 1975-11-24 Impact factor: 4.316

7. Irreversible inhibition of biotin transport in yeast by biotinyl-p-nitrophenyl ester.

Authors: J M Becker; M Wilchek; E Katchalski
Journal: Proc Natl Acad Sci U S A Date: 1971-10 Impact factor: 11.205

8. Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels.

Authors: T R Broker; L M Angerer; P H Yen; N D Hershey; N Davidson
Journal: Nucleic Acids Res Date: 1978-02 Impact factor: 16.971

9. Transition from noncooperative to cooperative and selective binding of histone H1 to DNA.

Authors: M Renz; L A Day
Journal: Biochemistry Date: 1976-07-27 Impact factor: 3.162

10. Alcohol dehydrogenase gene of Drosophila melanogaster: relationship of intervening sequences to functional domains in the protein.

Authors: C Benyajati; A R Place; D A Powers; W Sofer
Journal: Proc Natl Acad Sci U S A Date: 1981-05 Impact factor: 11.205

15 in total

1. Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin.

Authors: A C Forster; J L McInnes; D C Skingle; R H Symons
Journal: Nucleic Acids Res Date: 1985-02-11 Impact factor: 16.971

2. Protein-DNA conjugates produced by UV irradiation and their use as probes for hybridization.

Authors: J Czichos; M Köhler; B Reckmann; M Renz
Journal: Nucleic Acids Res Date: 1989-02-25 Impact factor: 16.971

3. Detection of sub-picogram quantities of specific DNA sequences on blot hybridization with biotinylated probes.

Authors: V T Chan; K A Fleming; J O McGee
Journal: Nucleic Acids Res Date: 1985-11-25 Impact factor: 16.971

4. Cooperative interaction of histone H1 with DNA.

Authors: F Watanabe
Journal: Nucleic Acids Res Date: 1986-04-25 Impact factor: 16.971

5. Fast quantification of nucleic acid hybrids by affinity-based hybrid collection.

Authors: A C Syvänen; M Laaksonen; H Söderlund
Journal: Nucleic Acids Res Date: 1986-06-25 Impact factor: 16.971

6. Time-resolved fluorometry: a sensitive method to quantify DNA-hybrids.

Authors: A C Syvänen; P Tchen; M Ranki; H Söderlund
Journal: Nucleic Acids Res Date: 1986-01-24 Impact factor: 16.971

7. Sensitive non-radioactive dot-blot hybridization using DNA probes labelled with chelate group substituted psoralen and quantitative detection by europium ion fluorescence.

Authors: A Oser; W K Roth; G Valet
Journal: Nucleic Acids Res Date: 1988-02-11 Impact factor: 16.971