Literature DB >> 2933635

Detection of sub-picogram quantities of specific DNA sequences on blot hybridization with biotinylated probes.

V T Chan, K A Fleming, J O McGee.   

Abstract

A sensitive method for detecting biotinylated DNA probes on dot and Southern blots is described which is based on the principle outlined by Leary et al (1). This system has two main components: detection of biotinylated DNA by a two-step procedure with streptavidin and poly(alkaline phosphatase); and blocking background with Tween 20. 32fg and 80fg of lambda phage DNA was detected on dot and Southern blot hybridizations respectively. 150fg of beta-globin was detected on Southern blots of genomic DNA. This method is fast, reproducible and can detect single copy genes in 0.25 micrograms genomic DNA on Southern blots.

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Year:  1985        PMID： 2933635      PMCID： PMC322111          DOI： 10.1093/nar/13.22.8083

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  28 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Application of the avidin-biotin method of gene enrichment to the isolation of long double-stranded DNA containing specific gene sequences.

Authors:  M Pellegrini; D S Holmes; J Manning
Journal:  Nucleic Acids Res       Date:  1977-09       Impact factor: 16.971

3.  Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors:  G M Wahl; M Stern; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

4.  Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells.

Authors:  J Burns; V T Chan; J A Jonasson; K A Fleming; S Taylor; J O McGee
Journal:  J Clin Pathol       Date:  1985-10       Impact factor: 3.411

5.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

6.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

Authors:  S M Hsu; L Raine; H Fanger
Journal:  J Histochem Cytochem       Date:  1981-04       Impact factor: 2.479

7.  Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genes.

Authors:  R E Kaufman; P J Kretschmer; J W Adams; H C Coon; W F Anderson; A W Nienhuis
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

8.  The use of the avidin-biotin complex as a tool in molecular biology.

Authors:  E A Bayer; M Wilchek
Journal:  Methods Biochem Anal       Date:  1980

9.  A new method of in situ hybridization.

Authors:  J E Manning; N D Hershey; T R Broker; M Pellegrini; H K Mitchell; N Davidson
Journal:  Chromosoma       Date:  1975-11-24       Impact factor: 4.316

10.  Nucleic acid guanine: reaction with the carcinogen N-acetoxy-2-acetylaminofluorene.

Authors:  E C Miller; U Juhl; J A Miller
Journal:  Science       Date:  1966-09-02       Impact factor: 47.728
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  35 in total

Review 1.  Molecular techniques in the diagnosis of human infectious diseases.

Authors:  U Desselberger; K Collingham
Journal:  Genitourin Med       Date:  1990-10

2.  Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridisation.

Authors:  J Burns; A K Graham; C Frank; K A Fleming; M F Evans; J O McGee
Journal:  J Clin Pathol       Date:  1987-08       Impact factor: 3.411

3.  In situ human papillomavirus (HPV) genotyping of cervical intraepithelial neoplasia in South African and British patients: evidence for putative HPV integration in vivo.

Authors:  K Cooper; C S Herrington; A K Graham; M F Evans; J O McGee
Journal:  J Clin Pathol       Date:  1991-05       Impact factor: 3.411

4.  [Technic of nucleic acid hybridization and its significance for diagnostic problems].

Authors:  R Neumann
Journal:  Naturwissenschaften       Date:  1987-03

5.  Extrachromosomal plasmid-like DNA in the obligate parasitic fungus Erysiphe graminis f. sp. hordei.

Authors:  H Giese; S K Christiansen; H P Jensen
Journal:  Theor Appl Genet       Date:  1990-01       Impact factor: 5.699

6.  Model system for optimising mRNA non-isotopic in situ hybridisation: riboprobe detection of lysozyme mRNA in archival gut biopsy specimens.

Authors:  J C Martinez-Montero; C S Herrington; J Stickland; H Sawyer; M Evans; D M Flannery; J O McGee
Journal:  J Clin Pathol       Date:  1991-10       Impact factor: 3.411

7.  Discrimination of closely homologous HPV types by nonisotopic in situ hybridization: definition and derivation of tissue melting temperatures.

Authors:  C S Herrington; A K Graham; D M Flannery; J Burns; J O McGee
Journal:  Histochem J       Date:  1990-10

8.  Glomerular expression of interleukin-1 receptor antagonist and interleukin-1 beta genes in antibody-mediated glomerulonephritis.

Authors:  F W Tam; J Smith; S J Cashman; Y Wang; E M Thompson; A J Rees
Journal:  Am J Pathol       Date:  1994-07       Impact factor: 4.307

9.  Interphase cytogenetics using biotin and digoxigenin labelled probes: III. Increased sensitivity and flexibility for detecting HPV in cervical biopsy specimens and cell lines.

Authors:  C S Herrington; A K Graham; J O McGee
Journal:  J Clin Pathol       Date:  1991-01       Impact factor: 3.411

10.  The application of alkaline phosphatase labeled HBV probe in serum detection.

Authors:  Zhiming Tu; Li-Hua Ke; Guangyuan He
Journal:  Virus Genes       Date:  2004-03       Impact factor: 2.332
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