Literature DB >> 3945551

Time-resolved fluorometry: a sensitive method to quantify DNA-hybrids.

A C Syvänen, P Tchen, M Ranki, H Söderlund.   

Abstract

Europium and other lanthanides can be excitated with UV-radiation, whereafter the energy is released as fluorescence, delayed in time up to 1 ms after the excitation. Eu can be used as a sensitive label in biological assays. Here we report on the application of time-resolved fluorometry to detect nucleic acid hybrids. The probe DNA was tagged with a hapten, either a fluorene or a sulfone group. After hybridization the probe DNA was detected by a two-step immunological assay with the second antibody labelled with Eu. The method is quantitative with a detection limit of 0.3 pg of actual target regions of immobilized adenovirus genomic DNA. The label was also used in sandwich hybridization, which allowed analyzing nasopharyngeal mucus for the presence of adenovirus.

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Year:  1986        PMID： 3945551      PMCID： PMC339480          DOI： 10.1093/nar/14.2.1017

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins.

Authors:  H Hjelm; K Hjelm; J Sjöquist
Journal:  FEBS Lett       Date:  1972-11-15       Impact factor: 4.124

2.  Novel test for rapid viral diagnosis: detection of adenovirus in nasopharyngeal mucus aspirates by means of nucleic-acid sandwich hybridisation.

Authors:  M Virtanen; A Palva; M Laaksonen; P Halonen; H Söderlund; M Ranki
Journal:  Lancet       Date:  1983-02-19       Impact factor: 79.321

3.  Time-resolved fluorometer for lanthanide chelates--a new generation of nonisotopic immunoassays.

Authors:  E Soini; H Kojola
Journal:  Clin Chem       Date:  1983-01       Impact factor: 8.327

Review 4.  Fluoroimmunoassay: present status and key problems.

Authors:  E Soini; I Hemmilä
Journal:  Clin Chem       Date:  1979-03       Impact factor: 8.327

5.  The making of strand-specific M13 probes.

Authors:  N Hu; J Messing
Journal:  Gene       Date:  1982-03       Impact factor: 3.688

6.  Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.

Authors:  F C Kafatos; C W Jones; A Efstratiadis
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

7.  Reactivity of the antibodies to DNA modified by the carcinogen N-acetoxy-N-acetyl-2-aminofluorene.

Authors:  E Sage; R P Fuchs; M Leng
Journal:  Biochemistry       Date:  1979-04-03       Impact factor: 3.162

8.  Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Authors:  P R Langer; A A Waldrop; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

9.  Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.

Authors:  D J Brigati; D Myerson; J J Leary; B Spalholz; S Z Travis; C K Fong; G D Hsiung; D C Ward
Journal:  Virology       Date:  1983-04-15       Impact factor: 3.616

10.  Sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples.

Authors:  M Ranki; A Palva; M Virtanen; M Laaksonen; H Söderlund
Journal:  Gene       Date:  1983 Jan-Feb       Impact factor: 3.688
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  9 in total

1.  Preparation of europium-labelled DNA probes and their properties.

Authors:  P Hurskainen; P Dahlén; J Ylikoski; M Kwiatkowski; H Siitari; T Lövgren
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

Review 2.  Diagnostic deoxyribonucleic acid probes for infectious diseases.

Authors:  F C Tenover
Journal:  Clin Microbiol Rev       Date:  1988-01       Impact factor: 26.132

3.  Time-resolved fluorometry for the identification of viral DNA in clinical specimens.

Authors:  P Dahlén; P Hurskainen; T Lövgren; T Hyypiä
Journal:  J Clin Microbiol       Date:  1988-11       Impact factor: 5.948

4.  Fast quantification of nucleic acid hybrids by affinity-based hybrid collection.

Authors:  A C Syvänen; M Laaksonen; H Söderlund
Journal:  Nucleic Acids Res       Date:  1986-06-25       Impact factor: 16.971

5.  Sensitive non-radioactive dot-blot hybridization using DNA probes labelled with chelate group substituted psoralen and quantitative detection by europium ion fluorescence.

Authors:  A Oser; W K Roth; G Valet
Journal:  Nucleic Acids Res       Date:  1988-02-11       Impact factor: 16.971

6.  Quantification of nucleic acids on nitrocellulose membranes with time-resolved fluorometry.

Authors:  T K Christopoulos; E P Diamandis; G Wilson
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971

7.  Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.

Authors:  E Jablonski; E W Moomaw; R H Tullis; J L Ruth
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

8.  1,N6-etheno deoxy and ribo adenosine and 3,N4-etheno deoxy and ribo cytidine phosphoramidites. Strongly fluorescent structures for selective introduction in defined sequence DNA and RNA molecules.

Authors:  S C Srivastava; S K Raza; R Misra
Journal:  Nucleic Acids Res       Date:  1994-04-11       Impact factor: 16.971

9.  Tentacle probe sandwich assay in porous polymer monolith improves specificity, sensitivity and kinetics.

Authors:  Brent C Satterfield; Michael R Caplan; Jay A A West
Journal:  Nucleic Acids Res       Date:  2008-09-12       Impact factor: 16.971
  9 in total

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