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Literature DB >> 2922287

Protein-DNA conjugates produced by UV irradiation and their use as probes for hybridization.

J Czichos¹, M Köhler, B Reckmann, M Renz.

Abstract

Several proteins have been crosslinked to DNA by low dose uv irradiation. The principle of the method is based on an efficient and fast radiation induced reaction of amino acid residues with DNA at low pH. The method seems to be of general applicability for crosslinking proteins to DNA in a very simple one step procedure. Some of such DNA-protein conjugates have been used as probes for hybridization experiments. DNA-protein A probes were found to be most useful.

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Year: 1989 PMID： 2922287 PMCID： PMC331822 DOI： 10.1093/nar/17.4.1563

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

20 in total

1. Photoinduced convalent crosslinkage, in situ, of Escherichia coli 50 S ribosomal proteins to rRNA.

Authors: L Gorelic
Journal: Biochim Biophys Acta Date: 1975-05-01

2. Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin.

Authors: A C Forster; J L McInnes; D C Skingle; R H Symons
Journal: Nucleic Acids Res Date: 1985-02-11 Impact factor: 16.971

3. Ultraviolet light-induced stable complexes of DNA and DNA polymerase.

Authors: A Markovitz
Journal: Biochim Biophys Acta Date: 1972-11-09

4. Induction of stable linkage between the deoxyribonucleic acid dependent ribonucleic acid polymerase and d(A-T)n-d(A-T)n by ultraviolet light.

Authors: G F Strniste; D A Smith
Journal: Biochemistry Date: 1974-01-29 Impact factor: 3.162

5. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

Authors: J A Spudich; S Watt
Journal: J Biol Chem Date: 1971-08-10 Impact factor: 5.157

10. Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

Authors: J J Leary; D J Brigati; D C Ward
Journal: Proc Natl Acad Sci U S A Date: 1983-07 Impact factor: 11.205

1 in total

1. Crosslinking of DNA and proteins induced by protein hydroperoxides.

Authors: S Gebicki; J M Gebicki
Journal: Biochem J Date: 1999-03-15 Impact factor: 3.857

1 in total

Protein-DNA conjugates produced by UV irradiation and their use as probes for hybridization.

1. Photoinduced convalent crosslinkage, in situ, of Escherichia coli 50 S ribosomal proteins to rRNA.

2. Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin.

3. Ultraviolet light-induced stable complexes of DNA and DNA polymerase.

4. Induction of stable linkage between the deoxyribonucleic acid dependent ribonucleic acid polymerase and d(A-T)n-d(A-T)n by ultraviolet light.

5. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

Review 6. Analytical strategies for the use of DNA probes.

7. Chemically modified nucleic acids as immunodetectable probes in hybridization experiments.

8. A colorimetric method for DNA hybridization.

9. Photochemical attachment of lac repressor to bromodeoxyuridine-substituted lac operator by ultraviolet radiation.

10. Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

1. Crosslinking of DNA and proteins induced by protein hydroperoxides.