Literature DB >> 6374657

Chemically modified nucleic acids as immunodetectable probes in hybridization experiments.

P Tchen, R P Fuchs, E Sage, M Leng.   

Abstract

Guanine residues in nucleic acids can be modified by treatment with N-acetoxy-N-2-acetylaminofluorene and its 7-iodo derivative in an in vitro nonenzymatic reaction. The modified nucleic acids (ribo or deoxyribo, single or double stranded) are recognized by specific antibodies. They can be immunoprecipitated or used as probes in hybridization experiments and detected by immunochemical techniques.

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Year:  1984        PMID： 6374657      PMCID： PMC345529          DOI： 10.1073/pnas.81.11.3466

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  Comparative studies on the 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene: binding sites on DNA and conformational change of modified deoxytrinucleotides.

Authors:  J F Lefèvre; R P Fuchs; M P Daune
Journal:  Biochemistry       Date:  1978-06-27       Impact factor: 3.162

Review 2.  Carcinogenesis by aromatic amines.

Authors:  E Kriek
Journal:  Biochim Biophys Acta       Date:  1974-09-09

3.  Physical studies on deoxyribonucleic acid after covalent binding of a carcinogen.

Authors:  R Fuchs; M Daune
Journal:  Biochemistry       Date:  1972-07-04       Impact factor: 3.162

4.  Physical basis of chemical carcinogenesis by N-2-fluorenylacetamide derivatives and analogs.

Authors:  R Fuchs; M Daune
Journal:  FEBS Lett       Date:  1973-08-15       Impact factor: 4.124

5.  Effect on N-7 and C-8 substitution of guanine in DNA on Tm, buoyant density and RNA polymerase priming.

Authors:  W Troll; E Rinde; P Day
Journal:  Biochim Biophys Acta       Date:  1969-01-21

6.  The reaction of the carcinogen N-acetoxy-2-acetyl-aminofluorene with DNA and other polynucleotides and its stereochemical implications.

Authors:  A M Kapuler; A M Michelson
Journal:  Biochim Biophys Acta       Date:  1971-03-25

7.  Nucleic acid guanine: reaction with the carcinogen N-acetoxy-2-acetylaminofluorene.

Authors:  E C Miller; U Juhl; J A Miller
Journal:  Science       Date:  1966-09-02       Impact factor: 47.728

8.  8-(N-2-fluorenylacetamido)guanosine, an arylamidation reaction product of guanosine and the carcinogen N-acetoxy-N-2-fluorenylacetamide in neutral solution.

Authors:  E Kriek; J A Miller; U Juhl; E C Miller
Journal:  Biochemistry       Date:  1967-01       Impact factor: 3.162

9.  Comparative orientation of the fluorene residue in native DNA modified by N-acetoxy-N-2-acetylaminofluorene and two 7-halogeno derivatives.

Authors:  R P Fuchs; J F Lefevre; J Pouyet; M P Daune
Journal:  Biochemistry       Date:  1976-07-27       Impact factor: 3.162

10.  Polynucleotide-histone H1 complexes as probes for blot hybridization.

Authors:  M Renz
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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  41 in total

1.  Usefulness of the ID32 staph system and a method based on rRNA gene restriction site polymorphism analysis for species and subspecies identification of staphylococcal clinical isolates.

Authors:  O Chesneau; S Aubert; A Morvan; J L Guesdon; N el Solh
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

2.  Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with biotinylated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy.

Authors:  E Viegas-Pequignot; B Dutrillaux; H Magdelenat; M Coppey-Moisan
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

3.  P-element distribution in Eurasian populations of Drosophila melanogaster: A genetic and molecular analysis.

Authors:  D Anxolabéhère; D Nouaud; G Périquet; P Tchen
Journal:  Proc Natl Acad Sci U S A       Date:  1985-08       Impact factor: 11.205

4.  Detection of mRNA and hnRNA using a digoxigenin labelled cDNA probe by in situ hybridization on frozen tissue sections.

Authors:  N Maggiano; L M Larocca; M Piantelli; F O Ranelletti; L Lauriola; R Ricci; A Capelli
Journal:  Histochem J       Date:  1991-02

5.  Preparation of europium-labelled DNA probes and their properties.

Authors:  P Hurskainen; P Dahlén; J Ylikoski; M Kwiatkowski; H Siitari; T Lövgren
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

6.  [Technic of nucleic acid hybridization and its significance for diagnostic problems].

Authors:  R Neumann
Journal:  Naturwissenschaften       Date:  1987-03

7.  Nucleotide sequence of an heterochromatic segment recognized by the antibodies to Z-DNA in fixed metaphase chromosomes.

Authors:  B Malfoy; N Rousseau; N Vogt; E Viegas-Pequignot; B Dutrillaux; M Leng
Journal:  Nucleic Acids Res       Date:  1986-04-25       Impact factor: 16.971

8.  Probability of recovering pathogenic Escherichia coli from foods.

Authors:  W E Hill; J L Ferreira; W L Payne; V M Jones
Journal:  Appl Environ Microbiol       Date:  1985-06       Impact factor: 4.792

9.  Non-radioactive in situ hybridization. A comparison of several immunocytochemical detection systems using reflection-contrast and electron microscopy.

Authors:  A F Cremers; N Jansen in de Wal; J Wiegant; R W Dirks; P Weisbeek; M van der Ploeg; J E Landegent
Journal:  Histochemistry       Date:  1987

10.  The application of alkaline phosphatase labeled HBV probe in serum detection.

Authors:  Zhiming Tu; Li-Hua Ke; Guangyuan He
Journal:  Virus Genes       Date:  2004-03       Impact factor: 2.332
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