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Literature DB >> 6633171

Detection and determination of lipase (acylglycerol hydrolase) activity from various sources.

Abstract

Methods for the detection and determination of lipases (acylglycerol hydrolases) and preparation of assays are reviewed including substrates, conditions and screening. Some newer methods for the determination of lipase activity are discussed. Several of these are: (a) titrimetry, (b) colorimetry of Cu soaps of free fatty acids (FFA), (c) colorimetry of chromophores in the acyl chain of FFA or in glycerol, (d) radioassay, (e) gas liquid chromatography, (f) enzymatic treatment of FFA and measurement of the resulting products, and (g) direct immunological determination of the lipase. Examples and sensitivities are given and advantages and disadvantages are described.

Entities: Chemical

Mesh：

Substances：
Fatty Acids
Copper
Lipase

Year: 1983 PMID： 6633171 DOI： 10.1007/BF02534677

Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880

50 in total

1. Purification of triglycerides with an alumina column.

Authors: R G Jensen; T A Marks; J Sampugna; J G Quinn; D L Carpenter
Journal: Lipids Date: 1966-11 Impact factor: 1.880

2. Hydrolysis of animal fat and vegetable oil with Mucor miehei esterase. Properties of the enzyme.

Authors: G J Moskowitz; R Cassaigne; I R West; T Shen; L I Feldman
Journal: J Agric Food Chem Date: 1977 Sep-Oct Impact factor: 5.279

3. Effect of albumin on products formed from chylomicron triacylclycerol by lipoprotein lipase in vitro.

Authors: R O Scow; T Olivecrona
Journal: Biochim Biophys Acta Date: 1977-06-22

4. Quantitative determination of serum triglycerides by the use of enzymes.

Authors: G Bucolo; H David
Journal: Clin Chem Date: 1973-05 Impact factor: 8.327

5. Methods for isolation and enumeration of lipolytic organisms.

Authors: T F Fryer; R C Lawrence; B Reiter
Journal: J Dairy Sci Date: 1967-04 Impact factor: 4.034

6. Fluorimetric assay of the activity of extracellular lipases of Pseudomonas fluorescens and Serratia marcescens.

Authors: R N Roy
Journal: J Appl Bacteriol Date: 1980-10

7. A micromethod using gas-liquid chromatography for measuring individual fatty acids liberated during interaction of triglyceride-rich lipoproteins and lipoprotein lipase.

Authors: M L Kashyap; M J Mellies; D Brady; B A Hynd; K Robinson
Journal: Anal Biochem Date: 1980-09-15 Impact factor: 3.365

8. Evaluation of five enzymic kits for determination of triglyceride concentrations in plasma.

Authors: R E Walker; P S Bachorik; P O Kwiterovich
Journal: Clin Chem Date: 1982-11 Impact factor: 8.327

9. Effects of divalent cations and sodium taurocholate on pancreatic lipase activity with gum arabic-emulsified tributyrylglycerol substrates.

Authors: W J Brown; A A Belmonte; P Melius
Journal: Biochim Biophys Acta Date: 1977-02-23

10. Assay of enzymes of lipid metabolism with colored and fluorescent derivatives of natural lipids.

Authors: S Gatt; Y Barenholz; R Goldberg; T Dinur; G Besley; Z Leibovitz-Ben Gershon; J Rosenthal; R J Desnick; E A Devine; B Shafit-Zagardo; F Tsuruki
Journal: Methods Enzymol Date: 1981 Impact factor: 1.600

17 in total

1. Microwave incubation improves lipolytic enzyme preservation for ultrastructural cytochemistry.

Authors: U A Rassner; D A Crumrine; P Nau; P M Elias
Journal: Histochem J Date: 1997-05

2. Specific and sensitive plate assay for bacterial lipases.

Authors: G Kouker; K E Jaeger
Journal: Appl Environ Microbiol Date: 1987-01 Impact factor: 4.792

3. An improved method for the colorimetric assay of lipase activity using an optically clear medium.

Authors: G Renard; J Grimaud; A el Zant; M Pina; J Graille
Journal: Lipids Date: 1987-07 Impact factor: 1.880

4. Purification and characterisation of an F16L mutant of a thermostable lipase.

Authors: Mohd Shukuri Mohamad Ali; Chong Chai Yun; Adam Leow Thean Chor; Raja Noor Zaliha Raja Abdul Rahman; Mahiran Basri; Abu Bakar Salleh
Journal: Protein J Date: 2012-03 Impact factor: 2.371

5. A clp gene homologue belonging to the Crp gene family globally regulates lytic enzyme production, antimicrobial activity, and biological control activity expressed by Lysobacter enzymogenes strain C3.

Authors: Donald Y Kobayashi; Ralph M Reedy; Jeffrey D Palumbo; Jun-Ma Zhou; Gary Y Yuen
Journal: Appl Environ Microbiol Date: 2005-01 Impact factor: 4.792

10. Characterization of lipases from the lipid bodies and microsomal membranes of erucic acid-free oilseed-rape (Brassica napus) cotyledons.

Authors: M J Hills; D J Murphy
Journal: Biochem J Date: 1988-02-01 Impact factor: 3.857