Literature DB >> 3103532

Specific and sensitive plate assay for bacterial lipases.

G Kouker, K E Jaeger.   

Abstract

A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby allowing quantitation of lipase activities ranging from 1 to 30 nkat.

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Year:  1987        PMID： 3103532      PMCID： PMC203632          DOI： 10.1128/aem.53.1.211-213.1987

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates.

Authors:  G SIERRA
Journal:  Antonie Van Leeuwenhoek       Date:  1957       Impact factor: 2.271

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Authors:  T F Kuimova; E A Shabanova; G A Kazakov
Journal:  Mikrobiologiia       Date:  1975 Mar-Apr

3.  The Action of Certain Bacteria on Some Simple Tri-glycerides and Natural Fats, as Shown by Nile-blue Sulphate.

Authors:  M A Collins; B W Hammer
Journal:  J Bacteriol       Date:  1934-05       Impact factor: 3.490

4.  A double layered plate method for the detection of microbial lipolysis.

Authors:  J A Alford; E E Steinle
Journal:  J Appl Bacteriol       Date:  1967-12

5.  Detection and determination of lipase (acylglycerol hydrolase) activity from various sources.

Authors:  R G Jensen
Journal:  Lipids       Date:  1983-09       Impact factor: 1.880

6.  Ultrathin-layer agar gels: a novel print technique for ultrathin-layer isoelectric focusing of enzymes.

Authors:  M Höfelmann; R Kittsteiner-Eberle; P Schreier
Journal:  Anal Biochem       Date:  1983-01       Impact factor: 3.365

7.  Estimation of lipase activity by the diffusion plate method.

Authors:  J Karnetová; J Matĕjů; T Rezanka; P Procházka; M Nohýnek; J Rokos
Journal:  Folia Microbiol (Praha)       Date:  1984       Impact factor: 2.099

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Authors:  H Wand; A Gabert; H Uhlig
Journal:  Z Allg Mikrobiol       Date:  1980

9.  A new assay of microbial lipases with emulsified trioleoyl glycerol.

Authors:  N Peled; M C Krenz
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

10.  Rapid colorimetric micromethod for free fatty acids.

Authors:  R D Mackenzie; T R Blohm; E M Auxier; A C Luther
Journal:  J Lipid Res       Date:  1967-11       Impact factor: 5.922
  10 in total
  116 in total

1.  Purification and characterization of an extracellular lipase from Penicillium candidum.

Authors:  B Ruiz; A Farrés; E Langley; F Masso; S Sánchez
Journal:  Lipids       Date:  2001-03       Impact factor: 1.880

2.  Phylogenetic and physiological diversity of microorganisms isolated from a deep greenland glacier ice core.

Authors:  V I Miteva; P P Sheridan; J E Brenchley
Journal:  Appl Environ Microbiol       Date:  2004-01       Impact factor: 4.792

3.  The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus.

Authors:  J Ron Bradner; Philip J L Bell; V S Junior Te'o; K M Helena Nevalainen
Journal:  Curr Genet       Date:  2003-09-17       Impact factor: 3.886

4.  Diversity and cold-active hydrolytic enzymes of culturable bacteria associated with Arctic sea ice, Spitzbergen.

Authors:  Tatiana Groudieva; Margarita Kambourova; Hoda Yusef; Maryna Royter; Ralf Grote; Hauke Trinks; Garabed Antranikian
Journal:  Extremophiles       Date:  2004-07-14       Impact factor: 2.395

5.  Deciphering the metabolic capabilities of a lipase producing Pseudomonas aeruginosa SL-72 strain.

Authors:  Shikha Verma; Radha Prasanna; Jyoti Saxena; Vinay Sharma; Lata Nain
Journal:  Folia Microbiol (Praha)       Date:  2012-06-02       Impact factor: 2.099

6.  Secreted fungal effector lipase releases free fatty acids to inhibit innate immunity-related callose formation during wheat head infection.

Authors:  Antje Blümke; Christian Falter; Cornelia Herrfurth; Björn Sode; Rainer Bode; Wilhelm Schäfer; Ivo Feussner; Christian A Voigt
Journal:  Plant Physiol       Date:  2014-03-31       Impact factor: 8.340

7.  Cloning and expression of a novel lipase gene from Pseudomonas fluorescens B52.

Authors:  Zhengbing Jiang; Yitao Zheng; Yu Luo; Gang Wang; Hongping Wang; Yushu Ma; Dongzhi Wei
Journal:  Mol Biotechnol       Date:  2005-10       Impact factor: 2.695

Review 8.  New extremophilic lipases and esterases from metagenomics.

Authors:  Olalla López-López; Maria E Cerdán; Maria I González Siso
Journal:  Curr Protein Pept Sci       Date:  2014       Impact factor: 3.272

9.  Purification and specificity of lipases fromGeotrichum candidum.

Authors:  M W Baillargeon
Journal:  Lipids       Date:  1990-12       Impact factor: 1.880

10.  Gene cloning, sequence analysis, purification, and secretion by Escherichia coli of an extracellular lipase from Serratia marcescens.

Authors:  X Li; S Tetling; U K Winkler; K E Jaeger; M J Benedik
Journal:  Appl Environ Microbiol       Date:  1995-07       Impact factor: 4.792
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