| Literature DB >> 3626781 |
G Renard, J Grimaud, A el Zant, M Pina, J Graille.
Abstract
Lipase activity can be spectrophometrically measured in an optically clear medium using long chain fatty thioesters of 1-mercapto-2,3-propanediol or 2-mercaptoethanol as substrates. With hexamethylphosphoric triamide solutions of these thiosubstrates, the Michaëlis-Menten constants of lipase from Rhizopus arrhizus were determined. The effects of calcium chloride and of bovine serum albumin on the enzyme activity were established.Entities:
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Year: 1987 PMID： 3626781 DOI： 10.1007/BF02540372
Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880