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Literature DB >> 640376

Alcohol dehydrogenase mutants of Chinese hamster somatic cells resistant to allyl alcohol.

Abstract

Alcohol dehydrogenase (alcohol: NAD oxidoreductase, E.C. 1.1.1.1.) mutants of Chinese hamster somatic cells were isolated as resistant to allyl alcohol (ALLR). The ALLR phenotypes of the mutant clones were reproducible with high fidelity and stable over long intervals of growth in the absence of the selecting drug. Several mutants, Adh-1, Adh-2, Adh-9 and Adh-13, resistant to allyl alcohol were characterized. They have between 15 and 40% of the alcohol dehydrogenase activity of the wild-type cell lines. This phenotype is therefore a useful marker to analyze gene segregation of somatic cell mutations and to study the expression of the genes involved in the metabolism of ethanol in mammalian cells.

Entities: Chemical Gene Species

Mesh：

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Year: 1978 PMID： 640376 PMCID： PMC1213805

Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562

13 in total

1. Chemical selection of mutants that affect alcohol dehydrogenase in Drosophila. II. Use of 1-pentyne-3-ol.

Authors: J O'Donnell; L Gerace; F Leister; W Sofer
Journal: Genetics Date: 1975-01 Impact factor: 4.562

2. Stable alterations at the cell membrane of Chinese hamster ovary cells resistant to the cytotoxicity of phytohemagglutinin.

Authors: P Stanley; V Caillibot; L Siminovitch
Journal: Somatic Cell Genet Date: 1975-01

Review 3. On the nature of hereditable variation in cultured somatic cells.

Authors: L Siminovitch
Journal: Cell Date: 1976-01 Impact factor: 41.582

4. Differential staining of human and mouse chromosomes in interspecific cell hybrids.

Authors: M Bobrow; J Cross
Journal: Nature Date: 1974-09-06 Impact factor: 49.962

5. Mutants partially deficient in alcohol dehydrogenase in Schizosaccharomyces pombe.

Authors: R Megnet
Journal: Arch Biochem Biophys Date: 1967-07 Impact factor: 4.013

6. [Heterogeneity and organ distribution of alcohol dehydrogenase in various species].

Authors: K Moser; J Papenberg; J P von Wartburg
Journal: Enzymol Biol Clin (Basel) Date: 1968

7. Parasexual cycle in cultivated human somatic cells.

Authors: G M Martin; C A Sprague
Journal: Science Date: 1969-11-07 Impact factor: 47.728

8. Regulation of expression of Adh genes in maize.

Authors: D Schwartz
Journal: Proc Natl Acad Sci U S A Date: 1976-02 Impact factor: 11.205

9. Purine mutants of mammalian cell lines: III. Control of purine biosynthesis in adenine phosphoribosyl transferase mutants of CHO cells.

Authors: M W Taylor; J H Pipkorn; M K Tokito; R O Pozzatti
Journal: Somatic Cell Genet Date: 1977-03

Alcohol dehydrogenase mutants of Chinese hamster somatic cells resistant to allyl alcohol.

1. Chemical selection of mutants that affect alcohol dehydrogenase in Drosophila. II. Use of 1-pentyne-3-ol.

2. Stable alterations at the cell membrane of Chinese hamster ovary cells resistant to the cytotoxicity of phytohemagglutinin.

Review 3. On the nature of hereditable variation in cultured somatic cells.

4. Differential staining of human and mouse chromosomes in interspecific cell hybrids.

5. Mutants partially deficient in alcohol dehydrogenase in Schizosaccharomyces pombe.

6. [Heterogeneity and organ distribution of alcohol dehydrogenase in various species].

7. Parasexual cycle in cultivated human somatic cells.

8. Regulation of expression of Adh genes in maize.

9. Purine mutants of mammalian cell lines: III. Control of purine biosynthesis in adenine phosphoribosyl transferase mutants of CHO cells.

10. Chemical selection of alcohol dehydrogenase negative mutants in drosophila.

1. Cross-reactive monoclonal antibodies to alcohol dehydrogenases.

2. Purification and properties of two distinct groups of ADH isozymes from Chinese hamster liver.

3. Comparison of the properties of the alcohol dehydrogenases from wild-type and mutant Chinese hamster somatic cells.

4. Allyl Alcohol Selection for Lower Alcohol Dehydrogenase Activity in Nicotiana plumbaginifolia Cultured Cells.

5. Adenovirus-induced mutations at the hypoxanthine phosphoribosyltransferase locus of Chinese hamster cells.

6. Characterization of alcohol dehydrogenase in young soybean seedlings.