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Literature DB >> 44189

Comparison of the properties of the alcohol dehydrogenases from wild-type and mutant Chinese hamster somatic cells.

Abstract

Alcohol dehydrogenases (alcohol: NAD oxidoreductase, E.C. 1.1.1.1.) from allyl alcohol-resistant and wild-type Chinese hamster cells were purified using gel filtration, ion-exchange, and affinity-column chromatography. Both enzymes exhibited the same isozyme band patterns on electrophoresis and isoelectric focusing. Physicochemical properties of the two enzymes such as pH and temperature optima, Km values, and temperature stability were found to be the same within the experimental errors. The genetic significance of these findings is discussed.

Entities: Chemical Species

Mesh：

Substances：
Alcohol Oxidoreductases

Year: 1979 PMID： 44189 DOI： 10.1007/bf00504305

Source DB: PubMed Journal: Biochem Genet ISSN： 0006-2928 Impact factor: 1.890

23 in total

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors: B J DAVIS
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

2. Enzymology of human alcohol metabolism.

Authors: T K li
Journal: Adv Enzymol Relat Areas Mol Biol Date: 1977

3. Microheterogeneity and allomorphism of proteins.

Authors: A R Williamson; M R Salaman; H W Kreth
Journal: Ann N Y Acad Sci Date: 1973-06-15 Impact factor: 5.691

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. Human and mouse hypoxanthine-guanine phosphoribosyltransferase: dimers and tetramers.

Authors: G G Johnson; L R Eisenberg; B R Migeon
Journal: Science Date: 1979-01-12 Impact factor: 47.728

6. Genetic and cytogenetic analysis of the Adh region in Drosophila melanogaster.

Authors: J O'Donnell; H C Mandel; M Krauss; W Sofer
Journal: Genetics Date: 1977-07 Impact factor: 4.562

7. Alcohol dehydrogenase in maize: genetic control of enzyme activity.

Authors: Y Efron
Journal: Science Date: 1970-11-13 Impact factor: 47.728

8. Cis-dominant regulatory mutations affecting the formation of glucose-repressible alcohol dehydrogenase (ADHII) in Saccharomyces cerevisiae.

Authors: M Ciriacy
Journal: Mol Gen Genet Date: 1976-06-15

Comparison of the properties of the alcohol dehydrogenases from wild-type and mutant Chinese hamster somatic cells.

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

2. Enzymology of human alcohol metabolism.

3. Microheterogeneity and allomorphism of proteins.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Human and mouse hypoxanthine-guanine phosphoribosyltransferase: dimers and tetramers.

6. Genetic and cytogenetic analysis of the Adh region in Drosophila melanogaster.

7. Alcohol dehydrogenase in maize: genetic control of enzyme activity.

8. Cis-dominant regulatory mutations affecting the formation of glucose-repressible alcohol dehydrogenase (ADHII) in Saccharomyces cerevisiae.

9. Regulation of expression of Adh genes in maize.

10. One cell-one immunoglobulin. Origin of limited heterogeneity of myeloma proteins.

1. Purification and properties of two distinct groups of ADH isozymes from Chinese hamster liver.

2. Characterization of alcohol dehydrogenase in young soybean seedlings.