Literature DB >> 1126622

Chemical selection of mutants that affect alcohol dehydrogenase in Drosophila. II. Use of 1-pentyne-3-ol.

J O'Donnell, L Gerace, F Leister, W Sofer.   

Abstract

We describe a procedure for the selection of alcohol dehyrogenase negative mutants in Drosophila. The method consists of exposing eggs and larvae to low concentrations of 1-pentyne-3-ol dissolved in the culture medium. Only those flies with greatly reduced levels of alcohol dehydrogenase activity survive. In addition, genotypically negative flies die if their mothers are alcohol dehydrogenase positive. Using this procedure and formaldehyde to generate mutants, we were able to detect seven alcohol dehydrogenase negative mutants out of 350,000 individuals subjected to selection. At least five of the mutants contain small deletions that include the alcohol dehydrogenase locus.

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Year:  1975        PMID： 1126622      PMCID： PMC1213261     

Source DB:  PubMed          Journal:  Genetics        ISSN： 0016-6731            Impact factor:   4.562


  3 in total

Review 1.  Mutation in eukaryotes.

Authors:  C Auerbach; B J Kilbey
Journal:  Annu Rev Genet       Date:  1971       Impact factor: 16.830

2.  Drosophila alcohol dehydrogenase. Purification and partial characterization.

Authors:  W Sofer; H Ursprung
Journal:  J Biol Chem       Date:  1968-06-10       Impact factor: 5.157

3.  Chemical selection of alcohol dehydrogenase negative mutants in drosophila.

Authors:  W H Sofer; M A Hatkoff
Journal:  Genetics       Date:  1972-11       Impact factor: 4.562
  3 in total
  34 in total

1.  The nucleotide sequence of phenylalanine tRNA2 of Drosophila melanogaster: four isoacceptors with one basic sequence.

Authors:  M Altwegg; E Kubli
Journal:  Nucleic Acids Res       Date:  1979-09-11       Impact factor: 16.971

2.  Structural analysis of the ADHS electromorph of Drosophila melanogaster.

Authors:  T S Fletcher; F J Ayala; D R Thatcher; G K Chambers
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

3.  A simple method for the isolation of allelic series using male-linked translocations.

Authors:  A S Robinson; M E Riva
Journal:  Theor Appl Genet       Date:  1984-02       Impact factor: 5.699

4.  A comparative study of embryonic gene expression inDrosophila andEphestia.

Authors:  Friedrich Leibenguth; Elisabeth Rammo; Rudolf Dubiczky
Journal:  Wilehm Roux Arch Dev Biol       Date:  1979-03

5.  Molecular consequences of two formaldehyde-induced mutations in the alcohol dehydrogenase gene of Drosophila melanogaster.

Authors:  A R Place; C Benyajati; W Sofer
Journal:  Biochem Genet       Date:  1987-10       Impact factor: 1.890

6.  Conversion and reciprocal exchange between tandem repeats in Drosophila melanogaster.

Authors:  R Hipeau-Jacquotte; D L Brutlag; F Brégégère
Journal:  Mol Gen Genet       Date:  1989-12

7.  Larval behavioral response to environmental ethanol in relation to alcohol dehydrogenase activity level in Drosophila melanogaster.

Authors:  E Depiereux; N Hougouto; J Lechien; M Libion-Mannaert; M C Liétaert; E Feytmans; A Elens
Journal:  Behav Genet       Date:  1985-03       Impact factor: 2.805

8.  Nucleotide sequence comparison of the Adh gene in three drosophilids.

Authors:  V H Cohn; M A Thompson; G P Moore
Journal:  J Mol Evol       Date:  1984       Impact factor: 2.395

9.  Chemical selection of mutants that affect ADH activity in Drosophila. III. Effects of ethanol.

Authors:  C Vigue; W Sofer
Journal:  Biochem Genet       Date:  1976-02       Impact factor: 1.890

10.  Alcohol dehydrogenase mutants of Chinese hamster somatic cells resistant to allyl alcohol.

Authors:  J P Thirion; B Talbot
Journal:  Genetics       Date:  1978-02       Impact factor: 4.562
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