Literature DB >> 3778423

Characterization of alcohol dehydrogenase in young soybean seedlings.

R Brzezinski, B G Talbot, D Brown, D Klimuszko, S D Blakeley, J P Thirion.   

Abstract

Molecular properties of alcohol dehydrogenase (ADH) were examined in young soybean seedlings. Soybean radicle tissue is ADH-rich. Enzyme specific activity decreases slowly with the development of roots and becomes almost undetectable when the first true leaves appear. Soybean ADH was not found to be inducible by flooding. 2,4-Dichlorophenoxyacetic acid (2,4-D) treatment increased ADH specific activity as much as 14-fold. Only one ADH isozyme was detected by isoelectric focusing. By DNA-DNA hydridization, soybean ADH genomic sequences were shown to be partly homologous to maize ADH1 cDNA. The presence of more than one Adh gene in soybean is discussed.

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Year:  1986        PMID: 3778423     DOI: 10.1007/bf00498999

Source DB:  PubMed          Journal:  Biochem Genet        ISSN: 0006-2928            Impact factor:   1.890


  21 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

Review 3.  Maize Adh1.

Authors:  M Freeling; D C Bennett
Journal:  Annu Rev Genet       Date:  1985       Impact factor: 16.830

4.  Simultaneous induction by anaerobiosis or 2,4-D of multiple enzymes specificed by two unlinked genes: differential Adh1-Adh2 expression in maize.

Authors:  M Freeling
Journal:  Mol Gen Genet       Date:  1973-12-31

5.  Purification and properties of two distinct groups of ADH isozymes from Chinese hamster liver.

Authors:  B G Talbot; A A Qureshi; R Cohen; J P Thirion
Journal:  Biochem Genet       Date:  1981-08       Impact factor: 1.890

6.  The anaerobic proteins of maize.

Authors:  M M Sachs; M Freeling; R Okimoto
Journal:  Cell       Date:  1980-07       Impact factor: 41.582

7.  In vitro translation of maize ADH: evidence for the anaerobic induction of mRNA.

Authors:  R J Ferl; M D Brennan; D Schwartz
Journal:  Biochem Genet       Date:  1980-08       Impact factor: 1.890

8.  Thiourea: the antioxidant of choice for the purification of proteins from phenol-rich plant tissues.

Authors:  E Van Driessche; S Beeckmans; R Dejaegere; L Kanarek
Journal:  Anal Biochem       Date:  1984-08-15       Impact factor: 3.365

9.  Effects of O2 stress on tomato alcohol dehydrogenase activity: description of a second ADH coding genes.

Authors:  S D Tanksley; R A Jones
Journal:  Biochem Genet       Date:  1981-04       Impact factor: 1.890

10.  Polymorphism of alcohol dehydrogenase in Arabidopsis thaliana (L.) Heynh.: genetical and biochemical characterization.

Authors:  R Dolferus; M Jacobs
Journal:  Biochem Genet       Date:  1984-10       Impact factor: 1.890

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  4 in total

1.  Anaerobic metabolism in plants.

Authors:  R A Kennedy; M E Rumpho; T C Fox
Journal:  Plant Physiol       Date:  1992-09       Impact factor: 8.340

2.  The anaerobic response of soybean.

Authors:  D A Russell; D M Wong; M M Sachs
Journal:  Plant Physiol       Date:  1990-02       Impact factor: 8.340

3.  Molecular characterization of the soybean alcohol dehydrogenase gene family amplified in vitro by the polymerase chain reaction.

Authors:  K D Newman; T T Vantoai
Journal:  Plant Physiol       Date:  1992-09       Impact factor: 8.340

4.  Gene regulation during anaerobiosis in soya roots.

Authors:  K Tihanyi; A Fontanell; J P Thirion
Journal:  Biochem Genet       Date:  1989-12       Impact factor: 1.890

  4 in total

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