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Literature DB >> 6394957

A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.

Abstract

Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

Entities: Chemical Gene Species

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Year: 1984 PMID： 6394957 DOI： 10.1007/BF00330984

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

10 in total

1. Genetic and physiological characterization of met15 mutants of Saccharomyces cerevisiae: a selective system for forward and reverse mutations.

Authors: A Singh; F Sherman
Journal: Genetics Date: 1975-09 Impact factor: 4.562

2. Direct selective techniques for the isolation of pyrimidine auxotrophs in yeast.

Authors: M L Bach; F Lacroute
Journal: Mol Gen Genet Date: 1972

3. Selection of lys2 Mutants of the Yeast SACCHAROMYCES CEREVISIAE by the Utilization of alpha-AMINOADIPATE.

Authors: B B Chattoo; F Sherman; D A Azubalis; T A Fjellstedt; D Mehnert; M Ogur
Journal: Genetics Date: 1979-09 Impact factor: 4.562

4. Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae.

Authors: F Winston; D T Chaleff; B Valent; G R Fink
Journal: Genetics Date: 1984-06 Impact factor: 4.562

5. Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene.

Authors: M Rose; F Winston
Journal: Mol Gen Genet Date: 1984

6. Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli.

Authors: M L Bach; F Lacroute; D Botstein
Journal: Proc Natl Acad Sci U S A Date: 1979-01 Impact factor: 11.205

7. Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae.

Authors: R Jund; F Lacroute
Journal: J Bacteriol Date: 1972-01 Impact factor: 3.490

8. Structure and function of the yeast URA3 gene: expression in Escherichia coli.

Authors: M Rose; P Grisafi; D Botstein
Journal: Gene Date: 1984 Jul-Aug Impact factor: 3.688

9. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.

Authors: D Botstein; S C Falco; S E Stewart; M Brennan; S Scherer; D T Stinchcomb; K Struhl; R W Davis
Journal: Gene Date: 1979-12 Impact factor: 3.688

10. Characteristics and relationships of mercury-resistant mutants and methionine auxotrophs of yeast.

Authors: A Singh; F Sherman
Journal: J Bacteriol Date: 1974-06 Impact factor: 3.490

10 in total

1149 in total

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Authors: C E Guerra; D B Kaback
Journal: Genetics Date: 1999-12 Impact factor: 4.562

2. A genetic strategy to eliminate self-activator baits prior to high-throughput yeast two-hybrid screens.

Authors: A J Walhout; M Vidal
Journal: Genome Res Date: 1999-11 Impact factor: 9.043

3. An activation-independent role of transcription factors in insulator function.

Authors: G Fourel; C Boscheron; E Revardel; E Lebrun; Y F Hu; K C Simmen; K Müller; R Li; N Mermod; E Gilson
Journal: EMBO Rep Date: 2001-02 Impact factor: 8.807

4. Identification of domains and residues within the epsilon subunit of eukaryotic translation initiation factor 2B (eIF2Bepsilon) required for guanine nucleotide exchange reveals a novel activation function promoted by eIF2B complex formation.

Authors: E Gomez; G D Pavitt
Journal: Mol Cell Biol Date: 2000-06 Impact factor: 4.272