Literature DB >> 395030

Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.

D Botstein, S C Falco, S E Stewart, M Brennan, S Scherer, D T Stinchcomb, K Struhl, R W Davis.   

Abstract

A system of biological containment for recombinant DNA experiments in Saccharomyces cerevisiae (Brewer's/Baker's yeast) is described. The principle of containment is sterility: the haploid host strains all contain a mating-type-non-specific sterile mutation. The hosts also contain four auxotrophic mutations suitable for selection for the various kinds of vectors used. All vectors are derivatives of pBR322 which can be selected and maintained in both yeast and Escherichia coli. The system has recently been certified at the HV2 level by the National Institutes of Health.

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Year:  1979        PMID： 395030     DOI： 10.1016/0378-1119(79)90004-0

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  442 in total

1.  The tRNA-binding moiety in GCN2 contains a dimerization domain that interacts with the kinase domain and is required for tRNA binding and kinase activation.

Authors:  H Qiu; J Dong; C Hu; C S Francklyn; A G Hinnebusch
Journal:  EMBO J       Date:  2001-03-15       Impact factor: 11.598

2.  Separate information required for nuclear and subnuclear localization: additional complexity in localizing an enzyme shared by mitochondria and nuclei.

Authors:  A M Rose; P B Joyce; A K Hopper; N C Martin
Journal:  Mol Cell Biol       Date:  1992-12       Impact factor: 4.272

3.  N-terminal mutations modulate yeast SNF1 protein kinase function.

Authors:  F Estruch; M A Treitel; X Yang; M Carlson
Journal:  Genetics       Date:  1992-11       Impact factor: 4.562

4.  The SNF5 protein of Saccharomyces cerevisiae is a glutamine- and proline-rich transcriptional activator that affects expression of a broad spectrum of genes.

Authors:  B C Laurent; M A Treitel; M Carlson
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

5.  Functional analysis of a duplicated linked pair of ribosomal protein genes in Saccharomyces cerevisiae.

Authors:  D M Donovan; M P Remington; D A Stewart; J C Crouse; D J Miles; N J Pearson
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

6.  Properties of a Saccharomyces cerevisiae mtDNA segment conferring high-frequency yeast transformation.

Authors:  B C Hyman; J H Cramer; R H Rownd
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

7.  Nucleotide sequence of the SUF2 frameshift suppressor gene of Saccharomyces cerevisiae.

Authors:  C M Cummins; T F Donahue; M R Culbertson
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

8.  Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies.

Authors:  E Apolinario; M Nocero; M Jin; C S Hoffman
Journal:  Curr Genet       Date:  1993-12       Impact factor: 3.886

9.  Temperature-sensitive lethal mutations on yeast chromosome I appear to define only a small number of genes.

Authors:  D B Kaback; P W Oeller; H Yde Steensma; J Hirschman; D Ruezinsky; K G Coleman; J R Pringle
Journal:  Genetics       Date:  1984-09       Impact factor: 4.562

10.  High-resolution mapping of DNase I-hypersensitive sites of Drosophila heat shock genes in Drosophila melanogaster and Saccharomyces cerevisiae.

Authors:  N Costlow; J T Lis
Journal:  Mol Cell Biol       Date:  1984-09       Impact factor: 4.272
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