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Literature DB >> 6323928

Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene.

Abstract

Insertion mutations in yeast caused by the transposable element Ty have been identified at several genetic loci. In all cases so far, the site of Ty insertion has been in the 5' non-coding region of the affected gene. Experiments presented here demonstrate that the ura3-52 mutation, a non-reverting ura3 mutation, is caused by a Ty insertion mutation within the coding region of the URA3 gene. This Ty insertion within a gene has a similar structure to those in non-coding regions.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1984 PMID： 6323928 DOI： 10.1007/bf00382100

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

7 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast.

Authors: T F Donahue; R S Daves; G Lucchini; G R Fink
Journal: Cell Date: 1983-01 Impact factor: 41.582

3. Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication.

Authors: P J Farabaugh; G R Fink
Journal: Nature Date: 1980-07-24 Impact factor: 49.962

4. DNA rearrangements associated with a transposable element in yeast.

Authors: G S Roeder; G R Fink
Journal: Cell Date: 1980-08 Impact factor: 41.582

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

6. The origins of gene instability in yeast.

Authors: G S Roeder; P J Farabaugh; D T Chaleff; G R Fink
Journal: Science Date: 1980-09-19 Impact factor: 47.728

7. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.

Authors: D Botstein; S C Falco; S E Stewart; M Brennan; S Scherer; D T Stinchcomb; K Struhl; R W Davis
Journal: Gene Date: 1979-12 Impact factor: 3.688

7 in total

72 in total

8. Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.

Authors: G Simchen; F Winston; C A Styles; G R Fink
Journal: Proc Natl Acad Sci U S A Date: 1984-04 Impact factor: 11.205

9. Efficient UV stimulation of yeast integrative transformation requires damage on both plasmid strands.

Authors: M Ninković; M Alacević; F Fabre; Z Zgaga
Journal: Mol Gen Genet Date: 1994-05-10

10. 3'-->5' exonucleases of DNA polymerases epsilon and delta correct base analog induced DNA replication errors on opposite DNA strands in Saccharomyces cerevisiae.

Authors: P V Shcherbakova; Y I Pavlov
Journal: Genetics Date: 1996-03 Impact factor: 4.562

Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast.

3. Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication.

4. DNA rearrangements associated with a transposable element in yeast.

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

6. The origins of gene instability in yeast.

7. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.

1. Discovery of mutations in Saccharomyces cerevisiae by pooled linkage analysis and whole-genome sequencing.

2. Formation of boundaries of transcriptionally silent chromatin by nucleosome-excluding structures.

3. Elongation factor EF-1 alpha gene dosage alters translational fidelity in Saccharomyces cerevisiae.

4. Transposon insertion site profiling chip (TIP-chip).

5. In vivo DNA expression of functional brome mosaic virus RNA replicons in Saccharomyces cerevisiae.

6. Ime1 and Ime2 are required for pseudohyphal growth of Saccharomyces cerevisiae on nonfermentable carbon sources.

7. Analysis of yeast retrotransposon Ty insertions at the CAN1 locus.

8. Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.

9. Efficient UV stimulation of yeast integrative transformation requires damage on both plasmid strands.

10. 3'-->5' exonucleases of DNA polymerases epsilon and delta correct base analog induced DNA replication errors on opposite DNA strands in Saccharomyces cerevisiae.