Literature DB >> 6345581

Simple method for preparation of antibody-enzyme conjugates for enzyme-linked immunosorbent assays.

P J De Jong.   

Abstract

Mixing suspensions of rabbit antibody for staphylococcal enterotoxin A and commercial anti-rabbit immunoglobulin G-alkaline phosphatase conjugate produced a complex conjugate suitable for use as the second antibody in a conventional enzyme-linked immunosorbent assay. The method was useful for detecting 1 ng of staphylococcal enterotoxin A per ml.

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Year:  1983        PMID： 6345581      PMCID： PMC272769          DOI： 10.1128/jcm.17.5.928-930.1983

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD.

Authors:  E P CASMAN; R W BENNETT
Journal:  Appl Microbiol       Date:  1965-03

2.  Simple assay for staphylococcal enterotoxins A, B, and C: modification of enzyme-linked immunosorbent assay.

Authors:  G Stiffler-Rosenberg; H Fey
Journal:  J Clin Microbiol       Date:  1978-11       Impact factor: 5.948

3.  Homogeneous enzyme immune assay for staphylococcal enterotoxin B.

Authors:  T N Morita; M J Woodburn
Journal:  Infect Immun       Date:  1978-08       Impact factor: 3.441

4.  Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A.

Authors:  G C Saunders; M L Bartlett
Journal:  Appl Environ Microbiol       Date:  1977-11       Impact factor: 4.792

5.  Detection of staphylococcal enterotoxin in foods.

Authors:  R Reiser; D Conaway; M S Bergdoll
Journal:  Appl Microbiol       Date:  1974-01

6.  Enzyme immunoassay for staphylococcal enterotoxin A.

Authors:  P E Kauffman
Journal:  J Assoc Off Anal Chem       Date:  1980-09

7.  A sandwich ELISA method for detection of Staphylococcus aureus enterotoxins.

Authors:  B P Berdal; O Olsvik; T Omland
Journal:  Acta Pathol Microbiol Scand B       Date:  1981-12
  7 in total
  1 in total

1.  Enzyme-linked immunosorbent assays for detection of thermostable direct hemolysin of Vibrio parahaemolyticus.

Authors:  T Honda; M Yoh; U Kongmuang; T Miwatani
Journal:  J Clin Microbiol       Date:  1985-09       Impact factor: 5.948
  1 in total

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