Literature DB >> 4044797

Enzyme-linked immunosorbent assays for detection of thermostable direct hemolysin of Vibrio parahaemolyticus.

T Honda, M Yoh, U Kongmuang, T Miwatani.   

Abstract

Several systems for enzyme-linked immunosorbent assay (ELISA) of thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus were tested, and single-antibody sandwich ELISA systems gave satisfactory results. ELISA was able to detect as little as several nanograms of purified TDH per milliliter. The method of De Jong (J. Clin. Microbiol. 17:928-930, 1983) and the glutaraldehyde method were successful for preparing conjugates of alkaline phosphatase and anti-TDH antibody. TDH in fluids in intestinal loops of experimental animals challenged with living V. parahaemolyticus was accurately detectable by ELISA.

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Year:  1985        PMID： 4044797      PMCID： PMC268415          DOI： 10.1128/jcm.22.3.383-386.1985

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Inhibition of hemolytic activity of the thermostable direct hemolysin of Vibrio parahaemolyticus by ganglioside.

Authors:  R Takeda; T Honda; J Sakural; N Otomo
Journal:  Infect Immun       Date:  1975-10       Impact factor: 3.441

2.  Inactivation of the biological activities of the thermostable direct hemolysin of Vibrio parahaemolyticus by ganglioside Gt1.

Authors:  Y Takeda; T Takeda; T Honda; T Miwatani
Journal:  Infect Immun       Date:  1976-07       Impact factor: 3.441

3.  Proposal of Vibrio alginolyticus for the biotype 2 of Vibrio parahaemolyticus.

Authors:  R Sakazaki
Journal:  Jpn J Med Sci Biol       Date:  1968-10

4.  In vitro hemolytic characteristic of Vibrio parahaemolyticus: its close correlation with human pathogenicity.

Authors:  Y Miyamoto; T Kato; Y Obara; S Akiyama; K Takizawa; S Yamai
Journal:  J Bacteriol       Date:  1969-11       Impact factor: 3.490

5.  [Development of a sensitive serological assay based on reversed passive hemagglutination for detection of enteropathogenic toxin (kanagawa hemolysin) of Vibrio parahaemolyticus, and re-evaluation of the toxin producibility of isolates from various sources (author's transl)].

Authors:  K Ohta; Y Kudoh; M Tsuno; S Sakai; T Maruyama; T Itoh; M Ohashi
Journal:  Nihon Saikingaku Zasshi       Date:  1979-11

6.  Demonstration of the cardiotoxicity of the thermostable direct hemolysin (lethal toxin) produced by Vibrio parahaemolyticus.

Authors:  T Honda; K Goshima; Y Takeda; Y Sugino; T Miwatani
Journal:  Infect Immun       Date:  1976-01       Impact factor: 3.441

7.  Identification of lethal toxin with the thermostable direct hemolysin produced by Vibrio parahaemolyticus, and some physicochemical properties of the purified toxin.

Authors:  T Honda; S Taga; T Takeda; M A Hasibuan; Y Takeda; T Miwatani
Journal:  Infect Immun       Date:  1976-01       Impact factor: 3.441

8.  Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.

Authors:  R H Yolken; H B Greenberg; M H Merson; R B Sack; A Z Kapikian
Journal:  J Clin Microbiol       Date:  1977-11       Impact factor: 5.948

9.  Immunological methods for detection of Kanagawa phenomenon of Vibrio parahaemolyticus.

Authors:  T Honda; S Chearskul; Y Takeda; T Miwatani
Journal:  J Clin Microbiol       Date:  1980-06       Impact factor: 5.948

10.  Simplified purification and biophysicochemical characteristics of Kanagawa phenomenon-associated hemolysin of Vibrio parahaemolyticus.

Authors:  Y Miyamoto; Y Obara; T Nikkawa; S Yamai; T Kato; Y Yamada; M Ohashi
Journal:  Infect Immun       Date:  1980-05       Impact factor: 3.441
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  10 in total

1.  Production and characterization of monoclonal antibodies to a pilus colonization factor (colonization factor antigen III) of human enterotoxigenic Escherichia coli.

Authors:  T Honda; N Wetprasit; M Arita; T Miwatani
Journal:  Infect Immun       Date:  1989-11       Impact factor: 3.441

2.  Molecular epidemiologic evidence for association of thermostable direct hemolysin (TDH) and TDH-related hemolysin of Vibrio parahaemolyticus with gastroenteritis.

Authors:  H Shirai; H Ito; T Hirayama; Y Nakamoto; N Nakabayashi; K Kumagai; Y Takeda; M Nishibuchi
Journal:  Infect Immun       Date:  1990-11       Impact factor: 3.441

Review 3.  Fungal hemolysins.

Authors:  Ajay P Nayak; Brett J Green; Donald H Beezhold
Journal:  Med Mycol       Date:  2012-07-09       Impact factor: 4.076

4.  Development and evaluation of a rapid, simple, and sensitive immunochromatographic assay to detect thermostable direct hemolysin produced by Vibrio parahaemolyticus in enrichment cultures of stool specimens.

Authors:  Kentaro Kawatsu; Masanori Ishibashi; Teizo Tsukamoto
Journal:  J Clin Microbiol       Date:  2006-05       Impact factor: 5.948

5.  Enzyme-linked immunosorbent assay to detect Shiga toxin of Shigella dysenteriae and related toxins.

Authors:  U Kongmuang; T Honda; T Miwatani
Journal:  J Clin Microbiol       Date:  1987-01       Impact factor: 5.948

6.  Demonstration of a plasmid-borne gene encoding a thermostable direct hemolysin in Vibrio cholerae non-O1 strains.

Authors:  T Honda; M Nishibuchi; T Miwatani; J B Kaper
Journal:  Appl Environ Microbiol       Date:  1986-11       Impact factor: 4.792

7.  Production of monoclonal antibodies against thermostable direct hemolysin of Vibrio parahaemolyticus and application of the antibodies for enzyme-linked immunosorbent assay.

Authors:  T Honda; Y Ni; M Yoh; T Miwatani
Journal:  Med Microbiol Immunol       Date:  1989       Impact factor: 3.402

8.  Purification and partial characterization of a non-O1 Vibrio cholerae hemolysin that cross-reacts with thermostable direct hemolysin of Vibrio parahaemolyticus.

Authors:  M Yoh; T Honda; T Miwatani
Journal:  Infect Immun       Date:  1986-04       Impact factor: 3.441

9.  Synthetic oligodeoxyribonucleotide probes to detect Kanagawa phenomenon-positive Vibrio parahaemolyticus.

Authors:  M Nishibuchi; W E Hill; G Zon; W L Payne; J B Kaper
Journal:  J Clin Microbiol       Date:  1986-06       Impact factor: 5.948

10.  Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin.

Authors:  T Honda; Y X Ni; T Miwatani
Journal:  Infect Immun       Date:  1988-04       Impact factor: 3.441
  10 in total

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