Literature DB >> 337898

Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A.

G C Saunders, M L Bartlett.   

Abstract

A simple double-antibody enzyme immunoassay that uses a microtechnique was developed for detecting staphylococcal enterotoxin A in food products. Sample preparation can be completed in less than 15 min. Assay sensitivity ranges from 0.4 ng (20-h test time) to 3.2 ng (1- to 3-h test time) of toxin per ml of prepared sample. Separation and detection of enterotoxin from spiked food products ranged between 72 and 98% of the amount added.

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Year:  1977        PMID： 337898      PMCID： PMC242693          DOI： 10.1128/aem.34.5.518-522.1977

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  Immunoassay using antigen-enzyme conjugates.

Authors:  B K. Van Weemen; A H.W.M. Schuurs
Journal:  FEBS Lett       Date:  1971-06-24       Impact factor: 4.124

2.  DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD.

Authors:  E P CASMAN; R W BENNETT
Journal:  Appl Microbiol       Date:  1965-03

3.  Development and evaluation of an enzyme-labeled antibody test for the rapid detection of hog cholera antibodies.

Authors:  G C Saunders
Journal:  Am J Vet Res       Date:  1977-01       Impact factor: 1.156

4.  Peroxidase-labeled antibody. A new method of conjugation.

Authors:  P K Nakane; A Kawaoi
Journal:  J Histochem Cytochem       Date:  1974-12       Impact factor: 2.479

5.  Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.

Authors:  H M Johnson; J A Bukovic; P E Kauffmann
Journal:  Appl Microbiol       Date:  1973-09

6.  Rapid, sensitive assay for staphylococcal enterotoxin and a comparison of serological methods.

Authors:  S J Silverman; A R Knott; M Howard
Journal:  Appl Microbiol       Date:  1968-07

7.  Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test.

Authors:  G C Saunders; E H Clinard
Journal:  J Clin Microbiol       Date:  1976-06       Impact factor: 5.948

8.  Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.

Authors:  G Wolters; L Kuijpers; J Kacaki; A Schuurs
Journal:  J Clin Pathol       Date:  1976-10       Impact factor: 3.411

9.  A rapid solid phase radioimmunoassay for staphylococcal B enterotoxin.

Authors:  W S Collins; J F Metzger; A D Johnson
Journal:  J Immunol       Date:  1972-03       Impact factor: 5.422

10.  Recovery of staphylococcal enterotoxin from foods by affinity chromatography.

Authors:  C Genigeorgis; J K Kuo
Journal:  Appl Environ Microbiol       Date:  1976-02       Impact factor: 4.792
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  23 in total

1.  Rapid and sensitive sandwich enzyme-linked immunosorbent assay for detection of staphylococcal enterotoxin B in cheese.

Authors:  C Morissette; J Goulet; G Lamoureux
Journal:  Appl Environ Microbiol       Date:  1991-03       Impact factor: 4.792

2.  Simple assay for staphylococcal enterotoxins A, B, and C: modification of enzyme-linked immunosorbent assay.

Authors:  G Stiffler-Rosenberg; H Fey
Journal:  J Clin Microbiol       Date:  1978-11       Impact factor: 5.948

3.  Homogeneous enzyme immune assay for staphylococcal enterotoxin B.

Authors:  T N Morita; M J Woodburn
Journal:  Infect Immun       Date:  1978-08       Impact factor: 3.441

4.  Rapid latex agglutination test for detection of staphylococcal enterotoxins A to E that uses high-density latex particles.

Authors:  H Fujikawa; H Igarashi
Journal:  Appl Environ Microbiol       Date:  1988-10       Impact factor: 4.792

5.  Competitive, enzyme-linked immunosorbent assay for toxic shock syndrome toxin 1.

Authors:  J Parsonnet; J T Mills; Z A Gillis; G B Pier
Journal:  J Clin Microbiol       Date:  1985-07       Impact factor: 5.948

6.  The use of a sandwich ELISA for the detection of staphylococcal enterotoxin A in foods from outbreaks of food poisoning.

Authors:  A A Wieneke; R J Gilbert
Journal:  J Hyg (Lond)       Date:  1985-08

7.  A class capture enzyme immunoassay for immunoglobulin level determinations in bovine sera.

Authors:  K Nielsen; B Rosenbaum; J Stiller
Journal:  Can J Comp Med       Date:  1985-10

8.  Direct skin test in highly sensitized guinea pigs for rapid and sensitive determination of staphylococcal enterotoxin B.

Authors:  P H Scheuber; H Mossmann; G Beck; D K Hammer
Journal:  Appl Environ Microbiol       Date:  1983-12       Impact factor: 4.792

9.  Enterotoxigenicity of Staphylococcus aureus strains from clinical isolates.

Authors:  G Mauff; I Röhrig; U Ernzer; W Lenz; M Bergdoll; G Pulverer
Journal:  Eur J Clin Microbiol       Date:  1983-08       Impact factor: 3.267

10.  Comparative evaluation of different enzyme-linked immunosorbent assay systems for the detection of staphylococcal enterotoxins A, B, C, and D.

Authors:  H Fey; H Pfister; O Rüegg
Journal:  J Clin Microbiol       Date:  1984-01       Impact factor: 5.948
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