Literature DB >> 6332307

The B----Z transition in two synthetic oligonucleotides: d(C-2-amino-ACGTG) and d(m5CGCAm5CGTGCG) studied by IR, NMR and CD spectroscopies.

J A Taboury, S Adam, E Taillandier, J M Neumann, S Tran-Dinh, T Huynh-Dinh, B Langlois d'Estaintot, M Conti, J Igolen.   

Abstract

The sequences CA'CGTG (where A' = 2-aminodeoxyadenosine) and m5CGCAm5CGTGCG are prepared and studied by IR, CD and 1H-NMR. Infrared spectra demonstrate the capacity of the modified hexamer and decamer to adopt a Z conformation. The influence of the NH2 substitution on the adenine or of the methylated terminal part of the decamer acting with the increase of the DNA concentration stabilizes the Z conformation at room temperature in low humidity films. Very weak proportion of Z conformation is detected in UV dilute solutions. In more concentrated NMR solutions, the Z proportion induced by high salt content is only 20-25%. The effects of the concentration and of the covalent modification of the bases are discussed.

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Year:  1984        PMID： 6332307      PMCID： PMC320073          DOI： 10.1093/nar/12.15.6291

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  Salt-induced co-operative conformational change of a synthetic DNA: equilibrium and kinetic studies with poly (dG-dC).

Authors:  F M Pohl; T M Jovin
Journal:  J Mol Biol       Date:  1972-06-28       Impact factor: 5.469

2.  Molecular structure of a left-handed double helical DNA fragment at atomic resolution.

Authors:  A H Wang; G J Quigley; F J Kolpak; J L Crawford; J H van Boom; G van der Marel; A Rich
Journal:  Nature       Date:  1979-12-13       Impact factor: 49.962

3.  Structural transitions in DNAs and nucleohistones studied by I.R. spectroscopy.

Authors:  E Taillandier; J Taboury; J Liquier; P Sautière; M Couppez
Journal:  Biochimie       Date:  1981 Nov-Dec       Impact factor: 4.079

4.  High-salt d(CpGpCpG), a left-handed Z' DNA double helix.

Authors:  H Drew; T Takano; S Tanaka; K Itakura; R E Dickerson
Journal:  Nature       Date:  1980-08-07       Impact factor: 49.962

5.  Nature of side-reactions in oligonucleotide synthesis involving arenesulphonyl derivatives of 3-nitro-1,2,4-triazole and related condensing agents.

Authors:  C B Reese; A Ubasawa
Journal:  Nucleic Acids Symp Ser       Date:  1980

6.  Left-handed double helical DNA: variations in the backbone conformation.

Authors:  A J Wang; G J Quigley; F J Kolpak; G van der Marel; J H van Boom; A Rich
Journal:  Science       Date:  1981-01-09       Impact factor: 47.728

7.  Comparison of poly(dG-dC).poly(dG-dC) conformations in oriented films and in solution.

Authors:  J Pilet; M Leng
Journal:  Proc Natl Acad Sci U S A       Date:  1982-01       Impact factor: 11.205

8.  The high salt form of poly(dG-dC).poly(dG-dC) is left-handed Z-DNA: Raman spectra of crystals and solutions.

Authors:  T J Thamann; R C Lord; A H Wang; A Rich
Journal:  Nucleic Acids Res       Date:  1981-10-24       Impact factor: 16.971

9.  B,Z conformations and mechanism of the Z-B-coil transitions of the self-complementary deoxy-hexanucleotide d(C-G-m5C-G-C-G) by 1H-NMR and CD spectroscopy.

Authors:  J A Cavailles; J M Neumann; J Taboury; B Langlois d'Estaintot; T Huynh-Dinh; J Igolen; S Tran-Dinh
Journal:  J Biomol Struct Dyn       Date:  1984-06

10.  Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).

Authors:  M Behe; G Felsenfeld
Journal:  Proc Natl Acad Sci U S A       Date:  1981-03       Impact factor: 11.205
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  8 in total

1.  DNA containing the base analogue 2-aminoadenine: preparation, use as hybridization probes and cleavage by restriction endonucleases.

Authors:  A Chollet; E Kawashima
Journal:  Nucleic Acids Res       Date:  1988-01-11       Impact factor: 16.971

2.  Conformational flexibility in the right-handed and in the left-handed double helices of Na+-d(m5C-G-T-A-m5C-G) studied by IR spectroscopy.

Authors:  E Taillandier; S Adam; J P Ridoux; J Liquier
Journal:  Nucleic Acids Res       Date:  1988-06-24       Impact factor: 16.971

3.  Right-handed and left-handed helices of poly(dA-dC) X (dG-dT).

Authors:  J A Taboury; E Taillandier
Journal:  Nucleic Acids Res       Date:  1985-06-25       Impact factor: 16.971

4.  Z form of poly d(A-C).poly d(G-T) in solution studied by Raman spectroscopy.

Authors:  J P Ridoux; J Liquier; E Taillandier
Journal:  Nucleic Acids Res       Date:  1987-07-24       Impact factor: 16.971

5.  Modified oligonucleotides as alternatives to the synthesis of mixed probes for the screening of cDNA libraries.

Authors:  T Huynh-Dinh; N Duchange; M M Zakin; A Lemarchand; J Igolen
Journal:  Proc Natl Acad Sci U S A       Date:  1985-11       Impact factor: 11.205

6.  Influence of uracil defect on DNA structure: 1H NMR investigation at 500 MHz.

Authors:  A M Delort; J M Neumann; D Molko; M Hervé; R Téoule; S Tran Dinh
Journal:  Nucleic Acids Res       Date:  1985-05-10       Impact factor: 16.971

7.  NMR, CD and IR spectroscopies of a tridecanucleotide containing a no-base residue: coexistence of B and Z conformations.

Authors:  S Pochet; T Huynh-Dinh; J M Neumann; S Tran-Dinh; S Adam; J Taboury; E Taillandier; J Igolen
Journal:  Nucleic Acids Res       Date:  1986-01-24       Impact factor: 16.971

8.  Interruptions of (CG)n sequences by GG, TG and CA need not prevent B to Z transition in solution.

Authors:  R K Mishra; P K Latha; S K Brahmachari
Journal:  Nucleic Acids Res       Date:  1988-05-25       Impact factor: 16.971
  8 in total

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