Literature DB >> 3865174

Modified oligonucleotides as alternatives to the synthesis of mixed probes for the screening of cDNA libraries.

T Huynh-Dinh, N Duchange, M M Zakin, A Lemarchand, J Igolen.   

Abstract

Two simple alternatives to the synthesis of mixed oligodeoxyribonucleotide probes are described: mixed deblocking of triazolodeoxynucleosides for the A/G and C/T degeneracies or incorporation of 2-amino-2'-deoxyadenosines into a determined sequence for a higher stability of the hybridization duplexes. The synthetic oligodeoxyribonucleotides obtained were successfully tested in comparison with a classical mixed probe for their capacity to identify specific cDNA clones of human antithrombin III. The results are discussed with respect to the utilization of these synthetic oligonucleotides as hybridization probes for the isolation of cloned cDNA sequences.

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Year:  1985        PMID： 3865174      PMCID： PMC390846          DOI： 10.1073/pnas.82.22.7510

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

1.  Sequence requirements for nuclear location of simian virus 40 large-T antigen.

Authors:  D Kalderon; W D Richardson; A F Markham; A E Smith
Journal:  Nature       Date:  1984 Sep 6-11       Impact factor: 49.962

2.  Quick-blot: selective mRNA or DNA immobilization from whole cells.

Authors:  J Bresser; J Doering; D Gillespie
Journal:  DNA       Date:  1983

3.  The influence of the purine 2-amino group on DNA conformation and stability. Synthesis and conformational analysis of d[T(2-aminoA)]3.

Authors:  B L Gaffney; L A Marky; R A Jones
Journal:  Nucleic Acids Res       Date:  1982-07-24       Impact factor: 16.971

4.  Use of a cDNA clone for the fourth component of human complement (C4) for analysis of a genetic deficiency of C4 in guinea pig.

Authors:  A S Whitehead; G Goldberger; D E Woods; A F Markham; H R Colten
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

5.  Human apolipoproteins AI, AII, CII and CIII. cDNA sequences and mRNA abundance.

Authors:  C R Sharpe; A Sidoli; C S Shelley; M A Lucero; C C Shoulders; F E Baralle
Journal:  Nucleic Acids Res       Date:  1984-05-11       Impact factor: 16.971

6.  Isolation of a genomic clone for bovine pancreatic trypsin inhibitor by using a unique-sequence synthetic DNA probe.

Authors:  S Anderson; I B Kingston
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

7.  Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.

Authors:  R B Wallace; J Shaffer; R F Murphy; J Bonner; T Hirose; K Itakura
Journal:  Nucleic Acids Res       Date:  1979-08-10       Impact factor: 16.971

8.  Synthesis of 4-triazolopyrimidinone nucleotide and its application in synthesis of 5-methylcytosine-containing oligodeoxyribonucleotides.

Authors:  W L Sung
Journal:  Nucleic Acids Res       Date:  1981-11-25       Impact factor: 16.971

9.  The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.

Authors:  R B Wallace; M J Johnson; T Hirose; T Miyake; E H Kawashima; K Itakura
Journal:  Nucleic Acids Res       Date:  1981-02-25       Impact factor: 16.971

10.  The gene structure of human anti-haemophilic factor IX.

Authors:  D S Anson; K H Choo; D J Rees; F Giannelli; K Gould; J A Huddleston; G G Brownlee
Journal:  EMBO J       Date:  1984-05       Impact factor: 11.598
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  5 in total

1.  5-Fluorodeoxyuridine as an alternative to the synthesis of mixed hybridization probes for the detection of specific gene sequences.

Authors:  J F Habener; C D Vo; D B Le; G P Gryan; L Ercolani; A H Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

2.  The 6-thioguanine/5-methyl-2-pyrimidinone base pair.

Authors:  H P Rappaport
Journal:  Nucleic Acids Res       Date:  1988-08-11       Impact factor: 16.971

3.  Hybridization triggered cross-linking of deoxyoligonucleotides.

Authors:  T R Webb; M D Matteucci
Journal:  Nucleic Acids Res       Date:  1986-10-10       Impact factor: 16.971

4.  Antithrombin III tours gene: identification of a point mutation leading to an arginine----cysteine replacement in a silent deficiency.

Authors:  N Duchange; J F Chassé; G N Cohen; M M Zakin
Journal:  Nucleic Acids Res       Date:  1986-03-11       Impact factor: 16.971

5.  The applications of synthetic oligonucleotides to molecular biology.

Authors:  R W Ellis
Journal:  Pharm Res       Date:  1986-08       Impact factor: 4.200
  5 in total

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