Transcription from SV 40-like monkey DNA sequences.

The ability of an African green monkey genomic segment, homologous to the regulatory region of SV40, to promote transcription in monkey cells has been investigated. Genomic transcripts from CV-1 cells hybridize to both strands of this SV40-like segment. Further examination of the promoter potential of the SV40-like segment using expression vectors suggests that the SV40-like region contains at least part of the necessary information for promoting transcription in both directions. In addition, the latter experiments suggest that sequences several hundred nucleotides away in the genome modulate the transcription initiating at multiple sites in the SV40-like segment.