Literature DB >> 6315402

Easy identification of cDNA clones.

U Rüther, B Müller-Hill.   

Abstract

A set of six cloning vectors, pUR 278, 288, 289, 290, 291, 292 is presented. These vectors have the cloning sites, BamHI, SalI, PstI, XbaI and HindIII, in all frames at the 3' end of the lacZ gene. Insertion of cDNA in the proper cloning sites leads to a fusion protein of active beta-galactosidase and the peptide encoded by the cDNA. A simple immunoenzymatic assay can be used to identify clones in such a cDNA library.

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Year:  1983        PMID： 6315402      PMCID： PMC555360          DOI： 10.1002/j.1460-2075.1983.tb01659.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  22 in total

1.  Exon cloning: immunoenzymatic identification of exons of the chicken lysozyme gene.

Authors:  U Rüther; M Koenen; A E Sippel; B Müller-Hill
Journal:  Proc Natl Acad Sci U S A       Date:  1982-11       Impact factor: 11.205

2.  Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.

Authors:  S V Suggs; R B Wallace; T Hirose; E H Kawashima; K Itakura
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

3.  Expression of antigenic determinants of the hemagglutinin gene of a human influenza virus in Escherichia coli.

Authors:  A R Davis; D P Nayak; M Ueda; A L Hiti; D Dowbenko; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1981-09       Impact factor: 11.205

4.  Efficient isolation of genes by using antibody probes.

Authors:  R A Young; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

5.  pUR222, a vector for cloning and rapid chemical sequencing of DNA.

Authors:  U Rüther; M Koenen; K Otto; B Müller-Hill
Journal:  Nucleic Acids Res       Date:  1981-08-25       Impact factor: 16.971

6.  pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts.

Authors:  U Rüther
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971

7.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors:  J Vieira; J Messing
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

8.  High-efficiency cloning of full-length cDNA.

Authors:  H Okayama; P Berg
Journal:  Mol Cell Biol       Date:  1982-02       Impact factor: 4.272

9.  Characterization and cloning of the mRNAs specific for the lactating mouse mammary gland.

Authors:  L G Hennighausen; A E Sippel
Journal:  Eur J Biochem       Date:  1982-06-15

10.  Direct immunoassay for detecting Escherichia coli colonies that contain polypeptides encoded by cloned DNA segments.

Authors:  D J Kemp; A F Cowman
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205
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  191 in total

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Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

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Authors:  R Allen Helm; Alison G Lee; Harry D Christman; Stanley Maloy
Journal:  Genetics       Date:  2003-11       Impact factor: 4.562

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Journal:  J Virol       Date:  1992-06       Impact factor: 5.103

4.  Development and characterization of a panel of monoclonal antibodies against the catalytic domain of the human fes proto-oncogene product.

Authors:  A van Bokhoven; H L van Duijnhoven; M Jücker; A J Roebroek; W J van de Ven
Journal:  Mol Biol Rep       Date:  1992-02       Impact factor: 2.316

5.  Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2.

Authors:  M Amemura; K Makino; H Shinagawa; A Nakata
Journal:  J Bacteriol       Date:  1990-11       Impact factor: 3.490

6.  Signal transduction by steroid hormones: nuclear localization is differentially regulated in estrogen and glucocorticoid receptors.

Authors:  D Picard; V Kumar; P Chambon; K R Yamamoto
Journal:  Cell Regul       Date:  1990-02

7.  The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.

Authors:  T Sekiguchi; Y Nohiro; Y Nakamura; N Hisamoto; T Nishimoto
Journal:  Mol Cell Biol       Date:  1991-06       Impact factor: 4.272

8.  Lymphoproliferative response to fusion proteins of human papillomaviruses in patients with cervical intraepithelial neoplasia.

Authors:  H A Cubie; M Norval; L Crawford; L Banks; T Crook
Journal:  Epidemiol Infect       Date:  1989-12       Impact factor: 2.451

9.  Abnormal muscle development in the heldup3 mutant of Drosophila melanogaster is caused by a splicing defect affecting selected troponin I isoforms.

Authors:  J A Barbas; J Galceran; L Torroja; A Prado; A Ferrús
Journal:  Mol Cell Biol       Date:  1993-03       Impact factor: 4.272

10.  Cloning and sequencing of the gene for alpha antigen from Mycobacterium avium and mapping of B-cell epitopes.

Authors:  N Ohara; K Matsuo; R Yamaguchi; A Yamazaki; H Tasaka; T Yamada
Journal:  Infect Immun       Date:  1993-04       Impact factor: 3.441
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