Literature DB >> 6184717

Exon cloning: immunoenzymatic identification of exons of the chicken lysozyme gene.

U Rüther, M Koenen, A E Sippel, B Müller-Hill.   

Abstract

A 10-kilobase DNA fragment containing exons 1 and 2 of the chicken lysozyme gene has been randomly cleaved with DNase I. After tailing and cloning into the plasmid pUK230, Lac+ colonies were selected. Colonies harboring expressed fragments of the exons could be detected by an immunoenzymatic assay using antibodies against lysozyme. The smallest fragment coded for 10 amino acids and the largest coded for almost all residues of exon 2. These results suggest that any gene of any genome cloned in this way can be detected if antibodies against the gene product are available.

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Year:  1982        PMID： 6184717      PMCID： PMC347231          DOI： 10.1073/pnas.79.22.6852

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Immunological screening method to detect specific translation products.

Authors:  S Broome; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1978-06       Impact factor: 11.205

3.  Arrangement of coding and intervening sequences of chicken lysozyme gene.

Authors:  W Lindenmaier; M C Nguyen-Huu; R Lurz; M Stratmann; N Blin; T Wurtz; H J Hauser; A E Sippel; G Schütz
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

4.  Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Authors:  J Messing; B Gronenborn; B Müller-Hill; P Hans Hopschneider
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

5.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

6.  Lac repressor can be fused to beta-galactosidase.

Authors:  B Müller-Hill; J Kania
Journal:  Nature       Date:  1974-06-07       Impact factor: 49.962

7.  Cloning of chicken lysozyme structural gene sequences synthesized in vitro.

Authors:  A E Sippel; H Land; W Lindenmaier; M C Nguyen-Huu; T Wurtz; K N Timmis; K Giesecke; G Schütz
Journal:  Nucleic Acids Res       Date:  1978-09       Impact factor: 16.971

8.  Why genes in pieces?

Authors:  W Gilbert
Journal:  Nature       Date:  1978-02-09       Impact factor: 49.962

9.  The precise and entire antigenic structure of native lysozyme.

Authors:  M Z Atassi; C L Lee
Journal:  Biochem J       Date:  1978-05-01       Impact factor: 3.857

10.  Immunoadsorption of specific chicken oviduct polysomes. Isolation of ovalbumin, ovomucoid, and lysozyme messenger RNA.

Authors:  B Groner; N E Hynes; A E Sippel; S Jeep; M Chi-Nguyen-Huu; G Schütz
Journal:  J Biol Chem       Date:  1977-10-10       Impact factor: 5.157
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  17 in total

1.  Cloning of DNA sequences encoding foreign peptides and their expression in the K88 pili.

Authors:  G Thiry; A Clippe; T Scarcez; J Petre
Journal:  Appl Environ Microbiol       Date:  1989-04       Impact factor: 4.792

2.  Use of a foreign epitope as a "tag" for the localization of minor proteins within a cell: the case of the immunity protein to colicin A.

Authors:  V Geli; D Baty; C Lazdunski
Journal:  Proc Natl Acad Sci U S A       Date:  1988-02       Impact factor: 11.205

3.  Characterisation of P. falciparum antigenic determinants isolated from a genomic expression library by differential antibody screening.

Authors:  G Langsley; A Scherf; O Mercereau-Puijalon; M Koenen; B Kahane; D Mattei; M Guillotte; L Sibilli; I Garner; B Müller-Hill
Journal:  Nucleic Acids Res       Date:  1985-06-11       Impact factor: 16.971

4.  Screening for in vivo protein-protein interactions.

Authors:  F J Germino; Z X Wang; S M Weissman
Journal:  Proc Natl Acad Sci U S A       Date:  1993-02-01       Impact factor: 11.205

5.  Method to map antigenic determinants recognized by monoclonal antibodies: localization of a determinant of virus neutralization on the feline leukemia virus envelope protein gp70.

Authors:  J H Nunberg; G Rodgers; J H Gilbert; R M Snead
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

6.  Expression of antigenic regions of a trypanosome variable surface glycoprotein in E. coli using Bal-31 nuclease digestion.

Authors:  M J Lenardo; S T Brentano; J E Donelson
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

7.  Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.

Authors:  J Germino; J G Gray; H Charbonneau; T Vanaman; D Bastia
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

8.  Expression in Escherichia coli of a dominant immunogen of Trypanosoma cruzi recognized by human chagasic sera.

Authors:  P C Cotrim; G S Paranhos; R A Mortara; J Wanderley; A Rassi; M E Camargo; J F da Silveira
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

9.  Surface proteins of Mycoplasma hyopneumoniae identified from an Escherichia coli expression plasmid library.

Authors:  M Q Klinkert; R Herrmann; H Schaller
Journal:  Infect Immun       Date:  1985-08       Impact factor: 3.441

10.  Molecular cloning and expression of Chlamydia trachomatis major outer membrane protein antigens in Escherichia coli.

Authors:  R S Stephens; C C Kuo; G Newport; N Agabian
Journal:  Infect Immun       Date:  1985-03       Impact factor: 3.441
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