Literature DB >> 6170982

Expression of antigenic determinants of the hemagglutinin gene of a human influenza virus in Escherichia coli.

A R Davis, D P Nayak, M Ueda, A L Hiti, D Dowbenko, D G Kleid.   

Abstract

Antigenic determinants of influenza virus hemagglutinin were expressed in Escherichia coli. DNA coding for presequences of hemagglutinin were removed and an ATG codon was placed before DNA coding for mature hemagglutinin. A number of expression plasmids were constructed in which various segments of this reconstructed hemagglutinin DNA were fused to DNA coding for bacterial beta-galactosidase. The fusion proteins exhibited specific binding to antiviral antibodies. This binding could be competitively inhibited by excess viral hemagglutinin, demonstrating that these fusion proteins contained antigenic determinants of hemagglutinin.

Entities:  

Mesh：

Substances：

Year:  1981        PMID： 6170982      PMCID： PMC348748          DOI： 10.1073/pnas.78.9.5376

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

1.  Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril.

Authors:  M A Markwell; C F Fox
Journal:  Biochemistry       Date:  1978-10-31       Impact factor: 3.162

2.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

Review 3.  Structure of the haemagglutinin of influenza virus.

Authors:  M D Waterfield; K Espelie; K Elder; J J Skehel
Journal:  Br Med Bull       Date:  1979-01       Impact factor: 4.291

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Antigenic determinants of influenza virus hemagglutinin. I. Cyanogen bromide peptides derived from A/MEMPHIS/72 hemagglutinin possess antigenic activity.

Authors:  D C Jackson; R J Russell; C W Ward; T A Dopheide
Journal:  Virology       Date:  1978-08       Impact factor: 3.616

6.  Expression in Escherichia coli of chemically synthesized genes for human insulin.

Authors:  D V Goeddel; D G Kleid; F Bolivar; H L Heyneker; D G Yansura; R Crea; T Hirose; A Kraszewski; K Itakura; A D Riggs
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

7.  Complete sequence analysis shows that the hemagglutinins of the H0 and H2 subtypes of human influenza virus are closely related.

Authors:  A L Hiti; A R Davis; D P Nayak
Journal:  Virology       Date:  1981-05       Impact factor: 3.616

8.  Evidence for a ninth influenza viral polypeptide.

Authors:  R A Lamb; P R Etkind; P W Choppin
Journal:  Virology       Date:  1978-11       Impact factor: 3.616

9.  Translation of the leader region of the Escherichia coli tryptophan operon.

Authors:  G F Miozzari; C Yanofsky
Journal:  J Bacteriol       Date:  1978-03       Impact factor: 3.490

10.  Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.

Authors:  K Itakura; T Hirose; R Crea; A D Riggs; H L Heyneker; F Bolivar; H W Boyer
Journal:  Science       Date:  1977-12-09       Impact factor: 47.728
View more
  20 in total

1.  Vaccination with Killed but Metabolically Active E. coli Over-expressing Hemagglutinin Elicits Neutralizing Antibodies to H1N1 Swine Origin Influenza A Virus.

Authors:  Pei-Feng Liu; Yanhan Wang; Yu-Tsueng Liu; Chun-Ming Huang
Journal:  J Nat Sci       Date:  2017-02

2.  Purification and DNA binding properties of the blaI gene product, repressor for the beta-lactamase gene, blaP, of Bacillus licheniformis.

Authors:  M J Grossman; J O Lampen
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

3.  Chimeric influenza virus hemagglutinin containing either the NH2 terminus or the COOH terminus of G protein of vesicular stomatitis virus is defective in transport to the cell surface.

Authors:  N L McQueen; D P Nayak; L V Jones; R W Compans
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

4.  Complete nucleotide sequence of the polymerase 3 gene of human influenza virus A/WSN/33.

Authors:  J S Kaptein; D P Nayak
Journal:  J Virol       Date:  1982-04       Impact factor: 5.103

5.  Characterization of a 1980-swine recombinant influenza virus possessing H1 hemagglutinin and N2 neuraminidase similar to that of the earliest Hong Kong (H3N2) virus.

Authors:  K Nerome; Y Yoshioka; S Sakamoto; H Yasuhara; A Oya
Journal:  Arch Virol       Date:  1985       Impact factor: 2.574

6.  An influenza A/H1N1/2009 hemagglutinin vaccine produced in Escherichia coli.

Authors:  José M Aguilar-Yáñez; Roberto Portillo-Lara; Gonzalo I Mendoza-Ochoa; Sergio A García-Echauri; Felipe López-Pacheco; David Bulnes-Abundis; Johari Salgado-Gallegos; Itzel M Lara-Mayorga; Yenny Webb-Vargas; Felipe O León-Angel; Ramón E Rivero-Aranda; Yuriana Oropeza-Almazán; Guillermo M Ruiz-Palacios; Manuel I Zertuche-Guerra; Rebecca M DuBois; Stephen W White; Stacey Schultz-Cherry; Charles J Russell; Mario M Alvarez
Journal:  PLoS One       Date:  2010-07-22       Impact factor: 3.240

7.  The sequence of RNA segment 1 of influenza virus A/NT/60/68 and its comparison with the corresponding segment of strains A/PR/8/34 and A/WSN/33.

Authors:  K L Jones; J A Huddleston; G G Brownlee
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

8.  Human influenza virus hemagglutinin is expressed in monkey cells using simian virus 40 vectors.

Authors:  J R Hartman; D P Nayak; G C Fareed
Journal:  Proc Natl Acad Sci U S A       Date:  1982-01       Impact factor: 11.205

9.  Hepatitis B virus polypeptide X: expression in Escherichia coli and identification of specific antibodies in sera from hepatitis B virus-infected humans.

Authors:  M L Meyers; L V Trepo; N Nath; J J Sninsky
Journal:  J Virol       Date:  1986-01       Impact factor: 5.103

10.  Solubilization and immune-detection of beta-galactosidase hybrid proteins carrying foreign antigenic determinants.

Authors:  K K Stanley
Journal:  Nucleic Acids Res       Date:  1983-06-25       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.