Literature DB >> 6292850

pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts.

U Rüther.   

Abstract

A multipurpose plasmid, pUR 250, has been constructed. It contains seven unique cloning sites (Hind III, Xba I, Sal I, Acc I, Hinc II, BamH I and EcoR I) in a small region of its lac Z gene part. Insertion of foreign DNA into the plasmid can be easily detected. Plasmid DNA isolated from a clone by a rapid method can be used to determine the DNA sequence of the insert from both ends directly without isolation of labeled fragment.

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Year:  1982        PMID： 6292850      PMCID： PMC320928          DOI： 10.1093/nar/10.19.5765

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Amino acid sequence of beta-galactosidase. XI. Peptide ordering procedures and the complete sequence.

Authors:  A V Fowler; I Zabin
Journal:  J Biol Chem       Date:  1978-08-10       Impact factor: 5.157

2.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

3.  Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product.

Authors:  N E Murray; S A Bruce; K Murray
Journal:  J Mol Biol       Date:  1979-08-15       Impact factor: 5.469

4.  pUR222, a vector for cloning and rapid chemical sequencing of DNA.

Authors:  U Rüther; M Koenen; K Otto; B Müller-Hill
Journal:  Nucleic Acids Res       Date:  1981-08-25       Impact factor: 16.971

5.  Construction and properties of a new cloning vehicle, allowing direct screening for recombinant plasmids.

Authors:  U Rüther
Journal:  Mol Gen Genet       Date:  1980

6.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  The rapid purification of T4 DNA ligase from a lambda T4 lig lysogen.

Authors:  R C Tait; R L Rodriguez; R W West
Journal:  J Biol Chem       Date:  1980-02-10       Impact factor: 5.157

8.  A system for shotgun DNA sequencing.

Authors:  J Messing; R Crea; P H Seeburg
Journal:  Nucleic Acids Res       Date:  1981-01-24       Impact factor: 16.971

9.  Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.

Authors:  F Bolivar; R L Rodriguez; M C Betlach; H W Boyer
Journal:  Gene       Date:  1977       Impact factor: 3.688

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
  10 in total
  74 in total

1.  DNA sequence of IS91 and identification of the transposase gene.

Authors:  M V Mendiola; Y Jubete; F de la Cruz
Journal:  J Bacteriol       Date:  1992-02       Impact factor: 3.490

2.  Solid phase in vitro mutagenesis using plasmid DNA template.

Authors:  T Hultman; M Murby; S Ståhl; E Hornes; M Uhlén
Journal:  Nucleic Acids Res       Date:  1990-09-11       Impact factor: 16.971

3.  Interaction between heat shock protein DnaK and recombinant staphylococcal protein A.

Authors:  H Hellebust; M Uhlén; S O Enfors
Journal:  J Bacteriol       Date:  1990-09       Impact factor: 3.490

4.  Cloning and characterization of the MboII restriction-modification system.

Authors:  H Bocklage; K Heeger; B Müller-Hill
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

5.  Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization.

Authors:  T Rygus; A Scheler; R Allmansberger; W Hillen
Journal:  Arch Microbiol       Date:  1991       Impact factor: 2.552

6.  Selection for Tn10 tet repressor binding to tet operator in Escherichia coli: isolation of temperature-sensitive mutants and combinatorial mutagenesis in the DNA binding motif.

Authors:  A Wissmann; L V Wray; U Somaggio; R Baumeister; M Geissendörfer; W Hillen
Journal:  Genetics       Date:  1991-06       Impact factor: 4.562

7.  Evaluation of staphylococcal cell surface display and flow cytometry for postselectional characterization of affinity proteins in combinatorial protein engineering applications.

Authors:  John Löfblom; Julia Sandberg; Henrik Wernérus; Stefan Ståhl
Journal:  Appl Environ Microbiol       Date:  2007-09-14       Impact factor: 4.792

8.  Site-specific integration of genes into hot spots for recombination flanking aadA in Tn21 transposons.

Authors:  E J Nücken; R B Henschke; F R Schmidt
Journal:  Mol Gen Genet       Date:  1991-09

9.  Nucleotides flanking the promoter sequence influence the transcription of the yeast mitochondrial gene coding for ATPase subunit 9.

Authors:  T K Biswas; G S Getz
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

10.  Analysis of a eukaryotic beta-galactosidase gene: the N-terminal end of the yeast Kluyveromyces lactis protein shows homology to the Escherichia coli lacZ gene product.

Authors:  K D Breunig; U Dahlems; S Das; C P Hollenberg
Journal:  Nucleic Acids Res       Date:  1984-03-12       Impact factor: 16.971
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