Literature DB >> 6322161

High-level production of biologically active human alpha 1-antitrypsin in Escherichia coli.

M Courtney, A Buchwalder, L H Tessier, M Jaye, A Benavente, A Balland, V Kohli, R Lathe, P Tolstoshev, J P Lecocq.   

Abstract

A cDNA clone containing the complete human alpha 1-antitrypsin sequence was isolated from a human liver cDNA bank by screening with a chemically synthesized oligonucleotide probe. DNA sequences encoding the alpha 1-antitrypsin mature polypeptide were inserted into an Escherichia coli expression vector that allows transcription from the efficient leftward promoter of bacteriophage lambda (PL) and initiation of translation at the lambda cII gene ribosome-binding site. This construction resulted in the induction of a 45-kilodalton protein at a level of approximately 15% of total cell protein. The polypeptide produced was recognized by antisera raised against human alpha 1-antitrypsin protein and displayed normal biological activity in an in vitro antielastase assay.

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Year:  1984        PMID： 6322161      PMCID： PMC344896          DOI： 10.1073/pnas.81.3.669

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  32 in total

1.  Characterization of alpha1-antitrypsin in the inclusion bodies from the liver in alpha 1-antitrypsin deficiency.

Authors:  J O Jeppsson; C Larsson; S Eriksson
Journal:  N Engl J Med       Date:  1975-09-18       Impact factor: 91.245

2.  Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Authors:  J Messing; B Gronenborn; B Müller-Hill; P Hans Hopschneider
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  alpha1-Antitrypsin: the presence of excess mannose in the Z variant isolated from liver.

Authors:  A Hercz; E Katona; E Cutz; J R Wilson; M Barton
Journal:  Science       Date:  1978-09-29       Impact factor: 47.728

5.  Determinant of cistron specificity in bacterial ribosomes.

Authors:  J Shine; L Dalgarno
Journal:  Nature       Date:  1975-03-06       Impact factor: 49.962

6.  Cloning and expression in Escherichia coli of full-length complementary DNA coding for human alpha 1-antitrypsin.

Authors:  A Bollen; A Herzog; A Cravador; P Hérion; P Chuchana; A Vander Straten; R Loriau; P Jacobs; A van Elsen
Journal:  DNA       Date:  1983

7.  Comparison of the chemical, physical, and survival properties of normal and Z-variant alpha-1-antitrypsins.

Authors:  R R Miller; M S Kuhlenschmidt; C J Coffee; I Kuo; R H Glew
Journal:  J Biol Chem       Date:  1976-08-10       Impact factor: 5.157

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Nucleotide sequence of cro, cII and part of the O gene in phage lambda DNA.

Authors:  E Schwarz; G Scherer; G Hobom; H Kössel
Journal:  Nature       Date:  1978-03-30       Impact factor: 49.962

10.  Cloning of several cDNA segments coding for human liver proteins.

Authors:  F Costanzo; L Castagnoli; L Dente; P Arcari; M Smith; P Costanzo; G Raugei; P Izzo; T C Pietropaolo; L Bougueleret; F Cimino; F Salvatore; R Cortese
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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  33 in total

1.  Oxidants spontaneously released by alveolar macrophages of cigarette smokers can inactivate the active site of alpha 1-antitrypsin, rendering it ineffective as an inhibitor of neutrophil elastase.

Authors:  R C Hubbard; F Ogushi; G A Fells; A M Cantin; S Jallat; M Courtney; R G Crystal
Journal:  J Clin Invest       Date:  1987-11       Impact factor: 14.808

2.  The second zinc-finger domain of poly(ADP-ribose) polymerase determines specificity for single-stranded breaks in DNA.

Authors:  G Gradwohl; J M Ménissier de Murcia; M Molinete; F Simonin; M Koken; J H Hoeijmakers; G de Murcia
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

3.  Characterization of the gene and protein of the common alpha 1-antitrypsin normal M2 allele.

Authors:  T Nukiwa; M L Brantly; F Ogushi; G A Fells; R G Crystal
Journal:  Am J Hum Genet       Date:  1988-09       Impact factor: 11.025

Review 4.  Engineering the serpin α1 -antitrypsin: A diversity of goals and techniques.

Authors:  Benjamin M Scott; William P Sheffield
Journal:  Protein Sci       Date:  2019-12-09       Impact factor: 6.725

5.  Molecular characterization of a 23-kilodalton major antigen secreted by Toxoplasma gondii.

Authors:  M F Cesbron-Delauw; B Guy; G Torpier; R J Pierce; G Lenzen; J Y Cesbron; H Charif; P Lepage; F Darcy; J P Lecocq
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

6.  Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase.

Authors:  A Giallongo; S Feo; R Moore; C M Croce; L C Showe
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

Review 7.  The purification of eukaryotic polypeptides synthesized in Escherichia coli.

Authors:  F A Marston
Journal:  Biochem J       Date:  1986-11-15       Impact factor: 3.857

8.  Molecular cloning and expression of an intracellular serpin: an elastase inhibitor from horse leucocytes.

Authors:  T Kordula; A Dubin; H Schooltink; A Koj; P C Heinrich; S Rose-John
Journal:  Biochem J       Date:  1993-07-01       Impact factor: 3.857

9.  A gene encoding arginyl-tRNA synthetase is located in the upstream region of the lysA gene in Brevibacterium lactofermentum: regulation of argS-lysA cluster expression by arginine.

Authors:  J A Oguiza; M Malumbres; G Eriani; A Pisabarro; L M Mateos; F Martin; J F Martín
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490

10.  High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts.

Authors:  M Nadai; J Bally; M Vitel; C Job; G Tissot; J Botterman; M Dubald
Journal:  Transgenic Res       Date:  2008-08-07       Impact factor: 2.788
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