Literature DB >> 6282812

Bacillus subtilis DNA gyrase: purification of subunits and reconstitution of supercoiling activity.

E Orr, W L Staudenbauer.   

Abstract

DNA gyrase from Bacillus subtilis 168 was purified by affinity chromatography on novobiocin-Sepharose and shown to consist of two subunits, A and B, with molecular weights of 100,000 and 85,000, respectively. The B subunits, which contains novobiocin-sensitive. ATPase activity, could complement the gyrA protein of Escherichia coli. No complementation was detected between the A subunit and the E. coli gyrB protein.

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Year:  1982        PMID： 6282812      PMCID： PMC220277          DOI： 10.1128/jb.151.1.524-527.1982

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  12 in total

1.  DNA gyrase: an enzyme that introduces superhelical turns into DNA.

Authors:  M Gellert; K Mizuuchi; M H O'Dea; H A Nash
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

2.  Isolation and characterisation of a strain carrying a conditional lethal mutation in the cou gene of Escherichia coli K12.

Authors:  E Orr; N F Fairweather; I B Holland; R H Pritchard
Journal:  Mol Gen Genet       Date:  1979

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Deoxyribonucleic acid gyrase-deoxyribonucleic acid complex containing 140 base pairs of deoxyribonucleic acid and an alpha 2 beta 2 protein core.

Authors:  L Klevan; J C Wang
Journal:  Biochemistry       Date:  1980-11-11       Impact factor: 3.162

5.  Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.

Authors:  T Spector
Journal:  Anal Biochem       Date:  1978-05       Impact factor: 3.365

Review 6.  DNA gyrase and the supercoiling of DNA.

Authors:  N R Cozzarelli
Journal:  Science       Date:  1980-02-29       Impact factor: 47.728

7.  Bacillus subtilis deoxyribonucleic acid gyrase.

Authors:  A Sugino; K F Bott
Journal:  J Bacteriol       Date:  1980-03       Impact factor: 3.490

8.  Micrococcus luteus DNA gyrase: active components and a model for its supercoiling of DNA.

Authors:  L F Liu; J C Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205

9.  Replication of phi X174 dna with purified enzymes. I. Conversion of viral DNA to a supercoiled, biologically active duplex.

Authors:  J Shlomai; L Polder; K Arai; A Kornberg
Journal:  J Biol Chem       Date:  1981-05-25       Impact factor: 5.157

10.  An Escherichia coli mutant thermosensitive in the B subunit of DNA gyrase: effect on the structure and replication of the colicin E1 plasmid in vitro.

Authors:  E Orr; W L Staudenbauer
Journal:  Mol Gen Genet       Date:  1981
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  22 in total

1.  Drug-induced relaxation of supercoiled plasmid DNA in Bacillus subtilis and induction of the SOS response.

Authors:  M S Osburne; S M Zavodny; G A Peterson
Journal:  J Bacteriol       Date:  1988-01       Impact factor: 3.490

2.  RemA (YlzA) and RemB (YaaB) regulate extracellular matrix operon expression and biofilm formation in Bacillus subtilis.

Authors:  Jared T Winkelman; Kris M Blair; Daniel B Kearns
Journal:  J Bacteriol       Date:  2009-04-10       Impact factor: 3.490

3.  The effect of novobiocin on yeast topoisomerase type II.

Authors:  M J Pocklington; J R Jenkins; E Orr
Journal:  Mol Gen Genet       Date:  1990-01

Review 4.  Biochemical characteristics and physiological significance of major DNA topoisomerases.

Authors:  J A Sutcliffe; T D Gootz; J F Barrett
Journal:  Antimicrob Agents Chemother       Date:  1989-12       Impact factor: 5.191

5.  Some properties of subunits of DNA gyrase from Pseudomonas aeruginosa PAO1 and its nalidixic acid-resistant mutant.

Authors:  Y Inoue; K Sato; T Fujii; K Hirai; M Inoue; S Iyobe; S Mitsuhashi
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490

6.  Purification and properties of DNA gyrase from Vibrio cholerae.

Authors:  G Mukhopadhyay; A Ghosh
Journal:  Biochem J       Date:  1991-12-15       Impact factor: 3.857

7.  Mechanisms of clinical resistance to fluoroquinolones in Staphylococcus aureus.

Authors:  N Nakanishi; S Yoshida; H Wakebe; M Inoue; T Yamaguchi; S Mitsuhashi
Journal:  Antimicrob Agents Chemother       Date:  1991-12       Impact factor: 5.191

8.  Sequence analysis, purification, and study of inhibition by 4-quinolones of the DNA gyrase from Mycobacterium smegmatis.

Authors:  V Revel-Viravau; Q C Truong; N Moreau; V Jarlier; W Sougakoff
Journal:  Antimicrob Agents Chemother       Date:  1996-09       Impact factor: 5.191

9.  DNA gyrase activity regulates DnaA-dependent replication initiation in Bacillus subtilis.

Authors:  A N Samadpour; H Merrikh
Journal:  Mol Microbiol       Date:  2018-03-06       Impact factor: 3.501

10.  Mechanisms of clinical resistance to fluoroquinolones in Enterococcus faecalis.

Authors:  N Nakanishi; S Yoshida; H Wakebe; M Inoue; S Mitsuhashi
Journal:  Antimicrob Agents Chemother       Date:  1991-06       Impact factor: 5.191
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